In:
Fundamental & Clinical Pharmacology, Wiley, Vol. 32, No. 5 ( 2018-10), p. 507-515
Abstract:
Epoxy‐carvone ( EC ) has chiral centers that allow generation of stereoisomers, including (+)‐cis‐ EC and (−)‐cis‐ EC , whose effects in the kindling tests have never been studied. Accordingly, this study aims to comparatively investigate the effect of stereoisomers (+)‐cis‐epoxy‐carvone and (−)‐cis‐epoxy‐carvone on behavioral changes measured in scores, in the levels of cytokines ( IL ‐1β, IL ‐6, and TNF α) and neuronal protection in the face of continuous treatment with pentylenetetrazol. Swiss mice were divided into five groups ( n = 10), receiving vehicle, (+) – cis‐ EC , (−) – cis‐ EC (both at the dose of 30 mg/kg), and diazepam (4 mg/kg). Thirty minutes after the respective treatment was administered to the animals one subconvulsive dose of PTZ (35 mg/kg). Seven subconvulsives treatments were made on alternate days, in which each treatment several parameters were recorded. In the eighth treatment, the animals receiving the highest dose of PTZ (75 mg/kg) and were sacrificed for quantification of cytokines and histopathologic analysis. All drugs were administered by intraperitoneal route. In the kindling test, (+)‐cis‐ EC and (−)‐cis‐ EC reduced the average scores. The stereoisomer (+)‐cis‐ EC decreased levels of proinflammatory cytokines IL ‐1β, IL ‐6, and TNF α, whereas comparatively (−)‐cis‐ EC did not reduce IL ‐1β levels. Histopathological analysis of the mice hippocampi undergoing this methodology showed neural protection for treated with (+)‐cis‐ EC . The results suggest that the anticonvulsant effect of (+)‐cis‐ EC possibly takes place due to reduction of proinflammatory cytokines involved in the epileptogenic process, besides neuronal protection, yet further investigation of the mechanisms involved is required.
Type of Medium:
Online Resource
ISSN:
0767-3981
,
1472-8206
DOI:
10.1111/fcp.2018.32.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2006242-4
SSG:
15,3
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