In:
The Journal of Immunology, The American Association of Immunologists, Vol. 151, No. 1 ( 1993-07-01), p. 92-99
Abstract:
The early stages of lymphoid differentiation preceding T and B lineage commitment remain poorly defined. We hypothesized that early lymphoid precursor cells are possibly common progenitors and would express a very early T cell-associated Ag (CD7) and a very early B cell-associated Ag (CD19) simultaneously. We therefore transformed CD7+CD19+ fetal bone marrow lymphoid cells using EBV. Extensive characterization of the resulting cell lines indicated that two cell lines corresponded to pre-B and early B cells co-expressing CD7. The third cell line resembled a thymocyte, which co-expressed a number of B cell-associated Ag including CD19 and the stem cell Ag CD34. The two predominantly B lineage cell lines have their Ig genes rearranged, whereas the predominantly T lineage cell line has TCR and Ig H chain genes rearranged. Cross-lineage Ag were not expressed any more after culturing for a prolonged period of time, i.e., B lineage cells became CD7 negative and the thymocyte lineage became negative for the B cell-associated Ag. However, in all three cell lines TCR and/or Ig gene rearrangements remained unchanged. These observations support the existence of a common lymphoid precursor co-expressing CD7 and CD19 that gives rise to either T or B cells.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.151.1.92
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1993
detail.hit.zdb_id:
1475085-5
detail.hit.zdb_id:
3056-9
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