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  • 1
    Online Resource
    Online Resource
    Draft Genome Sequence of Streptomyces cavourensis YBQ59, an Endophytic Producer of Antibiotics Bafilomycin D, Nonactic Acid, Prelactone B, and 5,11-Epoxy-10-Cadinanol
    American Society for Microbiology ; 2018
    In:  Microbiology Resource Announcements Vol. 7, No. 11 ( 2018-09-20)
    Details
    In: Microbiology Resource Announcements, American Society for Microbiology, Vol. 7, No. 11 ( 2018-09-20)
    Abstract: This study reports the draft genome sequence of the endophytic Streptomyces cavourensis strain YBQ59, produces the antibiotics bafilomycin D, nonactic acid, prelactone B, and 5,11-epoxy-10-cadinanol. The draft genome sequence comprises ∼10.2 Mb, with a GC content of 64% and 8,958 predicted protein-coding genes, of which 14 gene clusters were found to associate with antibiotic biosynthetic pathways.
    Type of Medium: Online Resource
    ISSN: 2576-098X
    URL: Article
    DOI: 10.1128/MRA.01056-18
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2018
    detail.hit.zdb_id: 2968655-6
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  • 2
    Online Resource
    Online Resource
    Compressor disc structural optimization for gas turbine engine
    IOP Publishing ; 2020
    In:  IOP Conference Series: Materials Science and Engineering Vol. 862, No. 2 ( 2020-05-01), p. 022017-
    Details
    In: IOP Conference Series: Materials Science and Engineering, IOP Publishing, Vol. 862, No. 2 ( 2020-05-01), p. 022017-
    Abstract: Gas turbine engine compressor increases the pressure and temperature of the air stream through it before entering the combustion chamber. Due to the extreme in rotational speed and high temperature, the gas turbine engine compressor is subjected to a complex force system that causes different failures mode in the compressor disc. Therefore, the design and calculation of compressor discs always consist of very high requirements with the problem of reducing the volume and increasing the durability of the disc structure. This paper presents a computational method that optimizes the design of a compressor disc geometry that meets performance and durability parameters but has the lowest mass based on finite element method. The calculation program is implemented by software Abaqus 6.13, algorithms and analysis results are processed through the Python 3 programming language. A post-publication change was made to this article on 11 Jun 2020 to correct the pdf so that it matched the webpage.
    Type of Medium: Online Resource
    ISSN: 1757-8981 , 1757-899X
    URL: Article
    DOI: 10.1088/1757-899X/862/2/022017
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2020
    detail.hit.zdb_id: 2506501-4
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  • 3
    Online Resource
    Online Resource
    Genome Mining Reveals the Biosynthetic Pathways of Polyhydroxyalkanoate and Ectoines of the Halophilic Strain Salinivibrio proteolyticus M318 Isolated from Fermented Shrimp Paste
    Springer Science and Business Media LLC ; 2020
    In:  Marine Biotechnology Vol. 22, No. 5 ( 2020-10), p. 651-660
    Details
    In: Marine Biotechnology, Springer Science and Business Media LLC, Vol. 22, No. 5 ( 2020-10), p. 651-660
    Type of Medium: Online Resource
    ISSN: 1436-2228 , 1436-2236
    URL: Article
    DOI: 10.1007/s10126-020-09986-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 1479877-3
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Effect of cultural conditions on antimicrobial activity of marine-derived fungus Penicillium chrysogenum
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 4 ( 2018-04-19), p. 727-733
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 4 ( 2018-04-19), p. 727-733
    Abstract: Marine fungi are a potential source of structurally diversified bioactive secondary metabolites that are not found in terrestrial sources. In our continuous investigation to search new antimicrobial agents from marine-derived fungi, we isolated fungal strain 045-357-2 from a soft coral sample collected from Ca Na bay, Ninh Thuan, Vietnam. The strain showed high antibacterial activity and was selected for further study. According to Internal Transcribed Spacer (ITS) rDNA molecular methods, the fungus was identified as Penicillium chrysogenum 045-357-2 and had a 100% homology (600/600 bp) with a GenBank sequence from a reference P. chrysogenum strain (NCBI accession no. EF200090). In the present investigation, the fungus was studied on effect of different culture incubation period, pH and salinity for antimicrobial activity against pathogenic microbes including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Streptococcus faecalis, Listeria monocytogenes, and Candida albicans. The ethyl acetate extracts of culture medium of the fungal isolate were determined antimicrobial activity by disc diffusion assay. The results showed that the optimum antimicrobial activity of the fungal strain P. chrysogenum 045-357-2 was obtained in medium containing 20 g rice, 20 mg yeast extract, 10 mg KH2PO4 , and 40 mL natural seawater at 30oC on the 14th day of incubation. The strain also exhibited highest antimicrobial activity in the medium at initial pH 6.0 – 8.0 and salinity of 35 g/L. The findings indicate that cultural conditions significantly influenced on antimicrobial activity of the studied fungus. The optimization is potential use for further study on the mass cultivation and isolation of bioactive compounds for this fungus.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/14/4/12307
