In:
International Journal of Rheumatic Diseases, Wiley, Vol. 21, No. 8 ( 2018-08), p. 1619-1626
Abstract:
Previous association studies have identified genetic variants in the human leukocyte antigen ( HLA ) complex as substantial risk factors for idiopathic inflammatory myopathies ( IIM s). However, a great number of genes are located in the HLA region, and thus fine mapping is quite necessary. Methods Targeted capture sequencing were performed on the whole HLA region in 42 IIM patients and 24 healthy controls. A microarray analysis was applied to analyze gene expression profiles in additional 20 newly diagnosed IIM and five healthy controls. Results The HLA region was confirmed to be associated with IIM s in Chinese patients. By gene expression profiling and pathway analysis, several genes were identified as candidates for IIM risk factors, including HLA ‐A , HLA ‐B , HLA ‐ DRB 5 , HLA ‐ DRB 1 , HLA ‐ DQA 1 , HLA ‐ DQB 1 and HLA ‐ DQB 2 . Interestingly, p.Y107V of the HLA ‐ DRB 1 was predicted to be a potential causal non‐synonymous variation for IIM s that may affect the antigen‐binding groove of the HLA ‐ II molecule. Conclusions Our data have revealed novel genetic variations in the HLA region of IIM patients and provide new insight into the pathogenesis and diagnosis of IIM s.
Type of Medium:
Online Resource
ISSN:
1756-1841
,
1756-185X
DOI:
10.1111/apl.2018.21.issue-8
DOI:
10.1111/1756-185X.13350
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2427877-4
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