In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2022-8-5), p. e1010703-
Abstract:
Influenza A virus (IAV) infection during pregnancy initiates significant aortic endothelial and vascular smooth muscle dysfunction, with inflammation and T cell activation, but the details of the mechanism are yet to be clearly defined. Here we demonstrate that IAV disseminates preferentially into the perivascular adipose tissue (PVAT) of the aorta in mice. IAV mRNA levels in the PVAT increased at 1–3 days post infection (d.p.i) with the levels being ~4–8 fold higher compared with the vessel wall. IAV infection also increased Ly6C low patrolling monocytes and Ly6C high pro-inflammatory monocytes in the vessel wall at 3 d.p.i., which was then followed by a greater homing of these monocytes into the PVAT at 6 d.p.i. The vascular immune phenotype was characteristic of a “vascular storm”- like response, with increases in neutrophils, pro-inflammatory cytokines and oxidative stress markers in the PVAT and arterial wall, which was associated with an impairment in endothelium-dependent relaxation to acetylcholine. IAV also triggered a PVAT compartmentalised elevation in CD4 + and CD8 + activated T cells. In conclusion, the PVAT of the aorta is a niche that supports IAV dissemination and a site for perpetuating a profound innate inflammatory and adaptive T cell response. The manifestation of this inflammatory response in the PVAT following IAV infection may be central to the genesis of cardiovascular complications arising during pregnancy.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010703
DOI:
10.1371/journal.ppat.1010703.g001
DOI:
10.1371/journal.ppat.1010703.g002
DOI:
10.1371/journal.ppat.1010703.g003
DOI:
10.1371/journal.ppat.1010703.g004
DOI:
10.1371/journal.ppat.1010703.g005
DOI:
10.1371/journal.ppat.1010703.g006
DOI:
10.1371/journal.ppat.1010703.s001
DOI:
10.1371/journal.ppat.1010703.s002
DOI:
10.1371/journal.ppat.1010703.s003
DOI:
10.1371/journal.ppat.1010703.s004
DOI:
10.1371/journal.ppat.1010703.s005
DOI:
10.1371/journal.ppat.1010703.s006
DOI:
10.1371/journal.ppat.1010703.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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