In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 69, No. 4 ( 2019-04-01), p. 1016-1023
Abstract:
Cells of bacterial strains 4 G-K06 T and 4MSK11 T , isolated from soil samples collected from monsoon evergreen broad-leaved forest of the Dinghushan Mountain (112° 31′ E 23° 10′ N), Guangdong Province, PR China, were Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped. Strain 4 G-K06 T grew at 10–37 °C, pH 3.5–7.5 and 0–3.5 % (w/v) NaCl; while 4MSK11 T grew at 4–42 °C, pH 3.5–7.5 and 0–2.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 4 G-K06 T formed a clade with Dyella flagellata 4 M-K16 T , Dyella acidisoli 4M-Z03 T , Dyella humi DHG40 T and Dyella nitratireducens DHG59 T , while strain 4MSK11 T formed a clade with Dyella caseinilytica DHOB09 T and Dyella mobilis DHON07 T , both within the genus Dyella . The result of the partial atpD , gyrB and lepA gene sequence analysis supported the conclusion based on 16S rRNA gene sequence analysis, which showed that these two strains represent two novel species of Dyella . The average nucleotide identity and digital DNA–DNA hybridization value for the whole genomes were 75.0–79.0 and 20.3–22.6 % between strains 4 G-K06 T , 4MSK11 T and those described Dyella species with genome sequences; while the DNA–DNA hybridization rates between strains 4 G-K06 T , 4MSK11 T and closely related Dyella species (without genome sequence) were 29.5–41.8 %. The major cellular fatty acids of these two strains were iso-C 15 : 0 , iso-C 16 : 0 and iso-C 17 : 1 ω 9 c , while the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids and aminophospholipids. The only ubiquinone of these two strains was ubiquinone-8. The DNA G+C contents of 4 G-K06 T and 4MSK11 T were 60.4 and 61.3 mol%, respectively. On the basis of the evidence presented here, strains 4 G-K06 T and 4MSK11 T represent two novel species of the genus Dyella , for which the names Dyella monticola sp. nov. (type strain 4 G-K06 T =LMG 30268 T =GDMCC 1.1188 T ) and Dyella psychrodurans sp. nov. (type strain 4MSK11 T =KCTC 62280 T =GDMCC 1.1185 T ) are proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.003259
Language:
English
Publisher:
Microbiology Society
Publication Date:
2019
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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