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  • 1
    Online Resource
    Online Resource
    The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells
    MDPI AG ; 2019
    In:  International Journal of Molecular Sciences Vol. 20, No. 23 ( 2019-12-03), p. 6099-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 23 ( 2019-12-03), p. 6099-
    Abstract: Protein acetylation affects gene expression, as well as other processes in cells, and it might be dependent on the availability of the metals. However, whether iron chelating compounds (siderophores) can have an effect on the acetylation process in plant roots is largely unknown. In the present study, western blotting and confocal microscopy was used to examine the degree of acetylation of histone H3 and alpha tubulin in Pinus sylvestris root cells in the presence of structurally different siderophores. The effect of metabolites that were produced by pathogenic and mycorrhizal fungi was also assessed. No effect was observed on histone acetylation. By contrast, the metabolites of the pathogenic fungus were able to decrease the level of microtubule acetylation, whereas treatment with iron-free ferrioxamine (DFO) was able to increase it. This latter was not observed when ferrioxamine-iron complexes were used. The pathogen metabolites induced important modifications of cytoskeleton organization. Siderophores also induced changes in the tubulin skeleton and these changes were iron-dependent. The effect of siderophores on the microtubule network was dependent on the presence of iron. More root cells with a depolymerized cytoskeleton were observed when the roots were exposed to iron-free siderophores and the metabolites of pathogenic fungi; whereas, the metabolites from mycorrhizal fungi and iron-enriched forms of siderophores slightly altered the cytoskeleton network of root cells. Collectively, these data indicated that the metabolites of pathogenic fungi mirror siderophore action, and iron limitation can lead to enhanced alternations in cell structure and physiology.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms20236099
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Temperature Regulation of Primary and Secondary Seed Dormancy in Rosa canina L.: Findings from Proteomic Analysis
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 19 ( 2020-09-23), p. 7008-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 19 ( 2020-09-23), p. 7008-
    Abstract: Temperature is a key environmental factor restricting seed germination. Rose (Rosa canina L.) seeds are characterized by physical/physiological dormancy, which is broken during warm, followed by cold stratification. Exposing pretreated seeds to 20 °C resulted in the induction of secondary dormancy. The aim of this study was to identify and functionally characterize the proteins associated with dormancy control of rose seeds. Proteins from primary dormant, after warm and cold stratification (nondormant), and secondary dormant seeds were analyzed using 2-D electrophoresis. Proteins that varied in abundance were identified by mass spectrometry. Results showed that cold stratifications affected the variability of the highest number of spots, and there were more common spots with secondary dormancy than with warm stratification. The increase of mitochondrial proteins and actin during dormancy breaking suggests changes in cell functioning and seed preparation to germination. Secondary dormant seeds were characterized by low levels of legumin, metabolic enzymes, and actin, suggesting the consumption of storage materials, a decrease in metabolic activity, and cell elongation. Breaking the dormancy of rose seeds increased the abundance of cellular and metabolic proteins that promote germination. Induction of secondary dormancy caused a decrease in these proteins and germination arrest.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21197008
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Proteomic analysis of black poplar (Populus nigra L.) seed storability
    Springer Science and Business Media LLC ; 2019
    In:  Annals of Forest Science Vol. 76, No. 4 ( 2019-12)
    Details
    In: Annals of Forest Science, Springer Science and Business Media LLC, Vol. 76, No. 4 ( 2019-12)
    Abstract: Adequate storability of black poplar ( Populus nigra L.) seeds at temperatures below 0 °C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining long-term stability of dehydrated tissue. Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. Methods Dried seeds (7.1% MC) were stored at 3 °C, − 3 °C, − 20 °C, and − 196 °C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (p I ) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. Results A germination test showed that seeds remained viable at − 3, − 20, and − 196 °C. Storage at 3 °C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.
    Type of Medium: Online Resource
    ISSN: 1286-4560 , 1297-966X
    URL: Article
    DOI: 10.1007/s13595-019-0887-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2012340-1
    SSG: 23
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  • 4
    Online Resource
    Online Resource
    DNA synthesis pattern, proteome, and ABA and GA signalling in developing seeds of Norway maple (Acer platanoides)
    CSIRO Publishing ; 2019
    In:  Functional Plant Biology Vol. 46, No. 2 ( 2019), p. 152-
    Details
    In: Functional Plant Biology, CSIRO Publishing, Vol. 46, No. 2 ( 2019), p. 152-
    Abstract: Mature seeds of Norway maple exhibit desiccation tolerance and deep physiological dormancy. Flow cytometry, proteomics, and immunodetection have been combined to investigate seed development of this species. DNA content analysis revealed that cell cycle/endoreduplication activity differs between seed organs and developmental stages. In the embryo axis, the proportion of the nuclei with the highest DNA content (4C) increases at the beginning of maturation (17 weeks after flowering; WAF), and then is stable until the end of maturation, to increase again after drying. In cotyledons, during maturation endopolyploid nuclei (8C) occur and the intensity of endoreduplication increases up to 21 WAF, and then is stable until development is completed. In dry mature seeds, the proportion of 4C nuclei is high, and reaches 36% in the embryo axis and 52% in cotyledons. Proteomic studies revealed that energy and carbon metabolism, fatty acid biosynthesis, storage and antioxidant proteins are associated with seed development. Study of the ABI5 protein, a transcription factor involved in ABA signalling, and the RGL2 protein, a repressor of the GA signalling indicates that the highest accumulation of these proteins occurs in fully-matured and dried seeds. It is suggested that this increase in accumulation can be associated with completion of maturation, mainly with desiccation and dormancy acquisition.
