In:
Genes to Cells, Wiley, Vol. 21, No. 4 ( 2016-04), p. 378-386
Abstract:
Drosophila produce a constant number of mechanosensory bristles called macrochaetae ( MC ), which develop from sensory organ precursor ( SOP ) cells within a proneural cluster ( PNC ). However, what ensures the precise determination of SOP cells remains to be elucidated. In this study, we conducted RNA i screening in PNC for genes involved in epigenetic regulation. We identified a H3K9 histone methyltransferase, SETDB 1/eggless, as a regulator of SOP development. Knockdown of SETDB 1 in PNC led to additional SOP s. We further tested the relationship between SETDB 1 and non‐apoptotic function of caspase on SOP development. Reinforcing caspase activation by heterozygous Drosophila inhibitor of apoptosis protein 1 ( DIAP 1) mutation rescued ectopic SOP development caused by SETDB 1 knockdown. Knockdown of SETDB 1 , however, had little effect on caspase activity. Simultaneous loss of SETDB 1 and caspase activity resulted in further increase in MC , indicating that the two components work cooperatively. Our study suggests the fine‐tuning mechanisms for SOP development by epigenetic methyltransferase and non‐apoptotic caspase function.
Type of Medium:
Online Resource
ISSN:
1356-9597
,
1365-2443
DOI:
10.1111/gtc.2016.21.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2020308-1
SSG:
12
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