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  • 1
    Online Resource
    Online Resource
    Combined effect of methotrexate and bifidobacteria metabolites on TNFα AND IFNγ production by human peripheral blood mononuclears
    Russian Society of Immunology ; 2023
    In:  Russian Journal of Immunology Vol. 26, No. 3 ( 2023-08-11), p. 281-286
    Details
    In: Russian Journal of Immunology, Russian Society of Immunology, Vol. 26, No. 3 ( 2023-08-11), p. 281-286
    Abstract: Methotrexate (Mtx) is a first-line drug for the treatment of numerous rheumatic and non-rheumatic disorders, including oncological disdiseases. However, therapeutic efficacy of Mtx is limited by severe toxicity to many organs (myelo-, hepato-, nephrotoxicity, mucositis, enteritis, dysbiosis at various human biotopes, etc.). Recently, a number of studies showed that some metabolites of Bifidobacteria and Lactobacilli are able to enhance effect of chemotherapeutic drugs and limit their toxic properties. The aim of the present work was to study the possible potentiating action of Bifidobacteria cell-free supernatants and methotrexate upon secretion of pro-inflammatory TNF and IFN cytokines by human peripheral blood mononuclear cells (PBMCs). The immunoregulatory effects upon production of TNF and IFNg was evaluated in the in vitro model of cultured PBMC supplemented with Bifidobacteria metabolites, methotrexate, or their combination. Analysis of the combined effect of Bifidobacteria metabolites and Mtx on the cytokine production revealed their synergism towards the key pro-inflammatory cytokines (TNF and IFN). We found an increase against the control cultures (with Mtx only), inhibition of the early pro-inflammatory cytokine TNF production. On the contrary, we revealed an increased secretion of IFN which regulates the effector cells. The results obtained with these cytokines suggest the presence of a potentiating effect of Bifidobacteria metabolites upon anti-inflammatory and immunoregulatory properties of methotrexate. Thus, Bifidobacteria metabolites can be considered a promising agent which potentiates the therapeutic action of methotrexate by suppressing TNF secretion and stimulating IFN by immunocompetent cells. Further studies of the combined effects of Mtx and metabolites from the intestinal microbiota upon the cytokine production by effector cells could be recommended, aiming to enhance therapeutic effect of methotrexate and limit its toxic properties using the Bifidobacteria metabolites.
    Type of Medium: Online Resource
    ISSN: 2782-7291 , 1028-7221
    URL: Article
    DOI: 10.46235/1028-7221-9839-CEO
    Language: Unknown
    Publisher: Russian Society of Immunology
    Publication Date: 2023
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  • 2
    Online Resource
    Online Resource
    Рис.1c
    ECO-Vector LLC ; 2023
    In:  Morphology Vol. 160, No. 1 ( 2023-02-11), p. 5-10
    Details
    In: Morphology, ECO-Vector LLC, Vol. 160, No. 1 ( 2023-02-11), p. 5-10
    Abstract: MATERIAL AND METHODS: This study was carried out on 20 male Wistar rats, of which 15 were injected with a bacterialfungal suspension into the cavity of the hock (tarsus) joint. Specifically, the rats were injected with KlebsiellaCandida suspension (1:1) with a microbial concentration of 106 CFU/ml prepared by using the museum strains Klebsiella pneumoniae ICIS-278 and Candida albicans ATCC 24433 under the control of an X-ray apparatus and digitizer FireCR+. Five intact animals served as the control. On day 15 of the experiment, a bacteriological and histological examination of the affected joints was carried out while maintaining topography after euthanasia. RESULTS: In rats infected with the KlebsiellaCandidiasis suspension, pain syndrome and functional changes typical of arthritis were noted on days 23. The bacteriological examination of the affected joints in 75% of rats isolated K. pneumonia cultures that were identical, according to polymerase chain reaction, to the original strain that was part of the bacterialfungal suspension. At the same time, fungi of the genus Candida, which was also used in the experiment to infect animals, were not detected in the affected joints of rats. The established model of KlebsiellaCandida arthritis was characterized by the pathomorphological signs of panarthritis accompanied by the purulent inflammation of all joint structures (synovial membrane, cartilage, and bone tissue) with the involvement of soft periarticular tissues. CONCLUSION: The model of Klebsiella and fungal arthritis presented by this work can be used to test new means of antimicrobial (antibacterial and antifungal) therapies, which are an integral component of the treatment of infection-associated arthritis.
    Type of Medium: Online Resource
    ISSN: 1026-3543
    URL: Issue
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.17816/morph.20221601
    DOI: 10.17816/morph.110871
    DOI: 10.17816/morph.110871-70502
    DOI: 10.17816/morph.110871-70503
    DOI: 10.17816/morph.110871-70504
    Language: Unknown
    Publisher: ECO-Vector LLC
    Publication Date: 2023
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  • 3
    Online Resource
    Online Resource
    Covid and Oxytocin: Looking for Microbial Symbiosis
    University Hospitals of Morecambe Bay NHS Foundation Trust ; 2021
    In:  Morecambe Bay Medical Journal Vol. 8, No. 10 ( 2021-08-01), p. 279-283
    Details
    In: Morecambe Bay Medical Journal, University Hospitals of Morecambe Bay NHS Foundation Trust, Vol. 8, No. 10 ( 2021-08-01), p. 279-283
    Abstract: Oxytocin is a hormone with broad implications for general health. This hormone has anti-inflammatory and antioxidant protective effects and has received particular attention due to the pandemic of COVID-19. This review examines materials on the role of microbial symbiosis in COVID-19 and the effect of microbiota on oxytocin. It opens new potential prospects for the use of microbiota and new “nature-like” technologies.
    Type of Medium: Online Resource
    ISSN: 2634-0631 , 0957-5286
    URL: Article
    DOI: 10.48037/mbmj.v8i10.1312
    Language: Unknown
    Publisher: University Hospitals of Morecambe Bay NHS Foundation Trust
    Publication Date: 2021
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