    Type of Medium: Online Resource
    ISSN: 1445-4408
    URL: Article
    DOI: 10.1071/FP18074
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2019
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Regulation of Seed Dormancy and Germination Mechanisms in a Changing Environment
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 3 ( 2021-01-29), p. 1357-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 3 ( 2021-01-29), p. 1357-
    Abstract: Environmental conditions are the basis of plant reproduction and are the critical factors controlling seed dormancy and germination. Global climate change is currently affecting environmental conditions and changing the reproduction of plants from seeds. Disturbances in germination will cause disturbances in the diversity of plant communities. Models developed for climate change scenarios show that some species will face a significant decrease in suitable habitat area. Dormancy is an adaptive mechanism that affects the probability of survival of a species. The ability of seeds of many plant species to survive until dormancy recedes and meet the requirements for germination is an adaptive strategy that can act as a buffer against the negative effects of environmental heterogeneity. The influence of temperature and humidity on seed dormancy status underlines the need to understand how changing environmental conditions will affect seed germination patterns. Knowledge of these processes is important for understanding plant evolution and adaptation to changes in the habitat. The network of genes controlling seed dormancy under the influence of environmental conditions is not fully characterized. Integrating research techniques from different disciplines of biology could aid understanding of the mechanisms of the processes controlling seed germination. Transcriptomics, proteomics, epigenetics, and other fields provide researchers with new opportunities to understand the many processes of plant life. This paper focuses on presenting the adaptation mechanism of seed dormancy and germination to the various environments, with emphasis on their prospective roles in adaptation to the changing climate.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22031357
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
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  • 6
    Online Resource
    Online Resource
    Plant development reprogramming by cynipid gall wasp: proteomic analysis
    Springer Science and Business Media LLC ; 2017
    In:  Acta Physiologiae Plantarum Vol. 39, No. 5 ( 2017-5)
    Details
    In: Acta Physiologiae Plantarum, Springer Science and Business Media LLC, Vol. 39, No. 5 ( 2017-5)
    Type of Medium: Online Resource
    ISSN: 0137-5881 , 1861-1664
    URL: Article
    DOI: 10.1007/s11738-017-2414-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2245807-4
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Proteome analysis of Norway maple (Acer platanoides L.) seeds dormancy breaking and germination: influence of abscisic and gibberellic acids
    Springer Science and Business Media LLC ; 2009
    In:  BMC Plant Biology Vol. 9, No. 1 ( 2009), p. 48-
    Details
    In: BMC Plant Biology, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2009), p. 48-
    Type of Medium: Online Resource
    ISSN: 1471-2229
    URL: Article
    DOI: 10.1186/1471-2229-9-48
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2009
    detail.hit.zdb_id: 2059868-3
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  • 8
    Online Resource
    Online Resource
    Identification and Classification of bcl Genes and Proteins of Bacillus cereus Group Organisms and Their Application in Bacillus anthracis Detection and Fingerprinting
    American Society for Microbiology ; 2009
    In:  Applied and Environmental Microbiology Vol. 75, No. 22 ( 2009-11-15), p. 7163-7172
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 75, No. 22 ( 2009-11-15), p. 7163-7172
    Abstract: The Bacillus cereus group includes three closely related species, B. anthracis , B. cereus , and B. thuringiensis , which form a highly homogeneous subdivision of the genus Bacillus . One of these species, B. anthracis , has been identified as one of the most probable bacterial biowarfare agents. Here, we evaluate the sequence and length polymorphisms of the Bacillus collagen-like protein bcl genes as a basis for B. anthracis detection and fingerprinting. Five genes, designated bclA to bclE , are present in B. anthracis strains. Examination of bclABCDE sequences identified polymorphisms in bclB alleles of the B. cereus group organisms. These sequence polymorphisms allowed specific detection of B. anthracis strains by PCR using both genomic DNA and purified Bacillus spores in reactions. By exploiting the length variation of the bcl alleles it was demonstrated that the combined bclABCDE PCR products generate markedly different fingerprints for the B. anthracis Ames and Sterne strains. Moreover, we predict that bclABCDE length polymorphism creates unique signatures for B. anthracis strains, which facilitates identification of strains with specificity and confidence. Thus, we present a new diagnostic concept for B. anthracis detection and fingerprinting, which can be used alone or in combination with previously established typing platforms.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.01069-09
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Signalling regulators of abscisic and gibberellic acid pathways are involved in dormancy breaking of Norway maple (Acer platanoides L.) seeds
    Springer Science and Business Media LLC ; 2017
    In:  Acta Physiologiae Plantarum Vol. 39, No. 11 ( 2017-11)
    Details
    In: Acta Physiologiae Plantarum, Springer Science and Business Media LLC, Vol. 39, No. 11 ( 2017-11)
    Type of Medium: Online Resource
    ISSN: 0137-5881 , 1861-1664
    URL: Article
    DOI: 10.1007/s11738-017-2544-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2245807-4
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Molecular and structural changes in vegetative buds of Norway spruce during dormancy in natural weather conditions
    Oxford University Press (OUP) ; 2018
    In:  Tree Physiology Vol. 38, No. 5 ( 2018-05-01), p. 721-734
    Details
    In: Tree Physiology, Oxford University Press (OUP), Vol. 38, No. 5 ( 2018-05-01), p. 721-734
    Type of Medium: Online Resource
    ISSN: 1758-4469
    URL: Article
    DOI: 10.1093/treephys/tpx156
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2018
    detail.hit.zdb_id: 1473475-8
    SSG: 12
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