In:
Artificial Organs, Wiley, Vol. 40, No. 10 ( 2016-10), p. 938-949
Abstract:
Several materials are commercially available as substitutes for skin. However, new strategies are needed to improve the treatment of skin wounds. In this study, we developed and characterized a new device consisting of poly(lactic‐co‐glycolic acid) ( PLGA ) and collagen associated with mesenchymal stem cells derived from human adipose tissue. To develop the bilaminar device, we initially obtained a membrane of PLGA by dissolving the copolymer in chloroform and then produced a collagen type I scaffold by freeze‐drying. The materials were characterized physically by gel permeation chromatography, scanning electron microscopy, and mass loss. Biological activity was assessed by cell proliferation assay. A preliminary study in vivo was performed with a pig model in which tissue regeneration was assessed macroscopically and histologically, the commercial device Integra being used as a control. The PLGA /collagen bilaminar material was porous, hydrolytically degradable, and compatible with skin growth. The polymer complex allowed cell adhesion and proliferation, making it a potentially useful cell carrier. In addition, the transparency of the material allowed monitoring of the lesion when the dressings were changed. Xenogeneic mesenchymal cells cultured on the device ( PLGA /collagen/ ASC ) showed a reduced granulomatous reaction to bovine collagen, down‐regulation of α‐ SMA , enhancement in the number of neoformed blood vessels, and collagen organization as compared with normal skin; the device was superior to other materials tested ( PLGA /collagen and Integra) in its ability to stimulate the formation of new cutaneous tissue.
Type of Medium:
Online Resource
ISSN:
0160-564X
,
1525-1594
DOI:
10.1111/aor.2016.40.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2003825-2
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