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  • 1
    In: Science and Innovation, National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka) (Publications), Vol. 18, No. 1 ( 2022-02-14), p. 44-55
    Abstract: Вступ. Глобальні кліматичні зміни спричинюють нерівномірність опадів, що погіршує водно-фізичні властивості ґрунту та порушує мікробіологічні процеси. Як наслідок може виникати алелопатична ґрунтовтома.Проблематика. Пошук й розроблення нових ефективних та безпечних заходів регулювання фізіолого-біохімічного, алелопатичного й мікробіологічного стану системи рослина–ґрунт є нагальною необхідністю за умов нестабільноїекологічної ситуації.Мета. Оптимізувати фізіолого-біохімічні, алелопатичні й мікробіологічні характеристики системи рослина–ґрунт за умов недостатнього вологозабезпечення та ґрунтовтоми шляхом застосування кремнійвмісного мінералу анальциму.Матеріали й методи. У модельних вегетаційних дослідах вивчали вплив кремнійвмісного мінералу анальцимуу концентрації 0, 100, 200 та 300 мг на 200 мл ґрунтового субстрату на показники росту, водного режиму рослин пшениці та кукурудзи за різних умов зволоження (20, 40 та 60% повної вологоємкості) та типу ґрунтового субстрату. У польовому експерименті анальцим вносили в ґрунтовий субстрат перед посадкою насіння під рослини цукрового буряку у нормі 50 кг/га. У модельних та польових дослідах аналізували перебіг редокс-процесів, вміст фенолів та мікробіологічні показники ґрунту.Результати. Використання анальциму оптимізувало показники росту, водного режиму рослин, а також алелопатичні характеристики системи рослина–ґрунт шляхом зниження вмісту вільних фенолів, активізації розвитку мікробних ценозів й редокс-процесів. При цьому відмічено відсутність фітотоксичного прояву анальциму,Висновки. Запропоновано застосування анальциму для покращення фізіолого-біохімічних, алелопатичних й мікробіологічних властивостей системи рослина–ґрунт. Окреслено перспективи використання мінералу для підвищення адаптаційної здатності рослин за умов посухи та для подолання наслідків ґрунтовтоми.
    Type of Medium: Online Resource
    ISSN: 2413-4996 , 2409-9066
    URL: Issue
    Language: Unknown
    Publisher: National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka) (Publications)
    Publication Date: 2022
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  • 2
    Online Resource
    Online Resource
    M.M. Gryshko National Botanical Garden of the NAS of Ukraine ; 2020
    In:  Plant Introduction Vol. 87-88 ( 2020-12-30), p. 3-21
    In: Plant Introduction, M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Vol. 87-88 ( 2020-12-30), p. 3-21
    Abstract: The study aimed to develop a modern, cheap, and environmentally safe technology for the disposal of plant waste, with the participation of the most active microorganisms-destructors.The microorganisms’ ability to help transform plant waste into viable, fertile soil was extensively studied. We selected strains of micromycetes Penicillium roseopurpureum, Trichoderma hamatum, T. koningii, Alternaria alternata, and bacteria of the genus Cytophaga, which are characterized by high growth rate and the absence of phytotoxicity. To accelerate microorganism development, we used silicon-containing mineral analcime, which contained immobilized spores of micromycetes and bacterial cells’ suspensions. Modified analcime was added to the waste in a ratio of 10 : 1. The plant remains prepared in this method were analyzed under conditions of both model and vegetation experiments.An evidence for the expediency of using the silicon-containing mineral analcime as a starting substrate for immobilization of spores and suspension of bacterial cells in the culture fluid was provided. The microorganisms involved in the experiment showed a positive result in transforming plant waste during the 30-day observation period. The highest destructive activity against apple and grape waste is characteristic for the T. hamatum strain, for beet waste – P. roseopurpureum. The species-specificity of these destructive microorganisms on plant growth processes was proved. The maximum growth of corn sprouts in apple waste was detected by inoculation with T. koningii spores, grape waste – T. hamatum, and beet waste – a mixture of micromycetes with a Cytophaga sp. suspension. The optimal duration of plant waste transformation using analcime, inoculated with microorganisms, is 20–30 days. In the indoor farming conditions, the standard for utilizing the modified vegetable waste placement was 10 % of the total volume of a substrate during the preparation of soil mixes.The environmental safety of plant waste after their destruction was confirmed. The presence of a silicon-containing mineral in the mixture leads to increased growth and plant development, optimization of the agrophysical, agrochemical, and biological parameters of the soil, reducing soil fatigue, and increasing fertility.
