In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 43, No. 3 ( 2005-03), p. 1072-1075
Abstract:
Escherichia coli strain TUM2139 was isolated from a stool sample from a 9-year-old girl on 16 June 2004. This strain was categorized as Shiga toxin-producing Escherichia coli (STEC) because the Shiga-like toxin gene stx 1 was detected by immunochromatography and PCR assay. The strain was highly resistant to cefotaxime (256 μg/ml) and was also resistant to cefepime, cefpodoxime, ceftriaxone, and aztreonam. In the presence of 4 μg of clavulanic acid per ml, the MIC of cefotaxime decreased to ≤0.12 μg/ml, indicating that this strain was an extended-spectrum β-lactamase (ESBL) producer. Cefotaxime resistance was transferred to E. coli C600 by conjugation at a frequency of 3.0 × 10 −6 . A PCR assay was performed with primer sets specific for TEM-type and SHV-type ESBLs and for the CTX-M-2 (Toho-1), CTX-M-3, and CTX-M-9 groups of ESBLs. A specific signal was observed with the primer set specific for the CTX-M-9 group of β-lactamases. This β-lactamase was confirmed to be the ESBL CTX-M-18 by DNA sequencing. This is the first report of an ESBL-producing STEC isolate.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.43.3.1072-1075.2005
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2005
detail.hit.zdb_id:
1498353-9
SSG:
12
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