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  • 1
    Online Resource
    Online Resource
    NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics
    Wiley ; 2020
    In:  Ecology Vol. 101, No. 11 ( 2020-11)
    Details
    In: Ecology, Wiley, Vol. 101, No. 11 ( 2020-11)
    Abstract: Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal‐central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus , Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. ( n = 37,782), Sus scrofa ( n = 6,730), and Canis familiaris ( n = 10,084); 17 species were represented by only one record (e.g., S yncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans ). Primates have the highest number of species in the data set ( n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata , and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation‐related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data.
    Type of Medium: Online Resource
    ISSN: 0012-9658 , 1939-9170
    URL: Issue
    URL: Article
    DOI: 10.1002/ecy.v101.11
    DOI: 10.1002/ecy.3115
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1797-8
    detail.hit.zdb_id: 2010140-5
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    The oil-resin of the tropical rainforest tree Copaifera langsdorffii reduces cell viability, changes cell morphology and induces cell death in human endometriotic stromal cultures
    Oxford University Press (OUP) ; 2015
    In:  Journal of Pharmacy and Pharmacology Vol. 67, No. 12 ( 2015-12-15), p. 1744-1755
    Details
    In: Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 67, No. 12 ( 2015-12-15), p. 1744-1755
    Abstract: The hormonal treatment for endometriosis frequently fails to completely eradicate endometriotic implants. A new therapeutic treatment is needed. This study investigates the in-vitro effect of Copaifera langsdorffii oil-resin on human eutopic and ectopic endometrium stromal cell cultures (EuESCs and EctESCs). Methods A nanocomposite system containing the copaiba oil-resin (NanoCOR) was developed and acute toxicity test was performed. Endometrial stromal cells (ESCs) from non-endometriotics controls (CESCs), EuESCs and EctESCs were isolated and treated with different concentrations of NanoCOR, at different time intervals to evaluate its effect on cell morphology, proliferation, viability, necrosis and apoptosis induction. Key findings When treated with 50 μg/ml of NanoCOR, the morphology of EctESCs changed, as the actin microfilaments were disorganized, disassembled or disrupted. Moreover, at 24 h of treatment with NanoCOR, the EctESCs viability was inhibited, and a significant number of these cells underwent apoptosis. In EuESCs, these effects were observed only at 48 h. Finally, the treatment of EctESCs with NanoCOR increased the lactate dehydrogenase release into the extracellular medium more than in EuESCs. Conclusions Our data indicate that NanoCOR has a greater impact on the behaviour of human endometriotic stromal cells than on the eutopic endometrium stromal cells, supporting the idea that NanoCOR should be further investigated as a novel and valuable alternative to treat endometriosis.
    Type of Medium: Online Resource
    ISSN: 2042-7158 , 0022-3573
    URL: Article
    DOI: 10.1111/jphp.12479
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2015
    detail.hit.zdb_id: 2041988-0
    detail.hit.zdb_id: 2050532-2
    SSG: 15,3
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  • 3
    Online Resource
    Online Resource
    Evaluation of the performance of two strains of Nile tilapia (Oreochromis Niloticus) in mixed raising systems
    FapUNIFESP (SciELO) ; 2008
    In:  Brazilian Archives of Biology and Technology Vol. 51, No. 3 ( 2008-06), p. 531-538
    Details
    In: Brazilian Archives of Biology and Technology, FapUNIFESP (SciELO), Vol. 51, No. 3 ( 2008-06), p. 531-538
    Abstract: O objetivo deste trabalho foi avaliar o desempenho produtivo de duas linhagens de tilápia do Nilo (Oreochromis niloticus) em sistemas de cultivo misto. Foram utilizados 3600 alevinos de tilápia, 1800 da linhagem Bouaké e 1800 da Chitralada. O experimento foi conduzido em três fases, a Fase I realizada em estufa em 18 caixas, nas quais foram testados dois tratamentos (Bouaké e Chitralada) e nove repetições; e a Fase II e III realizadas em 18 tanques de alvenaria, com os mesmos tratamentos. Na fase I, a linhagem Chitralada apresentou os maiores valores para peso final e ganho em peso. Na fase II, a Chitralada apresentou o maior valor para peso final em relação à Bouaké, já para o ganho em peso a Bouaké obteve o melhor resultado. Na fase III, a Chitralada apresentou os melhores resultados para peso final (104 dias de cultivo); peso final, comprimento final e ganho em comprimento (152 dias de cultivo) e aos 279 dias de cultivo, o ganho em peso e em comprimento foram maiores para a Bouaké. Estes resultados demonstraram que a linhagem de melhor desempenho foi a Chitralada.
