In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 9 ( 2022-9-16), p. e3001777-
Abstract:
Wound healing in the skin is a complex physiological process that is a product of a cell state transition from homeostasis to repair. Mechanical cues are increasingly being recognized as important regulators of cellular reprogramming, but the mechanism by which it is translated to changes in gene expression and ultimately cellular behavior remains largely a mystery. To probe the molecular underpinnings of this phenomenon further, we used the down-regulation of caspase-8 as a biomarker of a cell entering the wound healing program. We found that the wound-induced release of tension within the epidermis leads to the alteration of gene expression via the nuclear translocation of the DNA methyltransferase 3A (DNMT3a). This enzyme then methylates promoters of genes that are known to be down-regulated in response to wound stimuli as well as potentially novel players in the repair program. Overall, these findings illuminate the convergence of mechanical and epigenetic signaling modules that are important regulators of the transcriptome landscape required to initiate the tissue repair process in the differentiated layers of the epidermis.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001777
DOI:
10.1371/journal.pbio.3001777.g001
DOI:
10.1371/journal.pbio.3001777.g002
DOI:
10.1371/journal.pbio.3001777.g003
DOI:
10.1371/journal.pbio.3001777.g004
DOI:
10.1371/journal.pbio.3001777.t001
DOI:
10.1371/journal.pbio.3001777.s001
DOI:
10.1371/journal.pbio.3001777.s002
DOI:
10.1371/journal.pbio.3001777.s003
DOI:
10.1371/journal.pbio.3001777.s004
DOI:
10.1371/journal.pbio.3001777.s005
DOI:
10.1371/journal.pbio.3001777.s006
DOI:
10.1371/journal.pbio.3001777.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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