In:
The Plant Pathology Journal, Korean Society of Plant Pathology, Vol. 39, No. 1 ( 2023-02-01), p. 52-61
Abstract:
Fire blight, caused by 〈 i 〉 Erwinia amylovora 〈 /i 〉 , is one of the major bacterial disease of apple and pear, causing enormous economic losses worldwide. Several control measures against 〈 i 〉 E. amylovora 〈 /i 〉 have been reported till date, however, none of them have proved to be effective significantly against the pathogen. In this study, mechanisms of the copper-based control agents (CBCAs): copper oxychloride (COCHL), copper oxide (COX), copper hydroxide (CHY), copper sulfate basic (CSB), and tribasic copper sulfate (TCS) and their disease severity reduction efficacy against 〈 i 〉 E. amylovora 〈 /i 〉 were analyzed. Bis-1,3-dibutylbarbituric acid trimethine oxonol, carboxyl fluorescein diacetate succinimidyl ester, and 5-cyano-2,3-ditolyl tetrazolium chloride staining were used to check the damage of membrane potential, cytoplasmic pH 〈 sub 〉 in 〈 /sub 〉 , and respiration of CBCAs-treated 〈 i 〉 E. amylovora 〈 /i 〉 , respectively. High disturbance in the membrane potential of 〈 i 〉 E. amylovora 〈 /i 〉 was found under COX and COCHL treatments. Similarly, higher significant changes in the inner cytoplasmic pH 〈 sub 〉 in 〈 /sub 〉 were observed under COX, COCHL, and TCS treatment. CHY and COCHL-treated 〈 i 〉 E. amylovora 〈 /i 〉 showed a significant reduction in respiration. 〈 i 〉 In vitro 〈 /i 〉 bioassay results revealed that CHY, CSB, and TCS at 2,000 ppm reduced the severity of fire blight both in pre- and post-treatment of CBCAs in immature apple fruits and seedlings. Overall, the most effective CBCAs against 〈 i 〉 E. amylovora 〈 /i 〉 could be CHY at 2,000 ppm as its showed inhibition mechanisms and disease severity reduction.
Type of Medium:
Online Resource
ISSN:
1598-2254
,
2093-9280
DOI:
10.5423/PPJ.OA.07.2022.0100
Language:
English
Publisher:
Korean Society of Plant Pathology
Publication Date:
2023
detail.hit.zdb_id:
2529195-6
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