    Type of Medium: Online Resource
    ISSN: 1516-8913
    URL: Article
    DOI: 10.1590/S1516-89132008000300012
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2008
    detail.hit.zdb_id: 2122231-9
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Injuries in pacu embryos ( Piaractus mesopotamicus ) after freezing and thawing
    Cambridge University Press (CUP) ; 2014
    In:  Zygote Vol. 22, No. 1 ( 2014-02), p. 25-31
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 22, No. 1 ( 2014-02), p. 25-31
    Abstract: Although the sperm cryopreservation of freshwater and marine teleosts has been feasible for years, the cryopreservation of some fish embryos still remains elusive. Thus, the objective of this experiment was to analyze the embryo morphology after freezing and thawing 40 embryos of Piaractus mesopotamicus immersed into methanol and ethylene glycol, both at 7, 10 and 13% plus 0.1 M sucrose for 10 min. Soon after thawing, three embryos were treated with historesin, stained with hematoxylin–eosin and analyzed under an optical microscope. From every treatment, one palette containing embryos was thawed and incubated, but none of the eggs hatched. Samples containing two embryos were immersed into 10% methanol or 10% ethylene glycol both in association with sucrose, and embryos immersed into only water or sucrose solution were frozen, processed and analyzed using scanning electron microscopy (SEM). In both cases, the control group was immersed into only water. Although the embryos had the chorion, vitello, yolk syncytial layer and blastoderm, all of them were found altered under the optical microscope and by SEM. The chorion was irregular and injured; there was no individuality in the yolk granules; the yolk syncytial layer had an irregular shape, thickness and size; the blastoderm showed injuries in the nucleus shape and sometimes was absent; the blastoderm was located in atypical areas and absent in some embryos. In conclusion, no treatment was effective in preserving the embryos, and none of the embryos avoided injury from intracellular ice formation. These morphological injuries during the freezing process made the P. mesopotamicus embryos unfeasible for hatching.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S096719941200024X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2014
    detail.hit.zdb_id: 1483381-5
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    DataSheet1.DOCX
    Frontiers Media SA ; 2024
    In:  Frontiers in Bioengineering and Biotechnology Vol. 11 ( 2024-1-4)
    Details
    In: Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 11 ( 2024-1-4)
    Abstract: Introduction: The use of chitosan in pharmaceutical formulations is an advantageous approach due to this compound intrinsic biodegradability and biocompatibility, as well as ready availability and low polymer cost. Methods: Herein, the naphthoquinones 3- chloromethylene-menadione (NQ1) and 2,3-dichloro-1,4-naphthoquinone (NQ2) were nanoencapsulated into chitosan (CNP) by the ionotropic gelatinization technique and characterized by DLS, FTIR, SEM, TGA and DSC, and their release profiles evaluated. The antimicrobial and wound healing activities were investigated. Results and Discussion: Homogeneous chitosan nanocapsulses of about 193 nm and Z potential ranging from +30.6 to +33.1 mV loaded with NQ1 (CNP-NQ1) or NQ2 (CNPQNQ2). With nanoencapsulation efficiencies of ≥ 96%, the solubility of naphthoquinones in aqueous environments was improved, making them suitable for biological system applications. The encapsulated naphthoquinones displayed a controlled release of approximately 80% for CNP-NQ1 and 90% for CNP-NQ2 over an 8 h period at 36°C. Both CNP-NQ1 and CNP-NQ2 retained the already established free naphthoquinone antimicrobial activity against two Staphylococcus aureus strains, Staphylococcus epidermidis , Streptococcus pyogenes and Pseudomonas aeruginosa . Although presenting low toxicity to healthy human cells, only CNP-NQ1 displayed therapeutic indices above 100 for S. aureus and S. epidermidis and above 27 for S. pyogenes and P. aeruginosa , allowing for safe use in human tissues. Furthermore, CNP-NQ1 did not impair the migration of human fibroblast cells in scratch assays, adding promising wound healing properties to this formulation. These findings emphasize that CNP-NQ1 may be useful in protecting injured skin tissue from bacterial contamination, avoiding skin infections not only by reducing bacterial loads but also by accelerating the healing process until complete dermal tissue recovery.
