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  • 1
    Online Resource
    Online Resource
    KARAKTERISASI BEBERAPA STRAIN GURAMI Osphronemus gouramy Lac. MENGGUNAKAN MARKA RAPD
    Universitas Jenderal Soedirman ; 2014
    In:  Scripta Biologica Vol. 1, No. 1 ( 2014-03-25), p. 109-
    Details
    In: Scripta Biologica, Universitas Jenderal Soedirman, Vol. 1, No. 1 ( 2014-03-25), p. 109-
    Abstract: Giant gouramy, Osphronemus gouramy Lac. is a popular fish species in Indonesia, especially in Java and Sumatera as this freshwater fish species has a high economic value of stable price. Fish farmers in Bogor divide giant gouramy into six strains based on egg productivity, growth rate, and maximum weight of the adult. They are soang, jepang, blue saphire, paris, bastar, and porselin. These various strains lead to the need of study on the genetic relationship among them, which can be performed by the use of RAPD (Random Amplified Polymorphic DNA) marker. This study aims to determine primers producing consistent and polymorphic RAPD markers, determine specific RAPD markers, and know the genetic relationship among several giant gouramy strains. The strains used in this study are soang, jepang, and blue saphire. Survey method was applied employing purposive random sampling technique. Total genomic DNA was isolated using Genjettm genomic DNA purification kit (Fermentas), which was then used as template to amplify RAPD markers with primers OPA-07, OPA-09, OPA-11, OPA-20, OPAH-01, OPAH-08, OPAH-09, and OPAC-14. The variables examined were patterns and numbers of specific DNA fragments as the PCR amplification products. Selected primers were determined descriptively on the basis of specific DNA bands appearing on the agarose gel. Genetic diversity was predetermined by changing qualitative band pattern into quantitative binnary data. Genetic relationship was analyzed using cladistic method with PAUP software. The results showed that only five of the eight primers produce consistent and polymorphic RAPD markers, i.e. OPA-11, OPA-20, OPAH-1, OPAH-8, and OPAH-9. Specific RAPD markers which can be used to distinguish several gouramy strains are those amplified with OPA-20 of 786 bp, OPA-20 of 1,176 bp, OPAH-8 of 1,000 bp and OPAC-14 of 1,607 bp. Nervertheless, it was found that RAPD markers cannot be used to clearly determine genetic relationship among gouramy strains.
    Type of Medium: Online Resource
    ISSN: 2355-3138
    URL: Article
    DOI: 10.20884/1.sb.2014.1.1.35
    Language: Unknown
    Publisher: Universitas Jenderal Soedirman
    Publication Date: 2014
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  • 2
    Online Resource
    Online Resource
    PREDIKSI CURAH HUJAN BULANAN UNTUK PERINGATAN DINI LONGSOR DI BANJARNEGARA BAGIAN SELATAN DENGAN STATISTICAL DOWNSCALING DAN ENSEMBLE
    Pusat Penelitian dan Pengembangan Badan Meteorologi Klimatologi dan Geofisika ; 2021
    In:  Jurnal Meteorologi dan Geofisika Vol. 21, No. 2 ( 2021-01-15), p. 69-
    Details
    In: Jurnal Meteorologi dan Geofisika, Pusat Penelitian dan Pengembangan Badan Meteorologi Klimatologi dan Geofisika, Vol. 21, No. 2 ( 2021-01-15), p. 69-
    Type of Medium: Online Resource
    ISSN: 2527-5372 , 1411-3082
    URL: Article
    DOI: 10.31172/jmg.v21i2.698
    Language: Unknown
    Publisher: Pusat Penelitian dan Pengembangan Badan Meteorologi Klimatologi dan Geofisika
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    High Connectivity Among Synedrella nodiflora Populations in Java Island Based on Intergenic Spacer atpB-rbcL
    Universitas Negeri Semarang ; 2018
    In:  Biosaintifika: Journal of Biology & Biology Education Vol. 10, No. 1 ( 2018-04-02), p. 41-47
    Details
    In: Biosaintifika: Journal of Biology & Biology Education, Universitas Negeri Semarang, Vol. 10, No. 1 ( 2018-04-02), p. 41-47
    Abstract: Synedrella nodiflora (L.) Gaertn has taxonomically been the only species of genus Synedrella, which spreads over many tropical countries. In spite of its wide range of distribution, the genus remains monospecific. This leads to assumption of the very low genetic diversity among S. nodiflora populations worldwide. It may also be the case in Java Island, though rapid changes in ecosystem condition occurs. Here we report our study on S. nodiflora population genetics in Java Island using intergenic spacer (IGS) atpB –  rbcL as a molecular marker, since it has been well known as one of the most variable chloroplast genome regions in a wide range of plant species so far. As many as 58 individuals were collected randomly from ten different locations in the island. Based on IGS atpB – rbcL sequences of 860 bp length, only two haplotypes were observed. Both show only one polymorphic site (0.12%) and one transversion, where T is substituted by G at position 790, indicating that high connectivity among populations of S. nodiflora in Java Island is observed. This results in a low genetic differences among the populations, which at the same time provides a fact of nearly no variation among the IGS atpB – rbcL sequences.
