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  • 1
    Online Resource
    Online Resource
    Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography
    Bentham Science Publishers Ltd. ; 2019
    In:  Current Pharmaceutical Analysis Vol. 15, No. 5 ( 2019-05-23), p. 505-510
    Details
    In: Current Pharmaceutical Analysis, Bentham Science Publishers Ltd., Vol. 15, No. 5 ( 2019-05-23), p. 505-510
    Abstract: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol. Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation. Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.
    Type of Medium: Online Resource
    ISSN: 1573-4129
    URL: Article
    DOI: 10.2174/1573412914666180702150357
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2019
    SSG: 15,3
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  • 2
    Online Resource
    Online Resource
    Preparation and properties of the copper calcium titanate–modified poly(vinylidene fluoride)
    SAGE Publications ; 2015
    In:  Journal of Elastomers & Plastics Vol. 47, No. 5 ( 2015-08), p. 463-476
    Details
    In: Journal of Elastomers & Plastics, SAGE Publications, Vol. 47, No. 5 ( 2015-08), p. 463-476
    Abstract: This article described the preparation of copper calcium titanate (CCTO)-modified poly(vinylidene fluoride) (PVDF) by solution casting, and its dielectric properties, breakdown strength and tensile properties. The phase structure, crystal type and surface morphology of the membrane were determined using the methods of Fourier-transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and scanning electron microscopy. The results showed that the comprehensive performance of the CCTO/PVDF membrane treated with a coupling agent with a quality ratio of 2% was better than that of the membrane without coupling agent treatment. When the content of CCTO was increased to 20%, the surface structure of the membrane was compacted, and the membrane’s breakdown strength and dielectric properties were high.
    Type of Medium: Online Resource
    ISSN: 0095-2443 , 1530-8006
    URL: Article
    DOI: 10.1177/0095244314526735
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2098535-6
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  • 3
    Online Resource
    Online Resource
    Weighted Gene Co-Expression Analysis Network-Based Analysis on the Candidate Pathways and Hub Genes in Eggplant Bacterial Wilt-Resistance: A Plant Research Study
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 24 ( 2021-12-10), p. 13279-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 24 ( 2021-12-10), p. 13279-
    Abstract: Solanum melongena L. (eggplant) bacterial wilt is a severe soil borne disease. Here, this study aimed to explore the regulation mechanism of eggplant bacterial wilt-resistance by transcriptomics with weighted gene co-expression analysis network (WGCNA). The different expression genes (DEGs) of roots and stems were divided into 21 modules. The module of interest (root: indianred4, stem: coral3) with the highest correlation with the target traits was selected to elucidate resistance genes and pathways. The selected module of roots and stems co-enriched the pathways of MAPK signalling pathway, plant pathogen interaction, and glutathione metabolism. Each top 30 hub genes of the roots and stems co-enriched a large number of receptor kinase genes. A total of 14 interesting resistance-related genes were selected and verified with quantitative polymerase chain reaction (qPCR). The qPCR results were consistent with those of WGCNA. The hub gene of EGP00814 (namely SmRPP13L4) was further functionally verified; SmRPP13L4 positively regulated the resistance of eggplant to bacterial wilt by qPCR and virus-induced gene silencing (VIGS). Our study provides a reference for the interaction between eggplants and bacterial wilt and the breeding of broad-spectrum and specific eggplant varieties that are bacterial wilt-resistant.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms222413279
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Genome-wide capture sequencing to detect hepatitis C virus at the end of antiviral therapy
    Springer Science and Business Media LLC ; 2020
    In:  BMC Infectious Diseases Vol. 20, No. 1 ( 2020-12)
    Details
    In: BMC Infectious Diseases, Springer Science and Business Media LLC, Vol. 20, No. 1 ( 2020-12)
