In:
The FASEB Journal, Wiley, Vol. 24, No. S1 ( 2010-04)
Abstract:
Transcription factor induced reprogramming of somatic cells to induced pluripotent stem (iPS) cells, that are molecularly and functionally similar to ES cells, can be achieved by the expression of Oct4, Sox2, Klf4 and cMyc. We study the mechanism of the remarkable changes that accompany this process, including resetting of the somatic cell transcriptome, epigenome and, in female cells, the reactivation of the silent X‐chromosome. In a recent study, we have found that Oct4, Sox2, Klf4, and cMyc cooperate in binding to activate the embryonic housekeeping program, followed by the specific binding of Oct4, Sox2 and Klf4 to genes encoding pluripotency regulators leading to the self‐sustained, pluripotent state. Thus there are distinct temporal steps to the resetting of the transcription program. Currently, we are investigating the epigenetic roadblocks that impede the conversion of partially reprogrammed cells to complete pluripotency. Grant Funding Source : CIRM
Type of Medium:
Online Resource
ISSN:
0892-6638
,
1530-6860
DOI:
10.1096/fasebj.24.1_supplement.172.6
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1468876-1
detail.hit.zdb_id:
639186-2
SSG:
12
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