    Type of Medium: Online Resource
    ISSN: 2663-290X , 1605-6574
    URL: Issue
    Language: Unknown
    Publisher: M.M. Gryshko National Botanical Garden of the NAS of Ukraine
    Publication Date: 2020
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Macrothink Institute, Inc. ; 2018
    In:  Journal of Applied Biotechnology Vol. 6, No. 2 ( 2018-05-03), p. 11-
    In: Journal of Applied Biotechnology, Macrothink Institute, Inc., Vol. 6, No. 2 ( 2018-05-03), p. 11-
    Abstract: Goat anti-rabbit IgGs were purified from serum using Protein G affinity chromatography. The purity of eluted fractions was tested using SDS-PAGE, and the functionality of IgGs was tested in Western Blot analysis of Erythropoietin, after conjugation with Horseradish Peroxidase.
    Type of Medium: Online Resource
    ISSN: 2327-0640
    URL: Issue
    Language: Unknown
    Publisher: Macrothink Institute, Inc.
    Publication Date: 2018
    detail.hit.zdb_id: 2777457-0
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  • 4
    Online Resource
    Online Resource
    The American Association of Immunologists ; 2021
    In:  The Journal of Immunology Vol. 206, No. 1_Supplement ( 2021-05-01), p. 97.03-97.03
    In: The Journal of Immunology, The American Association of Immunologists, Vol. 206, No. 1_Supplement ( 2021-05-01), p. 97.03-97.03
    Abstract: PSTPIP2 is an adaptor protein, which binds multiple regulators of neutrophil signaling to suppress inflammation. The compete loss of PSTPIP2 protein in the mouse strain Pstpip2cmo results in the development of autoinflammatory disease chronic multifocal osteomyelitis (CMO), a condition resembling human CRMO. The disease is driven by an overproduction of IL-1β and deregulated activity of NADPH oxidase. The main goal of this study is to analyze the effects of PSTPIP2 interactions on leukocyte signaling pathways, IL-1β processing, ROS production by NADPH oxidase, and auto inflammation in vivo. To achieve this, we have generated mice with mutations in major interaction sites in PSTPIP2 including W232 responsible for binding to PEST family phosphatases (PEST-PTP), and the C-terminal sequence containing major tyrosine phosphorylation sites known to bind inositide phosphatase SHIP1 (Pstpip2ΔC-t). W232A mutation resulted in enhanced ROS production by neutrophils and increased IL-1β cleavage. Moreover, some W232A mutants developed CMO symptoms. However, the kinetics was delayed and severity was milder, when compared to Pstpip2cmo strain. In contrast, Pstpip2ΔC-t mutants did not show any CMO symptoms or increased IL-1β cleavage. Interestingly, partially deregulated ROS production could still be measured in Pstpip2ΔC-t neutrophils. Collectively, our data demonstrate an important function of PEST-PTP binding to PSTPIP2 in the suppression of auto inflammation, while SHIP1 binding contributes to the regulation of neutrophil oxidative burst. The obtained data give us a novel perspective on PSTPIP2 interactome and extend our knowledge of the molecular interactions regulating physiological and pathological inflammation.