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 5
    Online Resource
    Online Resource
    Isolation and screening marine fungi with antimicrobial activity from samples collected in Nha Trang bay, VietNam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 1 ( 2018-12-17), p. 181-187
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 1 ( 2018-12-17), p. 181-187
    Abstract: The marine environment is an extremely complex ecosystem and contains a broad spectrum of fungal diversity. Marine fungi have been shown to be tremendous sources for new and biologically active secondary metabolites. The present study aims to isolate and screen antimicrobial properties of 100 fungus strains from different marine sources including seaweeds, soft corals, sponges and sediment collected at Nha Trang Bay, Vietnam. In preliminary experiments, the crude extracts of these fungal isolates with ethyl acetate were screened for their antimicrobial activity against the human microbial pathogens including Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC 19111, Streptococcus faecalis ATCC 19433 and Candida albicans ATCC 10231 by the disc diffusion method. Among the 100 isolates, 59 strains exhibited antimicrobial activity against at least two tested pathogens, that 57% against S. aureus, 50% against L. monocytogenes, 49% against B. cereus, 45% against S. faecalis, 7% against E. coli, 5% against C. albicans, and only 2% against P. aeruginosa. The present study has revealed the presence of high numbers of marine fungi from Nha Trang waters having antimicrobial properties and they need to be investigated further for natural bioactive products.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/16/1/13463
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 6
    Online Resource
    Online Resource
    EFFECT OF CULTURE CONDITIONS FOR ANTIMICROBIAL ACTIVITY OF MARINE - DERIVED FUNGUS ASPERGILLUS FLOCCULOSUS 01NT.1.1.5
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 15, No. 4 ( 2018-12-14), p. 721-728
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 15, No. 4 ( 2018-12-14), p. 721-728
    Abstract: The biosynthesis of compounds with antibiotic activity produced by marine fungi, strongly depends on their growth conditions. A good understanding of the role of culture conditions in the biosynthesis of metabolites may lead to better exploitation of microbial metabolites. In this study, the influence of culture conditions including incubation period, initial pH and salinity on antimicrobial activity and secondary metabolites production of marine fungus 01NT.1.1.5 was investigated. This isolate, obtained from sponge Stylissa sp. in Nha Trang Bay, exhibited a broad spectrum of in vitro antimicrobial activity to Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 19111, Streptococcus faecalis ATCC 19433 and Candida albicans ATCC 10231. According to morphological characteristics and sequence analysis of 28S rDNA, the fungus was identified as Aspergillus flocculosus. The results indicated that antimicrobial activity and metabolite amount were highest when the fungus was cultivated in rice medium with incubation period of 20 days. The optimum salinity of 35 g/L and initial pH of 6.0 were found for the maximum antibiotic production. The colony growth, antimicrobial activity and production of secondary metabolites of the strain A. flocculosus 01NT.1.1.5 varied depending on salt concentrations and initial pH of medium. Particularly, extract of this fungus only showed activity against C. albicans when it was cultured in medium with 30-35 g/L salinity and initial pH 4.0-8.0. The results  indicate that salinity and initial pH along with cultivation period are important factors influencing antimicrobial activity and secondary metabolites of A. flocculosus 01NT.1.1.5, and might be for other marine fungi.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15/4/13415
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 7
    Online Resource
    Online Resource
    Isolation and screening of endophytic bacteria from Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  TAP CHI SINH HOC Vol. 40, No. 2 ( 2018-11-06), p. 153-161
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 40, No. 2 ( 2018-11-06), p. 153-161
    Abstract: Biotransformation of secondary metabolites from medicinal plants by bacteria has been studied extensively in the recent years. One of the potential sources for screening bacteria is plant endophytic bacteria. In this study, we isolated and screened endophytic bacteria for transformation of ginsenoside Rb1 of Ngoc Linh ginseng collected at village number 2, Tra Linh commune, Nam Tra My district, Quang Nam province. There are 45 strains isolated form rhizobium (24 strains), petioles (8 strains) and leaves (13 strains). After screening, there are 27 strains positive with β-glucosidase test, an enzyme which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides-aglycone linkage of ginsenoside Rb. By evaluating the β-glucosidase activity and identification via 16S rRNA sequence, we choosed four high β-glucosidase acitivity strains SVK32 (Enterobacter sp.); SVK34 (Serratia sp.); SVK37 (Ochrobactrum sp.) and SVK44 (Arthrobacter sp.) for futher study on biotransformating of ginsenoside Rb1 into ginsenoside Rd and Rg3.
