In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2022-8-23), p. e0269823-
Abstract:
COVID-19 pandemic has accelerated the development of vaccines against its etiologic agent, SARS-CoV-2. However, the emergence of new variants of the virus lead to the generation of new alternatives to improve the current sub-unit vaccines in development. In the present report, the immunogenicity of the Spike RBD of SARS-CoV-2 formulated with an oil-in-water emulsion and a water-in-oil emulsion with squalene was evaluated in mice and hamsters. The RBD protein was expressed in insect cells and purified by chromatography until 〉 95% purity. The protein was shown to have the appropriate folding as determined by ELISA and flow cytometry binding assays to its receptor, as well as by its detection by hamster immune anti-S1 sera under non-reducing conditions. In immunization assays, although the cellular immune response elicited by both adjuvants were similar, the formulation based in water-in-oil emulsion and squalene generated an earlier humoral response as determined by ELISA. Similarly, this formulation was able to stimulate neutralizing antibodies in hamsters. The vaccine candidate was shown to be safe, as demonstrated by the histopathological analysis in lungs, liver and kidney. These results have shown the potential of this formulation vaccine to be evaluated in a challenge against SARS-CoV-2 and determine its ability to confer protection.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0269823
DOI:
10.1371/journal.pone.0269823.g001
DOI:
10.1371/journal.pone.0269823.g002
DOI:
10.1371/journal.pone.0269823.g003
DOI:
10.1371/journal.pone.0269823.g004
DOI:
10.1371/journal.pone.0269823.g005
DOI:
10.1371/journal.pone.0269823.g006
DOI:
10.1371/journal.pone.0269823.g007
DOI:
10.1371/journal.pone.0269823.g008
DOI:
10.1371/journal.pone.0269823.s001
DOI:
10.1371/journal.pone.0269823.s002
DOI:
10.1371/journal.pone.0269823.s003
DOI:
10.1371/journal.pone.0269823.s004
DOI:
10.1371/journal.pone.0269823.s005
DOI:
10.1371/journal.pone.0269823.s006
DOI:
10.1371/journal.pone.0269823.s007
DOI:
10.1371/journal.pone.0269823.s008
DOI:
10.1371/journal.pone.0269823.r001
DOI:
10.1371/journal.pone.0269823.r002
DOI:
10.1371/journal.pone.0269823.r003
DOI:
10.1371/journal.pone.0269823.r004
DOI:
10.1371/journal.pone.0269823.r005
DOI:
10.1371/journal.pone.0269823.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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