    Type of Medium: Online Resource
    ISSN: 2338-7610 , 2085-191X
    URL: Issue
    URL: Article
    DOI: 10.15294/biosaintifika.v10i1
    DOI: 10.15294/biosaintifika.v10i1.12038
    Language: Unknown
    Publisher: Universitas Negeri Semarang
    Publication Date: 2018
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  • 4
    Online Resource
    Online Resource
    Synedrella nodiflora (L.) Gaertn Populations in Sumatra Island Showed Low Genetic Differences: A study based on the intergenic spacer atpB – rbcL
    IOP Publishing ; 2020
    In:  IOP Conference Series: Earth and Environmental Science Vol. 593, No. 1 ( 2020-11-01), p. 012035-
    Details
    In: IOP Conference Series: Earth and Environmental Science, IOP Publishing, Vol. 593, No. 1 ( 2020-11-01), p. 012035-
    Abstract: Previous study on Synedrella nodiflora (L.) Gaertn populations in Java Island showed both very low haplotype and nucleotide diversity, and at the same time revealed high connectivity among the populations. Sumatra Island, which is like Java Island located in Sunda Shelf, has been subjected to relatively increasing human population and overexploitation of natural resources in a few last decades. This condition put the island of being vulnerable to terrestrial ecosystem changes that potentially influence the existing populations of S. nodiflora . Hence, this study aimed to assess genetic differences among S. nodiflora populations in Sumatra Island using intergenic spacer (IGS) atp B – rbc L. This molecular marker has been used in the population genetic study of some plant species. In this study we collected randomly 20 individuals from four different locations in Sumatra. The results showed, based on IGS atp B – rbc L sequences of 860 bp length, that only two haplotypes were found. One of them was the same haplotype mostly found in Java Island, and the other showed some base substitutions. Low genetic differences indicating high connectivity among populations of S. nodiflora in Sumatra Island is observed.
    Type of Medium: Online Resource
    ISSN: 1755-1307 , 1755-1315
    URL: Article
    DOI: 10.1088/1755-1315/593/1/012035
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2020
    detail.hit.zdb_id: 2434538-6
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  • 5
    Online Resource
    Online Resource
    Phylogeography and Genetic Diversity of Humpback Grouper Cromileptes altivelis based on Cytochrome C Oxidase I
    Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau ; 2012
    In:  Jurnal Natur Indonesia Vol. 14, No. 1 ( 2012-11-21), p. 47-
    Details
    In: Jurnal Natur Indonesia, Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau, Vol. 14, No. 1 ( 2012-11-21), p. 47-
    Abstract: Humpback grouper is one of the most popular fish group in the international live trade in Asia-Pacific regions. The price for one kilogramlive of humpback grouper, especially in Spermonde Archipelago South of Sulawesi, is range from 350.000-400.000 IDR, whereas in theretail level in Hong Kong the price was about 92 US$. This condition leads to the reduction of nature population due to overexploitation.Population decreasing due to overexploitation may cause loss of genetic diversity within population and lead to reduce of potentialadaptive, population resistance, and productivity. Therefore, it is important to do some efforts to avoid adverse effect of overexploitationon humpback grouper population in Indonesia. One of the valuable efforts is providing genetic information such as phylogeography andgenetic diversity of humpback grouper Cromileptes altivelis. Analysis was based on 618 base pairs fragment of cytochrome c oxidase I genefrom 36 individuals (sequences) of Cromileptes altivelis collected at four different sites (e.g. Pulau Seribu, Jepara, Situbondo and SpermondeArchipelago). The results showed that humpback grouper population has a high haplotype and nucleotide diversity. However, high geneticdiversity and polymorphisms could not reveal population fragmentation (Φ stt = 0.000). It is suggested that high gene flow rather thanpopulation sub structuring was occurred. High level genetic diversity and polymorphisms are vital related to adaptive potential toenvironmental alteration.
