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  • 1
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 191, No. 9 ( 2009-05), p. 2953-2963
    Abstract: The repressor CodY is reported to inhibit metabolic genes mainly involved in nitrogen metabolism. We analyzed codY mutants from three unrelated Staphylococcus aureus strains (Newman, UAMS-1, and RN1HG). The mutants grew more slowly than their parent strains in a chemically defined medium. However, only codY mutants were able to grow in medium lacking threonine. An excess of isoleucine resulted in growth inhibition in the wild type but not in the codY mutants, indicating that isoleucine plays a role in CodY-dependent repression. Prototypic CodY-repressed genes including the virulence regulator agr are repressed after up-shift with isoleucine. The CodY-dependent repression of agr is consistent with the concomitant influence of CodY on typical agr -regulated genes such as cap , spa , fnbA , and coa . However, some of these virulence genes (e.g., cap , fnbA , and spa ) were also regulated by CodY in an agr- negative background. Microarray analysis revealed that the large majority of CodY-repressed genes were involved in amino acid metabolism; CodY-activated genes were mainly involved in nucleotide metabolism or virulence. In summary, CodY in S. aureus not only acts as a repressor for genes involved in nitrogen metabolism but also contributes to virulence gene regulation by supporting as well as substituting for agr function.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2022
    In:  The European Physical Journal A Vol. 58, No. 12 ( 2022-12-16)
    In: The European Physical Journal A, Springer Science and Business Media LLC, Vol. 58, No. 12 ( 2022-12-16)
    Abstract: We present the design, prototype developments and test results of the new time-of-flight detector (ToFD) which is part of the R $$^3$$ 3 B experimental setup at GSI and FAIR, Darmstadt, Germany. The ToFD detector is able to detect heavy-ion residues of all charges at relativistic energies with a relative energy precision $$\sigma _{\varDelta E}/{\varDelta E}$$ σ Δ E / Δ E of up to 1% and a time precision of up to 14 ps (sigma). Together with an elaborate particle-tracking system, the full identification of relativistic ions from hydrogen up to uranium in mass and nuclear charge is possible.
    Type of Medium: Online Resource
    ISSN: 1434-601X
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 1413603-X
    detail.hit.zdb_id: 1459066-9
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  • 3
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2022
    In:  Journal für Urologie und Urogynäkologie/Österreich Vol. 29, No. 2 ( 2022-06), p. 46-53
    In: Journal für Urologie und Urogynäkologie/Österreich, Springer Science and Business Media LLC, Vol. 29, No. 2 ( 2022-06), p. 46-53
    Abstract: Male urethral stricture is a pathologic narrowing of the anterior urethra. It is a common urologic disease and, in the Western world, mostly iatrogenic or idiopathic in nature. On validated questionnaires, which should be given out pre- and postoperatively, patients usually report a significant negative impact on their quality of life. Surgical treatment depends on etiology, location, length and texture of the stricture, as well as previous surgeries and patient preferences. Treatment options are numerous and sometimes complex. Thus, an experienced plastic reconstructive surgeon at a specialist center is required. Endourologic procedures include urethral dilatation and direct vision internal urethrotomy, which are indicated in primary short segment bulbar urethral strictures or for sail-shaped strictures without spongiofibrosis. If the patient’s general condition is good, longer ( 〉  1 cm), multilocular, complex, recurring or penile urethral strictures should be evaluated for open urethral reconstruction. Open surgical techniques include end-to-end anastomosis, substitution urethroplasty using an (oral mucosa) graft and reconstruction with a split-thickness mesh graft or as a pedicled flap repair. Perineal urethrostomy is an alternative surgery for patients with complex penile urethral strictures and is particularly suitable for frail patients that cannot or do not wish to undergo (additional) open urethral reconstruction.
    Type of Medium: Online Resource
    ISSN: 1023-6090 , 1680-9424
    Language: German
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2051806-7
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  • 4
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 191, No. 14 ( 2009-07-15), p. 4695-4695
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    American Society for Microbiology ; 2013
    In:  Journal of Clinical Microbiology Vol. 51, No. 1 ( 2013-01), p. 212-216
    In: Journal of Clinical Microbiology, American Society for Microbiology, Vol. 51, No. 1 ( 2013-01), p. 212-216
    Abstract: Since 1995, a methicillin-resistant Staphylococcus aureus (MRSA) clone has spread in southern Germany. The strain was assigned to the Rhine-Hesse pulsed-field gel electrophoresis (PFGE) type by the staphylococcal reference center and was highly similar to epidemic clones known to belong to clonal complex 5 (CC5; USA100) based on multilocus sequence typing (MLST). Here we analyzed a defined collection of strains assigned to the Rhine-Hesse/USA100 PFGE type. Using sequence-based typing methods (MLST, spa ), the isolates were divided into two distinct clusters, ST5 and its single-locus variant ST225. These two lineages are not distinguishable by PFGE or phage typing. Most of the ST5 isolates were derived from patients and volunteers from the Tübingen area in southwest Germany, whereas the ST225 isolates were mostly from other locations in Germany. The locally restricted ST5 isolates were shown to contain different SSC mec islands and exhibited different antibiotic resistance profiles. In contrast, the ST225 isolates form a highly homogenous group and are emerging all over Germany. The two lineages are clearly distinguishable by their phage content and spa type: ST5 strains from Tübingen are characterized by a Sa7int phage that carries the virulence gene sak , which codes for staphylokinase, and ST225 isolates are characterized by a Sa1int phage. In conclusion, based on sequence typing and phage content, CC5 strains can be subdivided into two distinct lineages with different epidemicities.
    Type of Medium: Online Resource
    ISSN: 0095-1137 , 1098-660X
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2013
    detail.hit.zdb_id: 1498353-9
    SSG: 12
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