In:
FEBS Letters, Wiley, Vol. 148, No. 2 ( 1982-11-08), p. 302-306
Abstract:
Phenobarbital‐inducible cytochrome P450 variants have a higher affinity (app. K s = 0.6 μM) for metyrapone than do non‐phenobarbital‐inducible variants (app. K s = 80 μM). The difference in affinity has enabled us to quantitate, by metyrapone‐induced difference spectral analysis, phenobarbital‐inducible cytochromes P450 in liver microsomal membranes. The result obtained by this simple, rapid technique compare well with those found by more sophisticated techniques such as radioimmunoassay and immunoprecipitation.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(82)80829-6
Language:
English
Publisher:
Wiley
Publication Date:
1982
detail.hit.zdb_id:
1460391-3
SSG:
12
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