In:
Cytoskeleton, Wiley, Vol. 70, No. 6 ( 2013-06), p. 328-348
Abstract:
Myosins comprise a highly conserved superfamily of eukaryotic actin‐dependent motor proteins implicated in a large repertoire of functions in both the cytoplasm and the nucleus. Class XVI myosin, MYO16, reveals expression in most somatic as well as meiotic cells with prominent localization in the nucleus, excepting the nucleolus; however, the role(s) of Myo16 in the nucleus remain unknown. In this report, we investigated Myo16 abundance during transit through the cell cycle. Immunolocalization, immunoblot, flow cytometric and quantitative RT‐PCR studies performed in Rat2 cells indicate that Myo16 mRNA and protein abundance are cell cycle regulated: in the unperturbed cell cycle, each rises to peak levels in late G 1 and thereon through S‐phase and each decays as cells enter M‐phase. Notably, RNA interference‐induced Myo16 depletion results in altered cell cycle distribution as well as in large‐scale cell death. In response to DNA replication stress (impaired replication fork progression as a consequence of DNA damage, lack of sufficient deoxynucleotides, or inhibition of DNA polymerases), Myo16 protein shows substantial loss. Attenuation of replication stress (aphidicolin or hydroxyurea) is followed by a recovery of Myo16 expression and resumption of S‐phase progression. Collectively, these observations suggest that Myo16 may play a regulatory role in cell cycle progression. © 2013 Wiley Periodicals, Inc.
Type of Medium:
Online Resource
ISSN:
1949-3584
,
1949-3592
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2536522-8
SSG:
12
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