    Type of Medium: Online Resource
    ISSN: 2503-0345 , 1410-9379
    URL: Article
    DOI: 10.31258/jnat.14.1.47-51
    Language: Unknown
    Publisher: Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
    Publication Date: 2012
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  • 6
    Online Resource
    Online Resource
    Historical divergences associated with intermittent land bridges overshadow isolation by larval dispersal in co‐distributed species of Tridacna giant clams
    Wiley ; 2018
    In:  Journal of Biogeography Vol. 45, No. 4 ( 2018-04), p. 848-858
    Details
    In: Journal of Biogeography, Wiley, Vol. 45, No. 4 ( 2018-04), p. 848-858
    Abstract: The aim of this study was to test historical and contemporary influences on population structure in the giant clams, Tridacna maxima (Röding, 1798) and T. crocea (Lamarck, 1819). To refine the location of clade boundaries within a newly resurrected species, Tridacna noae (Röding, 1798). Location Indo‐Australian archipelago, including Indonesia, the Philippines, Australia, Papua New Guinea, the Solomon Islands, Republic of Kiribati, the Line Islands and Taiwan. Methods We used isolation‐migration ( IM a) coalescent models and distance‐based redundancy analyses (db RDA ) to test the relative influence of barriers and continuous distances on historical divergence, gene flow and population structure of T. maxima and T. crocea . Continuous metrics of distance included present‐day and Last Glacial Maximum overwater distances along with probability of larval dispersal (LD) among sampling sites. We combined new mitochondrial cytochrome oxidase subunit I (mt DNA COI ) sequences with existing data to compile the largest data set of these species yet analysed. Results The Pleistocene land barriers of the Sunda Shelf and Torres Strait were associated with old ( 〉 0.5 Myr) divergence times. The western and eastern boundaries of the Halmahera Eddy were also locations of significant, but more recent, divergence. No gene flow was detected across any of the four barriers tested. Larval dispersal distances between sampling sites were significant predictors of T. crocea population structure, accounting for differentiation above and beyond the contribution of barriers. We further delineated the species range of T. noae and showed that its two known clades are sympatric in central Indonesia. Main conclusions The strong signature of historical barriers on genetic differentiation argues against the assumption that Indo‐Pacific Tridacna are open meta‐populations. Despite similar life histories, T. maxima and T. crocea differ in their mt DNA population structure. The widespread species ( T. maxima ) exhibits population structure linked solely with historical factors, whereas T. crocea's population structure reflects both historical factors and LD distances.
    Type of Medium: Online Resource
    ISSN: 0305-0270 , 1365-2699
    URL: Issue
    URL: Article
    DOI: 10.1111/jbi.2018.45.issue-4
    DOI: 10.1111/jbi.13163
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2020428-0
    detail.hit.zdb_id: 188963-1
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains
    Universitas Negeri Semarang ; 2017
    In:  Biosaintifika: Journal of Biology & Biology Education Vol. 9, No. 3 ( 2017-12-31), p. 592-
    Details
    In: Biosaintifika: Journal of Biology & Biology Education, Universitas Negeri Semarang, Vol. 9, No. 3 ( 2017-12-31), p. 592-