    Type of Medium: Online Resource
    ISSN: 0022-1767 , 1550-6606
    RVK:
    RVK:
    Language: English
    Publisher: The American Association of Immunologists
    Publication Date: 2021
    detail.hit.zdb_id: 1475085-5
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  • 5
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 24, No. 2 ( 2020-01), p. 1980-1992
    Abstract: WW domain binding protein 1‐like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6‐RUNX1 (t(12;21)(p13;q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non‐haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4‐family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l ‐deficient mice revealed alterations in B cell development and enhanced efficiency of bone marrow cell transplantation. Collectively, our data show that WBP1L is a novel regulator of CXCR4 signalling and haematopoiesis.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2076114-4
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  • 6
    In: Journal of Biological Chemistry, Elsevier BV, Vol. 297, No. 4 ( 2021-10), p. 101131-
    Type of Medium: Online Resource
    ISSN: 0021-9258
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2141744-1
    detail.hit.zdb_id: 1474604-9
    SSG: 12
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  • 7
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 13 ( 2022-12-20)
    Abstract: Autoinflammatory diseases are characterized by dysregulation of innate immune system leading to spontaneous sterile inflammation. One of the well-established animal models of this group of disorders is the mouse strain Pstpip2 cmo . In this strain, the loss of adaptor protein PSTPIP2 leads to the autoinflammatory disease chronic multifocal osteomyelitis. It is manifested by sterile inflammation of the bones and surrounding soft tissues of the hind limbs and tail. The disease development is propelled by elevated production of IL-1β and reactive oxygen species by neutrophil granulocytes. However, the molecular mechanisms linking PSTPIP2 and these pathways have not been established. Candidate proteins potentially involved in these mechanisms include PSTPIP2 binding partners, PEST family phosphatases (PEST-PTPs) and phosphoinositide phosphatase SHIP1. Methods To address the role of these proteins in PSTPIP2-mediated control of inflammation, we have generated mouse strains in which PEST-PTP or SHIP1 binding sites in PSTPIP2 have been disrupted. In these mouse strains, we followed disease symptoms and various inflammation markers. Results Our data show that mutation of the PEST-PTP binding site causes symptomatic disease, whereas mice lacking the SHIP1 interaction site remain asymptomatic. Importantly, both binding partners of PSTPIP2 contribute equally to the control of IL-1β production, while PEST-PTPs have a dominant role in the regulation of reactive oxygen species. In addition, the interaction of PEST-PTPs with PSTPIP2 regulates the production of the chemokine CXCL2 by neutrophils. Its secretion likely creates a positive feedback loop that drives neutrophil recruitment to the affected tissues. Conclusions We demonstrate that PSTPIP2-bound PEST-PTPs and SHIP1 together control the IL-1β pathway. In addition, PEST-PTPs have unique roles in the control of reactive oxygen species and chemokine production, which in the absence of PEST-PTP binding to PSTPIP2 shift the balance towards symptomatic disease.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2606827-8
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  • 8
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  HemaSphere Vol. 7, No. S3 ( 2023-08), p. e28037c1-
    In: HemaSphere, Ovid Technologies (Wolters Kluwer Health), Vol. 7, No. S3 ( 2023-08), p. e28037c1-
    Type of Medium: Online Resource
    ISSN: 2572-9241
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2023
    detail.hit.zdb_id: 2922183-3
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  • 9
    Online Resource
    Online Resource
    Scientific Research Publishing, Inc. ; 2019
    In:  American Journal of Molecular Biology Vol. 09, No. 02 ( 2019), p. 52-63
    In: American Journal of Molecular Biology, Scientific Research Publishing, Inc., Vol. 09, No. 02 ( 2019), p. 52-63
    Type of Medium: Online Resource
    ISSN: 2161-6620 , 2161-6663
    Language: Unknown
    Publisher: Scientific Research Publishing, Inc.
    Publication Date: 2019
    detail.hit.zdb_id: 2619421-1
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  • 10
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 12 ( 2021-4-27)
    Abstract: LST1 is a small adaptor protein expressed in leukocytes of myeloid lineage. Due to the binding to protein tyrosine phosphatases SHP1 and SHP2 it was thought to have negative regulatory function in leukocyte signaling. It was also shown to be involved in cytoskeleton regulation and generation of tunneling nanotubes. LST1 gene is located in MHCIII locus close to many immunologically relevant genes. In addition, its expression increases under inflammatory conditions such as viral infection, rheumatoid arthritis and inflammatory bowel disease and its deficiency was shown to result in slightly increased sensitivity to influenza infection in mice. However, little else is known about its role in the immune system homeostasis and immune response. Here we show that similar to humans, LST1 is expressed in mice in the cells of the myeloid lineage. In vivo , its deficiency results in alterations in multiple leukocyte subset abundance in steady state and under inflammatory conditions. Moreover, LST1-deficient mice show significant level of resistance to dextran sodium sulphate (DSS) induced acute colitis, a model of inflammatory bowel disease. These data demonstrate that LST1 regulates leukocyte abundance in lymphoid organs and inflammatory response in the gut.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2606827-8
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