In:
Reproduction, Fertility and Development, CSIRO Publishing, Vol. 9, No. 8 ( 1997), p. 781-
Abstract:
Early antral follicles 0·5–0·7 mm in diameter were
dissected from bovine ovaries, and oocyte–cumulus complexes with pieces of parietal granulosa (OCCGs) were then collected from the follicles. The
OCCGs containing oocytes of 90–99 m diameter (94·7 ± 2·8 µm, n = 196) were selected and
embedded in collagen gels and cultured for 14 days in TCM199 containing 10% fetal calf serum and 4 mM hypoxanthine. From cultured OCCGs, 144
surviving oocytes were recovered, of which 53 were granulosa cell-enclosed oocytes and 91 were denuded oocytes. The mean diameter of the surviving
oocytes was 114·2 ± 8·4 µm, significantly larger than that measured before culture (P 〈 0·05).
The granulosa cell-enclosed oocytes and denuded oocytes were further cultured for maturation for 24 h. After culture, 72% (38/53) of the
granulosa cell-enclosed oocytes and 59% (54/91) of the denuded oocytes showed normal morphology. These oocytes were then inseminated with
bovine spermatozoa. After 29 h of insemination, all of the denuded oocytes had degenerated, while 32% (12/38) of the granulosa cell-enclosed
oocytes showed normal morphology. Of the 12 oocytes, 5 were penetrated by spermatozoa, and 2 formed both male and female pronuclei. These results
demonstrate for the first time that bovine oocytes grown in vitro acquire meiotic competence and can be
penetrated by spermatozoa.
Type of Medium:
Online Resource
ISSN:
1031-3613
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
1997
SSG:
12
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