    Abstract: 〈 p class="IsiAbstrakIndo" 〉 〈 span lang="EN-GB" 〉 Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was checked for giant gourami sequences available in GenBank. Kimura 2-Parameter genetic distances were calculated in MEGA6 software. Haplotype and nucleotide diversity within population and 〈 /span 〉 〈 span lang="EN-GB" 〉 Φ 〈 /span 〉 〈 sub 〉 〈 span lang="EN-GB" 〉 st 〈 /span 〉 〈 /sub 〉 〈 span lang="EN-GB" 〉 -value among populations were estimated in Arlequin software. Phylogenetic relationship was reconstructed using the neighbor-joining method in MEGA6 software based on Kimura 2-parameter model with 1000 pseudobootstraps. Taxonomic identification results in 99% sequences homology to 〈 /span 〉 〈 em 〉 〈 span lang="EN-GB" 〉 Osphronemus goramy 〈 /span 〉 〈 /em 〉 〈 span lang="EN-GB" 〉 sequences (accession number KU984978.1 and AY763768.1), means that all strains belong to single species. Low genetic distances, medium haplotype and low-level nucleotide diversity were observed among strains. Pairwise 〈 /span 〉 〈 span lang="EN-GB" 〉 Φ 〈 /span 〉 〈 sub 〉 〈 span lang="EN-GB" 〉 st 〈 /span 〉 〈 /sub 〉 〈 span lang="EN-GB" 〉 -comparison indicates no genetic differences among Sumatera strain, whereas strong genetic structures observed between Sumatera and Mandiangin strains. The phylogenetic tree showed that Mandiangin formed separate subclades from other strains with bootstraps value of 100%. This finding has important implication for breeding sciences and efforts. 〈 /span 〉 〈 /p 〉
    Type of Medium: Online Resource
    ISSN: 2338-7610 , 2085-191X
    URL: Issue
    URL: Article
    DOI: 10.15294/biosaintifika.v9i3
    DOI: 10.15294/biosaintifika.v9i3.12097
    Language: Unknown
    Publisher: Universitas Negeri Semarang
    Publication Date: 2017
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  • 8
    Online Resource
    Online Resource
    Molecular Characterization of Anguilla sp. with Special Notes on Its Population Genetic in the Rivers of Cilacap Central Java, Indonesia
    Universitas Negeri Semarang ; 2022
    In:  Biosaintifika: Journal of Biology & Biology Education Vol. 14, No. 1 ( 2022-04-13), p. 103-116
    Details
    In: Biosaintifika: Journal of Biology & Biology Education, Universitas Negeri Semarang, Vol. 14, No. 1 ( 2022-04-13), p. 103-116
    Abstract: Taxonomic status of Anguilla species in the rivers of Cilacap is vital for further study, such as population genetics and evolutionary history. Taxonomic status, population genetic, and evolutionary histories of Anguilla can be assessed using single genetic marker, such as cytochrome oxidase 1 gene. This study aimed to determine taxonomic status, genetic diversity and connectivity, and evolutionary history of Anguilla populations in the rivers of Cilacap. Anguilla samples were collected from Doplang-Serayu and Segara Anakan watersheds.  Sequence homology test to the conspecific sequence in GenBank proved that all samples from Doplang-Serayu watershed were genetically identified as Anguilla bicolor (98.23 to 100% homology). The decision was supported by monophyly between samples and their conspecific references. Anguilla bicolor from both watersheds had high haplotype (gene) diversity. Amova and Fst analysis proved that no genetic difference (p=0.623) was observed between Doplang-Serayu and Segara Anakan watersheds, indicated panmixing.  Haplotype network proved that both populations were evolved from two primitive ancestors. This study concluded that Anguilla bicolor was the only freshwater eel observed in the rivers of Cilacap. Anguilla bicolor in the rivers of Cilacap has high genetic diversity but no genetic differentiation was observed among populations. Anguilla bicolor population in the rivers of Cilacap evolved from two primitive ancestors.