    Abstract: Viral relapse is a major concern in hepatitis C virus (HCV) antiviral therapy. Currently, there are no satisfactory methods to predict viral relapse, especially in the era of direct acting antivirals in which the virus often quickly becomes undetectable using PCR-based approaches that focus on a small viral region. Next-generation sequencing (NGS) provides an alternative option for viral detection in a genome-wide manner. However, owing to the overwhelming dominance of human genetic content in clinical specimens, direct detection of HCV by NGS has a low sensitivity and hence viral enrichment is required. Methods Based on template-dependent multiple displacement amplification (tdMDA), an improved method for whole genome amplification (Wang et al., 2017. Biotechniques 63, 21–27), we evaluated two strategies to enhance the sensitivity of NGS-based HCV detection: duplex-specific nuclease (DSN)-mediated depletion of human sequences and HCV probe-based capture sequencing. Results In DSN-mediated depletion, human sequences were significantly reduced in the two HCV serum samples tested, 65.3% → 55.6% → 33.7% (#4727) and 68.6% → 56% → 21% (#4970), respectively for no normalization, self- and driver-applied normalization. However, this approach was associated with a loss of HCV sequences perhaps due to its micro-homology with the human genome. In capture sequencing, HCV-mapped sequencing reads occupied 96.8% (#4727) and 22.14% (#4970) in NGS data, equivalent to 1936x and 7380x enrichment, respectively. Capture sequencing was then applied to ten serum samples collected at the end of HCV antiviral therapy. Interestingly, the number of HCV-mapped reads was significantly higher in relapsed patients ( n  = 5) than those from patients with sustained virological response (SVR) ( n  = 5), 102.4 ± 72.3 vs. 2.6 ± 0.55, p  = 0.014. Conclusions Our data provides concept evidence for a highly sensitive HCV detection by capture sequencing. The abundance difference of HCV sequencing reads at the end of HCV antiviral therapy could be applied to predict treatment outcomes.
    Type of Medium: Online Resource
    ISSN: 1471-2334
    URL: Article
    DOI: 10.1186/s12879-020-05355-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2041550-3
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  • 5
    Online Resource
    Online Resource
    Within-host quantitation of anellovirus genome complexity from clinical samples
    Elsevier BV ; 2022
    In:  Journal of Virological Methods Vol. 302 ( 2022-04), p. 114493-
    Details
    In: Journal of Virological Methods, Elsevier BV, Vol. 302 ( 2022-04), p. 114493-
    Type of Medium: Online Resource
    ISSN: 0166-0934
    URL: Article
    DOI: 10.1016/j.jviromet.2022.114493
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2007929-1
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion
    Springer Science and Business Media LLC ; 2020
    In:  BMC Research Notes Vol. 13, No. 1 ( 2020-12)
    Details
    In: BMC Research Notes, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2020-12)
    Abstract: Owing to the overwhelming dominance of human and commensal microbe sequences, low efficiency is a major concern in clinical viral sequencing using next-generation sequencing. DNA composed of 7-deaza-2′-deoxyguanosine 5′-triphosphate (c 7 dGTP), an analog of deoxyguanosine triphosphate (dGTP), is resistant to selective restriction enzymes. This characteristic has been utilized to develop a novel strategy for target enrichment in next-generation sequencing. Results The new enrichment strategy is named target enrichment via enzymatic digestion in next-generation sequencing (TEEDseq). It combined 7-deaza-2′-deoxyguanosine 5′-triphosphate (c 7 dGTP)-involved primer extension, splinter-assisted intracellular cyclization, c 7 dGTP)-resistant enzymatic digestion, and two-phase rolling cycle amplification. We first estimated c7dGTP for its efficiency in PCR amplification and its resistance to three restriction enzymes, AluI, HaeIII, and HpyCH4V. We then evaluated TEEDseq using a serum sample spiked with a 1311-bp hepatitis B virus (HBV) fragment. TEEDseq achieved an HBV on-target rate of 3.31 ± 0.39%, which was equivalent to 454× the enrichment of direct Illumina sequencing. Therefore, the current study has provided a concept proof for TEEDseq as an alternative option for clinical viral sequencing that requires an enrichment in next-generation sequencing.
