In:
Frontiers in Genetics, Frontiers Media SA, Vol. 12 ( 2021-6-30)
Abstract:
While the chicken ( Gallus gallus ) is the most consumed agricultural animal worldwide, the chicken transcriptome remains understudied. We have characterized the transcriptome of 10 cell and tissue types from the chicken using RNA-seq, spanning intestinal tissues (ileum, jejunum, proximal cecum), immune cells (B cells, bursa, macrophages, monocytes, spleen T cells, thymus), and reproductive tissue (ovary). We detected 17,872 genes and 24,812 transcripts across all cell and tissue types, representing 73% and 63% of the current gene annotation, respectively. Further quantification of RNA transcript biotypes revealed protein-coding and lncRNAs specific to an individual cell/tissue type. Each cell/tissue type also has an average of around 1.2 isoforms per gene, however, they all have at least one gene with at least 11 isoforms. Differential expression analysis revealed a large number of differentially expressed genes between tissues of the same category (immune and intestinal). Many of these differentially expressed genes in immune cells were involved in cellular processes relating to differentiation and cell metabolism as well as basic functions of immune cells such as cell adhesion and signal transduction. The differential expressed genes of the different segments of the chicken intestine (jejunum, ileum, proximal cecum) correlated to the metabolic processes in nutrient digestion and absorption. These data should provide a valuable resource in understanding the chicken genome.
Type of Medium:
Online Resource
ISSN:
1664-8021
DOI:
10.3389/fgene.2021.664424
DOI:
10.3389/fgene.2021.664424.s001
DOI:
10.3389/fgene.2021.664424.s002
DOI:
10.3389/fgene.2021.664424.s003
DOI:
10.3389/fgene.2021.664424.s004
DOI:
10.3389/fgene.2021.664424.s005
DOI:
10.3389/fgene.2021.664424.s006
DOI:
10.3389/fgene.2021.664424.s007
DOI:
10.3389/fgene.2021.664424.s008
DOI:
10.3389/fgene.2021.664424.s009
DOI:
10.3389/fgene.2021.664424.s010
DOI:
10.3389/fgene.2021.664424.s011
DOI:
10.3389/fgene.2021.664424.s012
DOI:
10.3389/fgene.2021.664424.s013
DOI:
10.3389/fgene.2021.664424.s014
DOI:
10.3389/fgene.2021.664424.s015
DOI:
10.3389/fgene.2021.664424.s016
DOI:
10.3389/fgene.2021.664424.s017
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606823-0
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