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  • 1
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    Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy
    Springer Science and Business Media LLC ; 2020
    In:  BMC Medical Genomics Vol. 13, No. 1 ( 2020-12)
    Details
    In: BMC Medical Genomics, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2020-12)
    Abstract: Angiogenesis is an important parameter in the development of diabetic retinopathy (DR), and it is indicative of an early stage evolving into a late phase. Therefore, examining the role of angiogenic factors in early DR is crucial to understanding the mechanism of neovascularization. Methods The present study identified hub genes and pathways associated with angiogenesis in early DR using bioinformatics analysis. Genes from published literature and Gene Expression Omnibus (GEO) were collected and analysed. Results We collected 73 genes from 70 published studies in PubMed, which were referred to as DR-related gene set 1 (DRgset1). The gene expression profile of GSE12610 was downloaded, and 578 differentially expressed genes (DEGs) between diabetic and normal samples were identified. DEGs and DRgset1 were further combined to create DR-related gene set 2 (DRgset2). After an enrichment analysis, we identified 12 GO terms and 2 pathways associated with neovascularization in DRgset1, and 8 GO terms and 2 pathways in DRgset2. We found 39 new genes associated with angiogenesis and verified 8 candidate angiogenesis-related genes in DR cells using real-time PCR: PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13 , and AIF1 . We identified 10 hub genes associated with neovascularization by constructing a protein-protein interaction (PPI) network: TNF, VEGFA, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1. Conclusions The present study analysed angiogenesis-related genes and pathways in early DR in a comprehensive and systematic manner. PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13 , and AIF1 may be the candidate genes to further explore the mechanisms of angiogenesis in early DR. TNF, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1 may be new targets for early neovascularization therapy in the future.
    Type of Medium: Online Resource
    ISSN: 1755-8794
    URL: Article
    DOI: 10.1186/s12920-020-00799-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2411865-5
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  • 2
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    Enhanced X-ray diffraction of in vivo -grown μNS crystals by viscous jets at XFELs
    International Union of Crystallography (IUCr) ; 2020
    In:  Acta Crystallographica Section F Structural Biology Communications Vol. 76, No. 6 ( 2020-06-01), p. 278-289
    Details
    In: Acta Crystallographica Section F Structural Biology Communications, International Union of Crystallography (IUCr), Vol. 76, No. 6 ( 2020-06-01), p. 278-289
    Abstract: μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories in host cells, and the μNS protein has been suggested to be the minimal viral factor required for factory formation. Thus, determining the structure of μNS is of great importance for understanding its role in viral infection. In the study presented here, a fragment consisting of residues 448–605 of μNS was expressed as an EGFP fusion protein in Sf9 insect cells. EGFP-μNS (448–605) crystallization in Sf9 cells was monitored and verified by several imaging techniques. Cells infected with the EGFP-μNS (448–605) baculovirus formed rod-shaped microcrystals (5–15 µm in length) which were reconstituted in high-viscosity media (LCP and agarose) and investigated by serial femtosecond X-ray diffraction using viscous jets at an X-ray free-electron laser (XFEL). The crystals diffracted to 4.5 Å resolution. A total of 4227 diffraction snapshots were successfully indexed into a hexagonal lattice with unit-cell parameters a  = 109.29, b = 110.29, c = 324.97 Å. The final data set was merged and refined to 7.0 Å resolution. Preliminary electron-density maps were obtained. While more diffraction data are required to solve the structure of μNS (448–605) , the current experimental strategy, which couples high-viscosity crystal delivery at an XFEL with in cellulo crystallization, paves the way towards structure determination of the μNS protein.
    Type of Medium: Online Resource
    ISSN: 2053-230X
    URL: Article
    URL: Article
    DOI: 10.1107/S2053230X20006172
    DOI: 10.1107/S2053230X20006172/uf5001sup1.pdf
    Language: Unknown
    Publisher: International Union of Crystallography (IUCr)
    Publication Date: 2020
    detail.hit.zdb_id: 2175956-X
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  • 3
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    An improved two-steps method for secondary in-the-bag intraocular lens implantation in aphakia eye
    SAGE Publications ; 2023
    In:  European Journal of Ophthalmology Vol. 33, No. 2 ( 2023-03), p. 1239-1243
    Details
    In: European Journal of Ophthalmology, SAGE Publications, Vol. 33, No. 2 ( 2023-03), p. 1239-1243
    Abstract: To describe an improved technique for secondary IOL implantation in constricted capsular bag in aphakic eyes. Setting This study was designed and carried out in the department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital). Design This is a retrospective study which evaluate the post-operative effect of the two-steps secondary IOL implantation. Method 21 eyes of 21 patients who underwent primary cataract surgery from June 2020 to September 2020 were enrolled. Two-steps IOL implantation was performed. Patients were followed up with ophthalmic examinations. Results Of all 21 eyes, the capsular bags were reopened and the IOL were implanted into the bag. During follow-up, the mean age was 62.3 ± 7.3 (range, 44–81) years and mean interval between the primary surgery and secondary IOL implantation was 23.5 ± 9.6 (range, 4.1–78.3) weeks. A marked improvement of postoperative mean BCVA was noticed (pre 20/84 Snellen, 0.62 ± 0.26 logMAR equivalent, and post 20/44 Snellen, 0.34 ± 0.33 logMAR equivalent; p = 0.001). The mean SE also improved from + 10.32 ± 5.05D at baseline to −2.68 ± 1.36D at the last follow-up time ( p  〈  0.001). The mean IOP showed no significant difference (pre 17.62 ± 4.66 mmHg, post16.84 ± 4.73 mmHg; p = 0.6860) and the corneal endothelial cell density decreased about 11.04 ± 10.84% (from 2483 ± 218.43 cells/mm 2 to 2190 ± 361.36 cells/mm 2 p = 0.0029) Conclusion The two-steps capsular bag reopening method can be achieved in majority of cases with stable IOL position and better visual outcome.
