In:
Development, The Company of Biologists, Vol. 111, No. 3 ( 1991-03-01), p. 647-655
Abstract:
Experiments were done to test the hypothesis that the avian gut is colonized by cells derived from both vagal and sacral regions of the neural crest. A fluorescent dye, dil (l,l-dioctadecyl-3,3,3′,3′-tetramethylmdocarbocya-nine perchlorate), and a replication-deficient retrovirus (LZ10; Galileo et al. 1990) were employed as tracers. Since LZ10 was constructed with lacZ of E. coli as a reporter gene, infected cells were identified by demonstrating β-galactosidase immunoreactivity. Dil and LZ10 were injected between the neural tube and surface ectoderm (before the migration of crest cells away from the injection sites) at vagal, truncal (dil only), or sacral axial levels. The bowel was examined 4 days later in order to allow crest-derived cells sufficient time to migrate to the gut. Following injections of either tracer into the vagal crest, labelled cells were found in the gizzard and duodenum.’When dil or LZ10 was injected into the sacral crest, labelled cells were seen in the postumbilical bowel and ganglion of Remak. In the hindgut, marked cells were concentrated in the mesenchyme, just internal to the serosa, and were never observed rostral to the umbilicus. No fluorescent cells were ever found in the bowel following truncal injections of dil, although such cells were observed in sympathetic ganglia. Labelled cells were always found in dorsal root ganglia, no matter which tracer or level of the crest was injected. In embryos injected with LZ10, infected cells in the gut and dorsal root ganglia displayed a neural crest marker (NC-1 immunoreactivity). These observations confirm that the gut is colonized by cells from the sacral as well as the vagal region of the neural crest and that the émigrés from the sacral crest are confined to the post-umbilical bowel.
Type of Medium:
Online Resource
ISSN:
0950-1991
,
1477-9129
DOI:
10.1242/dev.111.3.647
Language:
English
Publisher:
The Company of Biologists
Publication Date:
1991
detail.hit.zdb_id:
2007916-3
SSG:
12
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