    Type of Medium: Online Resource
    ISSN: 1756-0500
    URL: Article
    DOI: 10.1186/s13104-020-05292-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2413336-X
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  • 7
    Online Resource
    Online Resource
    Early goal-directed mobilization in patients with acute type A aortic dissection: A randomized controlled trial
    SAGE Publications ; 2023
    In:  Clinical Rehabilitation Vol. 37, No. 10 ( 2023-10), p. 1311-1321
    Details
    In: Clinical Rehabilitation, SAGE Publications, Vol. 37, No. 10 ( 2023-10), p. 1311-1321
    Abstract: To determine the safety and efficacy of early postoperative mobilization in patients who have undergone surgical repair of acute type A aortic dissection. Design Randomized controlled trial. Setting Heart Medical Center. Subjects Seventy-seven patients with acute type A aortic dissection were assessed. Intervention Patients were randomly allocated into: (1) the control group (usual care) ( n  =  38) and (2) the intervention group (early goal-directed mobilization) ( n  =  39). Main Measures The primary outcome was the patient's functional status. The secondary outcomes included vital signs, serious adverse events, muscle strength, intensive care unit-acquired weakness, grip strength, duration of mechanical ventilation, length of stay, readmission rate, and health-related quality of life after 3 months. Results The vital signs of the patients were within the tolerable ranges during the entire intervention. No serious exercise-related adverse events were observed in the intervention group. The Barthel Index score ( P  =  0.013), Medical Research Council score ( P  =  0.001), grip strength ( P  =  0.001), and health-related quality of life ( P  =  0.001) were higher in the intervention group. Intensive care unit acquired weakness ( P  =  0.019), duration of mechanical ventilation ( P  =  0.002), intensive care unit stay ( P  =  0.002), and total length of stay ( P  =  0.010) were lower in the intervention group. Patients in the intervention group had a higher physical health-related quality of life ( P  =  0.015) at 3 months post-surgery. There was no difference in readmission rates. Conclusions Delivery of early goal-directed mobilization in acute type A aortic dissection was safe and facilitated the recovery of daily living ability, shorter hospital stay, and improved quality of life after discharge.
    Type of Medium: Online Resource
    ISSN: 0269-2155 , 1477-0873
    URL: Article
    DOI: 10.1177/02692155231169822
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2028323-4
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  • 8
    Online Resource
    Online Resource
    A family intervention to prevent postoperative delirium in patients undergoing cardiac valve surgery: A randomized controlled study
    Elsevier BV ; 2024
    In:  Heart & Lung Vol. 63 ( 2024-01), p. 1-8
    Details
    In: Heart & Lung, Elsevier BV, Vol. 63 ( 2024-01), p. 1-8
    Type of Medium: Online Resource
    ISSN: 0147-9563
    URL: Article
    DOI: 10.1016/j.hrtlng.2023.09.002
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 2006428-7
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  • 9
    Online Resource
    Online Resource
    Corrosion-Resistant Zeolite Silicalite-1 Coatings Synthesized by Seeded Growth
    American Chemical Society (ACS) ; 2012
    In:  Industrial & Engineering Chemistry Research Vol. 51, No. 9 ( 2012-03-07), p. 3646-3652
    Details
    In: Industrial & Engineering Chemistry Research, American Chemical Society (ACS), Vol. 51, No. 9 ( 2012-03-07), p. 3646-3652
    Type of Medium: Online Resource
    ISSN: 0888-5885 , 1520-5045
    URL: Article
    DOI: 10.1021/ie202080a
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2012
    detail.hit.zdb_id: 1484436-9
    detail.hit.zdb_id: 56690-1
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  • 10
    Online Resource
    Online Resource
    Amino Acid Imprinted UiO-66s for Highly Recognized Adsorption of Small Angiotensin-Converting-Enzyme-Inhibitory Peptides
    American Chemical Society (ACS) ; 2019
    In:  ACS Applied Materials & Interfaces Vol. 11, No. 26 ( 2019-07-03), p. 23039-23049
    Details
    In: ACS Applied Materials & Interfaces, American Chemical Society (ACS), Vol. 11, No. 26 ( 2019-07-03), p. 23039-23049
    Type of Medium: Online Resource
    ISSN: 1944-8244 , 1944-8252
    URL: Article
    DOI: 10.1021/acsami.9b07453
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2019
    detail.hit.zdb_id: 2467494-1
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