    Type of Medium: Online Resource
    ISSN: 1120-6721 , 1724-6016
    URL: Article
    DOI: 10.1177/11206721221143607
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 1475018-1
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  • 4
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    Highly efficient and stable ZnO-based perovskite solar cells enabled by a self-assembled monolayer as the interface linker
    Royal Society of Chemistry (RSC) ; 2022
    In:  Chemical Communications Vol. 58, No. 66 ( 2022), p. 9266-9269
    Details
    In: Chemical Communications, Royal Society of Chemistry (RSC), Vol. 58, No. 66 ( 2022), p. 9266-9269
    Abstract: 2-TA and 3-TA were introduced for the first time on the surface of ZnO, and used as SAMs for interfacial modification. A highest PCE of 20.6% was achieved for 2-TA PSCs with improved energy alignment and perovskite film quality, and reduced defect density. The modified ZnO exhibited better thermostability of the perovskite and resultant device stability.
    Type of Medium: Online Resource
    ISSN: 1359-7345 , 1364-548X
    URL: Article
    DOI: 10.1039/D2CC03890K
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2022
    detail.hit.zdb_id: 1472881-3
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  • 5
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    Inverted internal limiting membrane flap technique for large macular holes: a systematic review and single-arm meta-analysis
    Springer Science and Business Media LLC ; 2018
    In:  Graefe's Archive for Clinical and Experimental Ophthalmology Vol. 256, No. 6 ( 2018-6), p. 1041-1049
    Details
    In: Graefe's Archive for Clinical and Experimental Ophthalmology, Springer Science and Business Media LLC, Vol. 256, No. 6 ( 2018-6), p. 1041-1049
    Type of Medium: Online Resource
    ISSN: 0721-832X , 1435-702X
    URL: Article
    DOI: 10.1007/s00417-018-3956-2
    RVK:
    XA 10000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 1459159-5
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  • 6
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    Dihydroartemisinin ameliorates retinal vascular dysfunction in diabetes mellitus via the FASN/Kmal-mTOR/SREBP1 feedback loop
    Elsevier BV ; 2021
    In:  Pharmacological Research Vol. 174 ( 2021-12), p. 105871-
    Details
    In: Pharmacological Research, Elsevier BV, Vol. 174 ( 2021-12), p. 105871-
    Type of Medium: Online Resource
    ISSN: 1043-6618
    URL: Article
    DOI: 10.1016/j.phrs.2021.105871
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 1471456-5
    SSG: 15,3
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  • 7
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    High Conductivity, Semiconducting, and Metallic PEDOT:PSS Electrode for All-Plastic Solar Cells
    MDPI AG ; 2023
    In:  Molecules Vol. 28, No. 6 ( 2023-03-21), p. 2836-
    Details
    In: Molecules, MDPI AG, Vol. 28, No. 6 ( 2023-03-21), p. 2836-
    Abstract: Plastic electrodes are desirable for the rapid development of flexible organic electronics. In this article, a plastic electrode has been prepared by employing traditional conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and plastic substrate polyethersulfone (PES). The completed electrode (Denote as HC-PEDOT:PSS) treated by 80% concentrated sulfuric acid (H2SO4) possesses a high electrical conductivity of over 2673 S/cm and a high transmittance of over 90% at 550 nm. The high conductivity is attributed to the regular arrangement of PEDOT molecules, which has been proved by the X-ray diffraction characterization. Temperature-dependent conductivity measurement reveals that the HC-PEDOT:PSS possesses both semiconducting and metallic properties. The binding force and effects between the PEDOT and PEI are investigated in detail. All plastic solar cells with a classical device structure of PES/HC-PEDOT:PSS/PEI/P3HT:ICBA/EG-PEDOT:PSS show a PCE of 4.05%. The ITO-free device with a structure of Glass/HC-PEDOT:PSS/Al4083/PM6:Y6/PDINO/Ag delivers an open-circuit voltage (VOC) of 0.81 V, short-circuit current (JSC ) of 23.5 mA/cm2, fill factor (FF) of 0.67 and a moderate power conversion efficiency (PCE) of 12.8%. The above results demonstrate the HC-PEDOT:PSS electrode is a promising candidate for all-plastic solar cells and ITO-free organic solar cells.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules28062836
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2008644-1
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  • 8
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    The Role of the AIMP1 Pathway in Diabetic Retinopathy: AIMP1-Targeted Intervention Study in Diabetic Retinopathy
    S. Karger AG ; 2020
    In:  Ophthalmic Research Vol. 63, No. 2 ( 2020), p. 122-132
    Details
    In: Ophthalmic Research, S. Karger AG, Vol. 63, No. 2 ( 2020), p. 122-132
