In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 11 ( 2021-11-4), p. e3001284-
Abstract:
The emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a pandemic causing significant damage to public health and the economy. Efforts to understand the mechanisms of Coronavirus Disease 2019 (COVID-19) have been hampered by the lack of robust mouse models. To overcome this barrier, we used a reverse genetic system to generate a mouse-adapted strain of SARS-CoV-2. Incorporating key mutations found in SARS-CoV-2 variants, this model recapitulates critical elements of human infection including viral replication in the lung, immune cell infiltration, and significant in vivo disease. Importantly, mouse adaptation of SARS-CoV-2 does not impair replication in human airway cells and maintains antigenicity similar to human SARS-CoV-2 strains. Coupled with the incorporation of mutations found in variants of concern, CMA3p20 offers several advantages over other mouse-adapted SARS-CoV-2 strains. Using this model, we demonstrate that SARS-CoV-2–infected mice are protected from lethal challenge with the original Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), suggesting immunity from heterologous Coronavirus (CoV) strains. Together, the results highlight the use of this mouse model for further study of SARS-CoV-2 infection and disease.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001284
DOI:
10.1371/journal.pbio.3001284.g001
DOI:
10.1371/journal.pbio.3001284.g002
DOI:
10.1371/journal.pbio.3001284.g003
DOI:
10.1371/journal.pbio.3001284.g004
DOI:
10.1371/journal.pbio.3001284.s001
DOI:
10.1371/journal.pbio.3001284.s002
DOI:
10.1371/journal.pbio.3001284.s003
DOI:
10.1371/journal.pbio.3001284.s004
DOI:
10.1371/journal.pbio.3001284.s005
DOI:
10.1371/journal.pbio.3001284.s006
DOI:
10.1371/journal.pbio.3001284.s007
DOI:
10.1371/journal.pbio.3001284.s008
DOI:
10.1371/journal.pbio.3001284.s009
DOI:
10.1371/journal.pbio.3001284.s010
DOI:
10.1371/journal.pbio.3001284.s011
DOI:
10.1371/journal.pbio.3001284.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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