In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 59, No. 2 ( 1996-02-01), p. 219-228
Abstract:
Alkyl-PAF induced two components of polymorphonuclear leukocyte (PMN) pruning for enhanced fMLP- and rC5a-induced superoxide anion (O2−) production. Component A priming had a shallow, linear alkyl-PAF concentration-response slope (10 pM–1 nM), and component B priming had a significantly steeper concentration-response slope (1–100 nM alkyl-PAF). Whereas the extent of component B priming decayed significantly within 5–10 min after pretreatment of PMNs with alkyl-PAF, component A priming was completely stable. WEB 2086, a specific and potent PAF receptor antagonist, abolished component A pruning when PMNs were simultaneously stimulated with alkyl-PAF and either fMLP or rC5a but only partially reduced component B priming. However, whereas WEB 2086 also obliterated component A priming when PMNs were pre-treated with alkyl-PAF for 2.5, 5, or 10 min prior to fMLP stimulation, WEB 2086 had little or no inhibitory effect on component B priming. Paradoxically, WEB 2086 significantly augmented alkyl-PAF–induced component B priming for enhanced rC5a-induced PMN O2− production yet concomitantly obliterated component A printing. PMN pruning by acyl-PAF (1 nM–1 μM) had characteristics identical to those of alkyi-PAF–induced component A priming. These studies suggest that there are at least two effector pathways modulating alkyl-PAF–induced PMN respiratory burst priming. They are also consistent with the notion that component A priming is initiated via high-affinity PAF receptors and component B priming is mediated through low-affinity PAF receptors; and whereas alkyl-PAF interacts with both high- and low-affinity PAF receptors, both acyl-PAF and WEB 2086 preferentially bind to the high-affinity PAF receptors.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.59.2.219
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1996
detail.hit.zdb_id:
2026833-6
SSG:
12
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