In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2021-7-29), p. e1009797-
Abstract:
Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii . Each of these organisms express two PanK homologues (PanK1 and PanK2). We demonstrate that Pf PanK1 and Pf PanK2 associate, forming a single, functional PanK complex that includes the multi-functional protein, Pf 14-3-3I. Similarly, we demonstrate that Tg PanK1 and Tg PanK2 form a single complex that possesses PanK activity. Both Tg PanK1 and Tg PanK2 are essential for T . gondii proliferation, specifically due to their PanK activity. Our study constitutes the first examples of heteromeric PanK complexes in nature and provides an explanation for the presence of multiple PanKs within certain organisms.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009797
DOI:
10.1371/journal.ppat.1009797.g001
DOI:
10.1371/journal.ppat.1009797.g002
DOI:
10.1371/journal.ppat.1009797.g003
DOI:
10.1371/journal.ppat.1009797.g004
DOI:
10.1371/journal.ppat.1009797.g005
DOI:
10.1371/journal.ppat.1009797.s001
DOI:
10.1371/journal.ppat.1009797.s002
DOI:
10.1371/journal.ppat.1009797.s003
DOI:
10.1371/journal.ppat.1009797.s004
DOI:
10.1371/journal.ppat.1009797.s005
DOI:
10.1371/journal.ppat.1009797.s006
DOI:
10.1371/journal.ppat.1009797.s007
DOI:
10.1371/journal.ppat.1009797.s008
DOI:
10.1371/journal.ppat.1009797.s009
DOI:
10.1371/journal.ppat.1009797.s010
DOI:
10.1371/journal.ppat.1009797.s011
DOI:
10.1371/journal.ppat.1009797.s012
DOI:
10.1371/journal.ppat.1009797.s013
DOI:
10.1371/journal.ppat.1009797.s014
DOI:
10.1371/journal.ppat.1009797.s015
DOI:
10.1371/journal.ppat.1009797.s016
DOI:
10.1371/journal.ppat.1009797.s017
DOI:
10.1371/journal.ppat.1009797.s018
DOI:
10.1371/journal.ppat.1009797.r001
DOI:
10.1371/journal.ppat.1009797.r002
DOI:
10.1371/journal.ppat.1009797.r003
DOI:
10.1371/journal.ppat.1009797.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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