In:
BJU International, Wiley, Vol. 112, No. 5 ( 2013-09), p. 686-696
Kurzfassung:
To identify a phenotype that could be informative and prognostic in patients with renal cell carcinoma ( RCC ) peripheral blood was evaluated for TH 1, TH 2, regulatory T cells ( T regs), natural killer ( NK ) and NKT cells and for cytokines/chemokines. Patients and Methods Peripheral blood from 77 patients with RCC and 40 healthy controls was evaluated by flow cytometry using monoclonal antibodies against CD 4, CD 25, F ox P 3, CD45RA , CD45RO , CD152 , CD184 , CD279 , CD3 , CD16 , CD56 , CD161 , CD158a , CD4 , CD26 , CD30 , CD183 and CD184 . A concomitant evaluation of 38 molecules was conducted in patients’ serum using a multiplex biometric ELISA ‐based immunoassay. Results The number of NK cells CD3 − / CD16 + , CD3 − / CD16 + / CD161 + ( NK ) and CD3 − / CD16 + / CD161 + / CD158a + ( NK ‐ Kir 2+) was greater in the patients with RCC ( P 〈 0.05); and the number of Treg cells CD4 + / CD25 high + / FOXP3 + and the subset CD4 + / CD25 high + / FOXP3 + / CD45RA + (naïve) and CD45R0 + (memory) cells, were greater in the patients with RCC ( P 〈 0.001). An increase in the following was observed in the serum of patients with RCC compared with healthy controls: interleukin ( IL )‐4, IL ‐6, IL ‐8, IL ‐10, G ‐ CSF , CXCL10 , CXCL11 , hepatocyte growth factor ( HGF ) and vascular endothelial growth factor ( VEGF ). According to Ingenuity Pathway Analysis ( IPA ), CXCL10 , IL ‐6, IL ‐8, epidermal growth factor ( EGF ), HGF and VEGF were associated with a network that controls cellular movement, tissue development and cellular growth. Kaplan–Meier analysis for disease‐free survival showed that high numbers of CD4 + / CD25 high + / FOXP3 + / CD45RA + (Treg naïve) and low numbers of CD3 − / CD16 + / CD161 + / CD158a + ( NK ‐ Kir +) cells predict short disease‐free survival in patients with RCC . Conclusion Concomitant evaluation of T reg ( CD4 + / CD25 high + / FOXP3 + and CD4 + / CD25 high + / FOXP3 + / CD45RA + ) and of six soluble factors ( IL ‐6, IL ‐8 , VEGF , CXCL10 , CXCL11 , EGF , HGF ) might be a surrogate marker of host immunity in patients with RCC .
Materialart:
Online-Ressource
ISSN:
1464-4096
,
1464-410X
DOI:
10.1111/bju.2013.112.issue-5
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2013
ZDB Id:
2019983-1
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