In:
Journal of Virology, American Society for Microbiology, Vol. 95, No. 11 ( 2021-05-10)
Abstract:
The latent reservoir of HIV-1 is a major barrier for viral eradication. Potent HIV-1 broadly neutralizing antibodies (bNabs) have been used to prevent and treat HIV-1 infections in animal models and clinical trials. Combination of bNabs and latency-reversing agents (LRAs) is considered a promising approach for HIV-1 eradication. PCR-based assays that can rapidly and specifically measure singly spliced HIV-1 vpu / env mRNA are needed to evaluate the induction of the viral envelope production at the transcription level and bNab-mediated reservoir clearance. Here, we reported a PCR-based method to accurately quantify the production of intracellular HIV-1 vpu / env mRNA. With the vpu / env assay, we determined the LRA combinations that could effectively induce vpu / env mRNA production in CD4 + T cells from antiretroviral therapy (ART)-treated individuals. None of the tested LRAs were effective alone. A comparison between the quantitative viral outgrowth assay (Q-VOA) and the vpu / env assay showed that vpu / env mRNA production was closely associated with the reactivation of replication-competent HIV-1, suggesting that vpu / env mRNA was mainly produced by intact viruses. Finally, antibody-mediated killing in HIV-1-infected humanized mice demonstrated that the vpu / env assay could be used to measure the reduction of infected cells in tissues and was more accurate than the commonly used gag -based PCR assay, which measures unspliced viral genomic RNA. In conclusion, the vpu / env assay allows convenient and accurate assessment of HIV-1 latency reversal and bNab-mediated therapeutic strategies. IMPORTANCE HIV-1 persists in individuals on antiretroviral therapy (ART) due to the long-lived cellular reservoirs that contain dormant viruses. Recent discoveries of HIV-1-specific broadly neutralizing antibodies (bNabs) targeting HIV-1 Env protein rekindled the interest in antibody-mediated elimination of latent HIV-1. Latency-reversing agents (LRAs) together with HIV-1 bNabs is a possible strategy to clear residual viral reservoirs, which makes the evaluation of HIV-1 Env expression upon LRA treatment critical. We developed a PCR-based assay to quantify the production of intracellular HIV-1 vpu / env mRNA. Using patient CD4 + T cells, we found that induction of HIV-1 vpu / env mRNA required a combination of different LRAs. Using in vitro , ex vivo , and humanized mouse models, we showed that the vpu / env assay could be used to measure antibody efficacy in clearing HIV-1 infection. These results suggest that the vpu / env assay can accurately evaluate HIV-1 reactivation and bNab-based therapeutic interventions.
Type of Medium:
Online Resource
ISSN:
0022-538X
,
1098-5514
DOI:
10.1128/JVI.02124-20
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2021
detail.hit.zdb_id:
1495529-5
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