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v40n2.10862
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 8
    Online Resource
    Online Resource
    Antagonic Activity of Endophytic Bacteria Isolated from Man Trau Grass on Stem Rot Disease Of Pitaya (Hylocereus Undatus)
    Vietnam National University Journal of Science ; 2021
    In:  VNU Journal of Science: Natural Sciences and Technology Vol. 37, No. 2 ( 2021-06-28)
    Details
    In: VNU Journal of Science: Natural Sciences and Technology, Vietnam National University Journal of Science, Vol. 37, No. 2 ( 2021-06-28)
    Abstract: Endophytic bacteria reside in the intercellular spaces in different plant tissues without causing damage. This study aimed to select endophytic bacterial strains from Man Trau grass (Eleusine indica) that are able to inbibit the fungus A. alternata  causing stem rot disease on pitaya. The result showed that one fungal isolate was pathogenic and caused stem rot disease on dragonfruit. The analysis results based on morphological characteristics and ITS region sequence indicated that the isolated fungal strain is 100% identical to A. alternata. In addition, a total of 16 endophytic bacterial strains were isolated from Man Trau grass samples in Binh Thuan and screened for antifungal activity. Ten bacterial strains with antagonistic activity against A. alternata causing stem rot disease on pitaya were detected. Among those, strain MT47 showed the strongest antifungal activity with an efficiency of 36.67%. Moreover, the result also presented that the antagonistic strain MT47 pocesses the ability of biofilm formation significantly higher than the control. The results suggest that these endophytic bacteria can be used in biocontrol of the stem rot disease on pitaya.
    Type of Medium: Online Resource
    ISSN: 2588-1140 , 2615-9317
    URL: Article
    DOI: 10.25073/2588-1140/vnunst.5125
    Language: Unknown
    Publisher: Vietnam National University Journal of Science
    Publication Date: 2021
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  • 9
    Online Resource
    Online Resource
    Classification and characterization of endophytic actinomycetes isolated from cinnamomum cassia presl
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 1 ( 2018-12-17), p. 149-155
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 1 ( 2018-12-17), p. 149-155
    Abstract: Currently, antibiotic resistance in pathogenic bacteria is a significant clinical problem with the increase of deseases and a serious public health concern. Thus, the identification of new antimicrobial agents, especially the secondary metabolites products by endophytic actinobacteria from medical plants could be promising sources of biologically active compounds in medical fields. This study focused on identification and evaluation of antimicrobial activity against pathogens; genes involved in their secondary metabolisms, and screening of anthracycline producing capacity (mainly presented in anti-cancer antibiotics) of YBQ75 isolated from Cinnamomum cassia Presl. plants in Yen Bai province. Based on manual of bacterial classification, method in International Streptomyces Project (ISP) and the 16S rRNA gene sequence (GenBank Acc. No. KR814822), the endophytic actinomycetes YBQ75 was named Streptomyces cavourensis YBQ75 with 100% identity. The strain S. cavourensis YBQ75 showed the remarkable antibacterial activities against 5 tested pathogens (Salmonella enterica ATCC 14028 (22.0 mm); Pseudomonas aeruginosa CNLM (19.3 mm); Staphylococcus epidermidis ATCC 12228 (19.3 mm); Enterobacter aerogenes ATCC 13048 (17.7 mm) and Proteus vulgaris CNLM (16.3 mm)) in the total of 9 tested pathogens. The detection of genes involved in antibiotic synthesis indicated that the strain S. cavourensis YBQ75 consists of all three genes related to antibiotic synthesis including polyketide synthase (pks-I) type I, polyketide synthase type II (pks-II) and nonribosomal peptide synthetase (nrps). Premarilly result showed that the strain S. cavourensis YBQ75 also present as an anthracycline productive actinomycetes. The resutls demonstrated that the endophytic actinomycetes S. cavourensis YBQ75 from medical plants could be promising sources for the production of antibiotics and anthracycline anticancer compounds.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/16/1/13459
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 10
    Online Resource
    Online Resource
    ENZYMATIC SACHARIFICATION OF ALKALINE PRETREATED RICE STRAW BY CELLULASE FROM CELLULOSIMICROBIUM SP. MP1
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Science and Technology Vol. 58, No. 6A ( 2021-03-31), p. 244-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 58, No. 6A ( 2021-03-31), p. 244-
    Abstract: The effects of different physical and technological parameter such as time, substrate to liquid ratio, enzyme concentration, temperature, and pH on enzymatic saccharification of alkaline pretreated straw cellulose were studied. For alkaline pretreatment, the straw was incubated with 10 % NaOH at ratio 1:20 (w/v) at 90 °C for 1 hour. After the alkaline pretreatment the cellulose content increased from 50.2 % (w/w) to 67.3 % (w/w). Enzyme used for saccharification of treated and untreated straw  was produced from Cellulosimicrobium sp MP1 which was isolated from termite gut. Results from research showed that the highest percentage of saccharification of alkaline pretreated straw was 69.91 %, corresponding to 10.58 mg/mL of reducing sugar. The hydrolysis conditions for reaching this highest saccharification yield were: temperature of 55 ºC, substrate to liquid ratio of 2 g/100 mL, enzyme concentration of 37.5 U/g, pH of 5.5 and hydrolysis time of 48 hours.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/58/6A/15579
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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