In:
Molecular Reproduction and Development, Wiley, Vol. 80, No. 12 ( 2013-12), p. 1035-1047
Abstract:
Human adipose tissue‐derived mesenchymal stem cells (hAT‐MSCs) secrete bioactive materials that are beneficial for tissue repair and regeneration. In this study, we characterized human hAT‐MSC bioactive material (hAT‐MSC‐BM), and examined the effect of hAT‐MSC‐BM on porcine embryo development. hAT‐MSC‐BM was enriched with several growth factors and cytokines, including fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA), and interleukin 6 (IL6). Among the various concentrations and days of treatment tested, 10% hAT‐MSC‐BM treatment beginning on culture Day 4 provided the best environment for the in vitro growth of parthenogenetic porcine embryos. While the addition of 10% fetal bovine serum (FBS) increased the hatching rate and the total cell number of parthenogenetic porcine embryos compared with the control and hAT‐MSC culture medium group, the best results were from the group cultured with 10% hAT‐MSC‐BM. Mitochondrial activity was also higher in the 10% hAT‐MSC‐BM‐treated group. Moreover, the relative mRNA expression levels of development and anti‐apoptosis genes were significantly higher in the 10% hAT‐MSC‐BM‐treated group than in control, hAT‐MSC culture medium, or 10% FBS groups, whereas the transcript abundance of an apoptosis gene was slightly lower. Treatment with 10% hAT‐MSC‐BM starting on Day 4 also improved the development rate and the total cell number of in vitro‐fertilized embryos. This is the first report on the benefits of hAT‐MSC‐BM in a porcine embryo in vitro culture system. We conclude that hAT‐MSC‐BM is a new, alternative supplement that can improve the development of porcine embryos during both parthenogenesis and fertilization in vitro. Mol. Reprod. Dev. 80: 1035–1047, 2013. © 2013 Wiley Periodicals, Inc .
Type of Medium:
Online Resource
ISSN:
1040-452X
,
1098-2795
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
1493888-1
SSG:
12
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