    Type of Medium: Online Resource
    ISSN: 2296-4185
    URL: Article
    URL: Article
    DOI: 10.3389/fbioe.2023.1284630
    DOI: 10.3389/fbioe.2023.1284630.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2024
    detail.hit.zdb_id: 2719493-0
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  • 6
    Online Resource
    Online Resource
    Effect of cryoprotectants on the survival of cascudo preto ( Rhinelepis aspera ) embryos stored at –8°C
    Cambridge University Press (CUP) ; 2014
    In:  Zygote Vol. 22, No. 1 ( 2014-02), p. 58-63
    Details
    In: Zygote, Cambridge University Press (CUP), Vol. 22, No. 1 ( 2014-02), p. 58-63
    Abstract: Cryopreservation of germplasm provides a promising method to preserve fish genetic material, which is of great importance in preservation of species diversity, aquaculture, and management of fish models used in biomedical research. In the present study, cryopreservation of Rhinelepis aspera embryos, a Brazilian endangered species, was studied for the first time using a short-term cooling protocol. Embryos at blastoporous closing stage were selected, placed in 6-ml glass vials and stored at −8°C for 6 h in 10 different cryoprotectant solutions: S1 (17.1% sucrose + 9% methanol); S2 (17.1% sucrose + 9% DMSO); S3 (8.5% sucrose + 8.5% glucose + 9% methanol); S4 (8.5% sucrose + 8.5% glucose + 9% DMSO); S5 (17.1% sucrose + 9% ethylene glycol); S6 (8.5% sucrose + 8.5% glucose + 9% ethylene glycol); S7 (17.1% sucrose + 4.5% methanol + 4.5% DMSO); S8 (17.1% sucrose + 4.5% methanol + 4.5% ethylene glycol); S9 (17.1% sucrose + 4.5% DMSO + 4.5% ethylene glycol); and S10 (100% water). Embryo viability was assessed by hatching rate, counting live larvae and number of failed eggs under a stereomicroscope. The results showed that only the cryoprotectant solutions that contained methanol associated to sucrose (S1, S7 and S8) provided partial protection of Rhinelepis aspera embryos from cold damage (over 50% hatching rate in S1), while the use of DMSO and ethylene glycol, isolated or in combination, resulted in no hatching rate. Further studies are needed in order to extend the storage time and to improve the hatching rate for the species.
    Type of Medium: Online Resource
    ISSN: 0967-1994 , 1469-8730
    URL: Article
    DOI: 10.1017/S0967199411000517
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2014
    detail.hit.zdb_id: 1483381-5
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Oral Iron Supplementation in Pregnancy: Current Recommendations and Evidence-Based Medicine
    Georg Thieme Verlag KG ; 2021
    In:  Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics Vol. 43, No. 10 ( 2021-10), p. 782-788
    Details
    In: Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics, Georg Thieme Verlag KG, Vol. 43, No. 10 ( 2021-10), p. 782-788
    Abstract: Objective To review the evidence about universal iron supplementation in pregnancy to prevent maternal anemia. Methods Bibliographic research of randomized and controlled clinical trials, meta-analyses, systematic reviews, and clinical guidelines, published between August 2009 and August 2019, using the MeSH terms: iron; therapeutic use; pregnancy; anemia, prevention and control. Results We included six clinical guidelines, three meta-analyses and one randomized controlled clinical trial. Discussion Most articles point to the improvement of hematological parameters and reduction of maternal anemia risk, with supplementary iron. However, they do not correlate this improvement in pregnant women without previous anemia with the eventual improvement of clinical parameters. Conclusion Universal iron supplementation in pregnancy is controversial, so we attribute a SORT C recommendation strength.