    Type of Medium: Online Resource
    ISSN: 2338-7610 , 2085-191X
    URL: Issue
    URL: Article
    DOI: 10.15294/biosaintifika.v14i1
    DOI: 10.15294/biosaintifika.v14i1.35421
    Language: Unknown
    Publisher: Universitas Negeri Semarang
    Publication Date: 2022
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  • 9
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    Online Resource
    MORFOLOGI KEPITING Albunea symmysta (ALBUNEIDAE:CRUSTACEAE) DARI PANTAI PARANGKUSUMO YOGYAKARTA
    Universitas Jenderal Soedirman ; 2020
    In:  BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol. 2, No. 1 ( 2020-04-29), p. 91-
    Details
    In: BioEksakta : Jurnal Ilmiah Biologi Unsoed, Universitas Jenderal Soedirman, Vol. 2, No. 1 ( 2020-04-29), p. 91-
    Abstract: Familia Albuneidae terdiri atas Sembilan Genus dan salah satunya yaitu Albunea. Distribusi Albunea di Indonesia telah dilaporkan oleh beberapa peneliti terdahulu, diantaranya terdapat di pesisir Cilacap, Kebumen, Yogyakarta, Aceh, dan Bengkulu. Berdasarkan hasil survei pendahuluan yang dilakukan di Pantai Parangkusumo Yogyakarta telah ditemukan kepiting yang memiliki morfologi hampir sama dengan dengan spesies Albunea symmysta yang pernah ditemukan sebelumnya di lokasi yang berbeda. Atas dasar hal tersebut maka telah dilakukan kajian untuk mengetahui kepastian nama spesiesnya. Penelitian ini bertujuan untuk mengidentifikasi kepiting Albunea yang dimiliki oleh yang berasal dari pantai Parangkusumo Yogyakarta serta untuk mengetahui semua karakter performa dan  meristik milik kepiting Albunea yang berasal dari pantai Parangkusumo. Metode penelitian yang digunakan adalah survei dan pengambilan sampel secara purposive random sampling. Sampel dari pantai Parangkusumo diambil sebanyak dua kali dengan interval waktu dua minggu, pengambilan sampel dilakukan hingga memperoleh jumlah sampel sebanyak 60 ekor. Identifikasi dilakukan berdasarkan karakter morfologi, yang meliputi performa, morfometri dan meristik. Analisis data dilakukan secara deskriptif untuk performa dan meristik, sedangkan morfometrik dengan uji T. Berdasarkan pengamatan performa morfologi, meristik, hasil penelitian yang didapat yaitu kepiting Albunea yang diperoleh  dari Parangkusumo Yogyakarta adalah spesies Albunea symmysta yang memiliki karakter karapas dengan bentuk hampir empat persegi panjang, memiliki antenula yang sangat panjang, karapas dengan permukaan rata, memiliki dactylus pertama subchelate, memiliki 9-12 buah duri anterolateral dan memiliki tujuh buah segmen flagella antena  
    Type of Medium: Online Resource
    ISSN: 2714-8564
    URL: Article
    DOI: 10.20884/1.bioe.2020.2.1.1842
    Language: Unknown
    Publisher: Universitas Jenderal Soedirman
    Publication Date: 2020
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  • 10
    Online Resource
    Online Resource
    Pemberdayaan Kelompok Wanita Tani Desa Dawuhan Kulon Kabupaten Banyumas Melalui Knowlegde Transfer Pembenihan Ikan
    Universitas Nasional ; 2021
    In:  Populis : Jurnal Sosial dan Humaniora Vol. 6, No. 2 ( 2021-09-15), p. 121-135
    Details
    In: Populis : Jurnal Sosial dan Humaniora, Universitas Nasional, Vol. 6, No. 2 ( 2021-09-15), p. 121-135
    Abstract: Aktivitas kelompok wanita tani Desa Dawuhan Kulon Kecamatan Kedungbanteng Kabupaten Banyumas, selama ini adalah bercocok tanam padi, tanaman sayuran dan tanaman hias. Mulai tahun 2020 desa tersebut sedang menggalakkan program budidaya ikan, Kepala Desa menghendaki agar kelompok wanita tani terlibat dalam kegiatan itu. Tujuan pelatihan dan pendampingan ini adalah memberikan pengetahuan dan keterampilan kepada kelompok tersebut dalam pembenihan ikan Nila dan Nilem. Metode yang digunakan partisipatif yang direalisasikan dalam bentuk pelatihan, dengan tahapan  kegiatan: 1) persiapan, 2) pelaksanaan kegiatan (knowledge transfer, praktik, pembuatan demplot), 3) monitoring dan evaluasi, 4) pendampingan.  Hasil yang diperoleh adalah anggota kelompok wanita tani berhasil memproduksi benih ikan Nila dengan cara pemijahan alami dan memproduksi benih ikan Nilem melalui pijah induksi. Keberhasilan dalam memijahkan tersebut mengindikasikan bahwa anggota kelompok wanita tani di Desa Dawuhan Kulon  telah bertambah pengetahuan dan keterampilannya dalam melakukan pembenihan ikan air tawar.
    Type of Medium: Online Resource
    ISSN: 2549-7685 , 2460-4208
    URL: Issue
    URL: Article
    DOI: 10.47313/pjsh.v6i2
    DOI: 10.47313/pjsh.v6i2.1383
    Language: Unknown
    Publisher: Universitas Nasional
    Publication Date: 2021
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