In:
European Journal of Biochemistry, Wiley, Vol. 170, No. 1-2 ( 1987-12), p. 99-104
Abstract:
In order to study the tissue‐specific expression of rabbit apolipoprotein (apo) A‐I, a 923‐base‐pair clone, pRBA‐502, complementary to rabbit apo A‐I mRNA was identified from a rabbit intestinal cDNA library by hybrid‐select translation and immunoprecipitation methods. Northern blot and dot‐blot hybridization, utilizing 32 P‐labeled pRBA‐502, revealed that the rabbit apo A‐I gene is expressed in the intestine, not in the liver and that rabbit apo A‐I mRNA is about 950 nucleotides in length. The entire nucleotide sequence of pRBA‐502 has been determined and the complete amino acid sequence of the corresponding apo A‐I has been deduced. The mRNA codes for a protein comprising 265 amino acids. Amino acids 1–18 and 19–24 of the primary translation product represent the presegment and prosegment, respectively, of apo A‐I. Matured rabbit apo A‐I contains 241 amino acids and has a molecular mass of 27612 Da. Using pRBA‐502 as a probe, a 15.5‐kb genomic fragment, which contains the entire apo A‐I gene, was isolated from a rabbit liver genomic library. Sequence analysis of the gene shows that the 200 base pairs of the 5′ upstream flanking region of the rabbit and human apo A‐I genes showed 78% sequence homology. Like the human apo A‐I gene, the rabbit apo A‐I gene is interrupted by three intervening sequences. Except for two nucleotides in the fourth exon, the coding sequence of the rabbit liver apo A‐I gene is identical to that of pRBA‐502. Our data showed that the lack of expression of apo A‐I gene in rabbit liver is not due to the alternation of rabbit liver apo A‐I gene sequence and suggest that the expression of apo A‐I gene in rabbit liver is regulated by a trans ‐acting regulating element(s).
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1987.170.issue-1-2
DOI:
10.1111/j.1432-1033.1987.tb13672.x
Language:
English
Publisher:
Wiley
Publication Date:
1987
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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