    Abstract: 〈 b 〉 〈 i 〉 Introduction: 〈 /i 〉 〈 /b 〉 We characterized the role of aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1) in retinal inflammation and apoptosis regulation, both in vivo and in vitro. In addition, we used clinical specimens to show the relationship between AIMP1 and the development of diabetic retinopathy (DR). 〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 To elucidate the role of AIMP1 in DR. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 A diabetic AIMP1-specific knockout (KO) C57 mouse model was used. Human retinal microvascular endothelial cells (HRMECs) were incubated with normal glucose, high glucose (HG), and HG + AIMP1-small interfering RNA (siRNA). The expression of AIMP1 and relative inflammatory and apoptotic cytokines in diabetic mice retina and HRMECs were measured using Western blotting and polymerase chain reaction. The apoptosis of HRMECs was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The levels of AIMP1 in the vitreous humor and serum were determined using ELISA. Possible correlations between the intravitreal level of AIMP1 and blood glucose, glycosylated hemoglobin HbA1c, intravitreal levels of IL-1β, and caspase-3 were determined. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The expression of inflammatory and apoptotic proteins was inhibited in the AIMP1 KO mice and HRMECs incubated with AIMP1-siRNA. The apoptosis of HRMECs was decreased in the AIMP1-siRNA group. The intravitreal level of AIMP1 in DR patients was significantly higher than that in nondiabetic patients ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.01). There was a positive correlation between intravitreal AIMP1 and HbA1c and intravitreal IL-1β and caspase-3 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05). 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 HG induced increased expression of AIMP1 in HRMECs and retinas from diabetic C57 mice, thereby increasing the expression of inflammatory and apoptotic cytokines, which promoted DR progression. A decrease in AIMP1 expression prevented the development of DR by inhibiting the activation of inflammatory and apoptotic signaling. Therefore, AIMP1 is an effective interfering target for the prevention and treatment of DR.
    Type of Medium: Online Resource
    ISSN: 0030-3747 , 1423-0259
    URL: Article
    DOI: 10.1159/000503637
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2020
    detail.hit.zdb_id: 1483177-6
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  • 9
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    Research Progress of circRNAs in Inflammatory Mechanisms of Diabetic Retinopathy: An Emerging Star with Potential Therapeutic Targets
    Informa UK Limited ; 2022
    In:  Current Eye Research Vol. 47, No. 2 ( 2022-02-01), p. 165-178
    Details
    In: Current Eye Research, Informa UK Limited, Vol. 47, No. 2 ( 2022-02-01), p. 165-178
    Type of Medium: Online Resource
    ISSN: 0271-3683 , 1460-2202
    URL: Article
    DOI: 10.1080/02713683.2021.1995002
    RVK:
    XA 10000
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2022
    detail.hit.zdb_id: 1483048-6
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  • 10
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    The cause of redetachment after vitrectomy with air tamponade for a cohort of 1715 patients with retinal detachment: an analysis of retinal breaks reopening
    Springer Science and Business Media LLC ; 2023
    In:  Eye and Vision Vol. 10, No. 1 ( 2023-02-03)
    Details
    In: Eye and Vision, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2023-02-03)
    Abstract: To investigate the prevalence and predictors of retinal breaks reopening after vitrectomy with air tamponade in rhegmatogenous retinal detachment (RRD). Methods A retrospective cohort study was conducted in Shanghai General Hospital. Chart review was performed among 1715 patients with primary RRD who received pars plana vitrectomy (PPV) with air tamponade as initial management. Patients were followed up for recurrence. The clinical features of the eyes with retinal breaks reopening were recorded. Logistic regression was constructed to investigate the predictors for breaks reopening. Results A total of 137 (7.99%) patients had recurrent retinal detachment after PPV with air tamponade. The causes of surgery failure included new or missed retinal breaks (48.9%), reopening of original tears (43.8%) and proliferative vitreoretinopathy (7.3%). The median time to recurrence for the patients with breaks reopening was 18.0 days. Multivariate logistic regression indicated that the presence of retinal break(s) ≥ 1.5 disc diameters (DD) (odds ratio [OR]: 2.68, 95% confidence interval [CI] : 11.04–6.92, P  = 0.041), and shorter period for restricted activities (OR: 0.94, 95% CI: 0.89–0.99, P  = 0.020) were the independent predictors for breaks reopening. Conclusions Breaks reopening is an important cause for retinal redetachment after PPV with air tamponade in primary RRD. The first 2–4 weeks after surgery is the “risk period” for breaks reopening. Special attention should be paid for patients with retinal break(s) ≥ 1.5 DD. A prolonged period for restricted activities is recommended.
    Type of Medium: Online Resource
    ISSN: 2326-0254
    URL: Article
    DOI: 10.1186/s40662-022-00325-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2806610-8
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