In:
Journal of Cellular and Molecular Medicine, Wiley, Vol. 19, No. 8 ( 2015-08), p. 1783-1794
Abstract:
Telocytes ( TC s) are interstitial cells that are present in numerous organs, including the heart interstitial space and cardiac stem cell niche. TCs are completely different from fibroblasts. TC s release extracellular vesicles that may interact with cardiac stem cells ( CSC s) via paracrine effects. Data on the secretory profile of TC s and the bidirectional shuttle vesicular signalling mechanism between TC s and CSC s are scarce. We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate. Therefore, we studied the protein secretory profile using supernatants from mouse cultured cardiac TC s. We also performed a comparative secretome analysis using supernatants from rat cultured cardiac TC s, a pure CSC line and TC s‐ CSC s in co‐culture using ( i ) high‐sensitivity on‐chip electrophoresis, ( ii ) surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry and ( iii ) multiplex analysis by Luminex‐ xMAP . We identified several highly expressed molecules in the mouse cardiac TC secretory profile: interleukin ( IL )‐6, VEGF , macrophage inflammatory protein 1α ( MIP ‐1α), MIP ‐2 and MCP ‐1, which are also present in the proteome of rat cardiac TC s. In addition, rat cardiac TC s secrete a slightly greater number of cytokines, IL ‐2, IL ‐10, IL ‐13 and some chemokines like, GRO ‐ KC . We found that VEGF , IL ‐6 and some chemokines (all stimulated by IL ‐6 signalling) are secreted by cardiac TC s and overexpressed in co‐cultures with CSC s. The expression levels of MIP ‐2 and MIP ‐1α increased twofold and fourfold, respectively, when TC s were co‐cultured with CSC s, while the expression of IL ‐2 did not significantly differ between TC s and CSC s in mono culture and significantly decreased (twofold) in the co‐culture system. These data suggest that the TC secretome plays a modulatory role in stem cell proliferation and differentiation.
Type of Medium:
Online Resource
ISSN:
1582-1838
,
1582-4934
DOI:
10.1111/jcmm.2015.19.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2076114-4
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