    Type of Medium: Online Resource
    ISSN: 0100-7203 , 1806-9339
    URL: Article
    DOI: 10.1055/s-0041-1736144
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2021
    detail.hit.zdb_id: 2105176-8
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  • 8
    Online Resource
    Online Resource
    Endometriosis: A Disease with Few Direct Treatment Options
    MDPI AG ; 2022
    In:  Molecules Vol. 27, No. 13 ( 2022-06-23), p. 4034-
    Details
    In: Molecules, MDPI AG, Vol. 27, No. 13 ( 2022-06-23), p. 4034-
    Abstract: Endometriosis is a gynecological condition characterized by the growth of endometrium-like tissues inside and outside the pelvic cavity. The evolution of the disease can lead to infertility in addition to high treatment costs. Currently, available medications are only effective in treating endometriosis-related pain; however, it is not a targeted treatment. The objective of this work is to review the characteristics of the disease, the diagnostic means and treatments available, as well as to discuss new therapeutic options.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules27134034
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 9
    Online Resource
    Online Resource
    Chitosans and Nanochitosans: Recent Advances in Skin Protection, Regeneration, and Repair
    MDPI AG ; 2022
    In:  Pharmaceutics Vol. 14, No. 6 ( 2022-06-20), p. 1307-
    Details
    In: Pharmaceutics, MDPI AG, Vol. 14, No. 6 ( 2022-06-20), p. 1307-
    Abstract: Chitosan displays a dual function, acting as both an active ingredient and/or carrier for pharmaceutical bioactive molecules and metal ions. Its hydroxyl- and amino-reactive groups and acetylation degree can be used to adjust this biopolymer’s physicochemical and pharmacological properties in different forms, including scaffolds, nanoparticles, fibers, sponges, films, and hydrogels, among others. In terms of pharmacological purposes, chitosan association with different polymers and the immobilization or entrapment of bioactive agents are effective strategies to achieve desired biological responses. Chitosan biocompatibility, water entrapment within nanofibrils, antioxidant character, and antimicrobial and anti-inflammatory properties, whether enhanced by other active components or not, ensure skin moisturization, as well as protection against bacteria colonization and oxidative imbalance. Chitosan-based nanomaterials can maintain or reconstruct skin architecture through topical or systemic delivery of hydrophilic or hydrophobic pharmaceuticals at controlled rates to treat skin affections, such as acne, inflammatory manifestations, wounds, or even tumorigenesis, by coating chemotherapy drugs. Herein, chitosan obtention, physicochemical characteristics, chemical modifications, and interactions with bioactive agents are presented and discussed. Molecular mechanisms involved in chitosan skin protection and recovery are highlighted by overlapping the events orchestrated by the signaling molecules secreted by different cell types to reconstitute healthy skin tissue structures and components.
    Type of Medium: Online Resource
    ISSN: 1999-4923
    URL: Article
    DOI: 10.3390/pharmaceutics14061307
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527217-2
    SSG: 15,3
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  • 10
    Online Resource
    Online Resource
    Phenylbenzohydrazides Obtained from Isatoic Anhydride Present Anti-Inflammatory Activity In Vivo and In Vitro
    MDPI AG ; 2022
    In:  Biomolecules Vol. 12, No. 12 ( 2022-12-19), p. 1901-
    Details
    In: Biomolecules, MDPI AG, Vol. 12, No. 12 ( 2022-12-19), p. 1901-
    Abstract: Background: Despite the existence of a wide variety of anti-inflammatory drugs, the vast majority are classified as steroidal or non-steroidal. Both classes present a variety of side effects that limit usage. Thus, the search for new molecules with anti-inflammatory potential is still important. Methods: Five phenylbenzohydrazides were synthetized and evaluated in pre-clinical models of acute inflammation in vivo and in vitro. Results: The new substances (INL-06, -07, -10, and -11), as well as AISCT, significantly reduced cell migration induced by carrageenan. It was also observed that all INLs inhibited protein extravasation as well as cytokines (IL-6, IL-1β, and TNF-α) and nitric oxide (NO) production. The INL-11 was demonstrated to be the most potent, since the inhibition observed in several parameters was significant even when compared with dexamethasone. In vitro INLs also reduced cytokines and NO production and inducible nitric oxide (iNOS) enzyme activity. The INL-11 was the most effective in reducing cell migration in vitro. Conclusions: Our data suggest that these substances are suitable for further development into a new series of compounds that could lead to new hits and future drug prototypes for anti-inflammatory conditions.
    Type of Medium: Online Resource
    ISSN: 2218-273X
    URL: Article
    DOI: 10.3390/biom12121901
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2701262-1
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