In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 19, No. 6 ( 2023-6-22), p. e1010805-
Abstract:
Pelger-Huët anomaly (PHA) in humans is an autosomal dominant hematological phenotype without major clinical consequences. PHA involves a characteristic hyposegmentation of granulocytes (HG). Human PHA is caused by heterozygous loss of function variants in the LBR gene encoding lamin receptor B. Bi-allelic variants and complete deficiency of LBR cause the much more severe Greenberg skeletal dysplasia which is lethal in utero and characterized by massive skeletal malformation and gross fetal hydrops. HG phenotypes have also been described in domestic animals and homology to human PHA has been claimed in the literature. We studied a litter of Australian Shepherd Dogs with four stillborn puppies in which both parents had an HG phenotype. Linkage analysis excluded LBR as responsible gene for the stillborn puppies. We then investigated the HG phenotype in Australian Shepherd Dogs independently of the prenatal lethality. Genome-wide association mapped the HG locus to chromosome 27 and established an autosomal recessive mode of inheritance. Whole genome sequencing identified a splice site variant in LMBR1L , c.191+1G 〉 A, as most likely causal variant for the HG phenotype. The mutant allele abrogates the expression of the longer X2 isoform but does not affect transcripts encoding the shorter X1 isoform of the LMBR1L protein. The homozygous mutant LMBR1L genotype associated with HG is common in Australian Shepherd Dogs and was found in 39 of 300 genotyped dogs (13%). Our results point to a previously unsuspected function of LMBR1L in the myeloid lineage of leukocytes.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010805
DOI:
10.1371/journal.pgen.1010805.g001
DOI:
10.1371/journal.pgen.1010805.g002
DOI:
10.1371/journal.pgen.1010805.g003
DOI:
10.1371/journal.pgen.1010805.g004
DOI:
10.1371/journal.pgen.1010805.t001
DOI:
10.1371/journal.pgen.1010805.t002
DOI:
10.1371/journal.pgen.1010805.s001
DOI:
10.1371/journal.pgen.1010805.s002
DOI:
10.1371/journal.pgen.1010805.s003
DOI:
10.1371/journal.pgen.1010805.s004
DOI:
10.1371/journal.pgen.1010805.s005
DOI:
10.1371/journal.pgen.1010805.s006
DOI:
10.1371/journal.pgen.1010805.s007
DOI:
10.1371/journal.pgen.1010805.s008
DOI:
10.1371/journal.pgen.1010805.s009
DOI:
10.1371/journal.pgen.1010805.s010
DOI:
10.1371/journal.pgen.1010805.s011
DOI:
10.1371/journal.pgen.1010805.s012
DOI:
10.1371/journal.pgen.1010805.s013
DOI:
10.1371/journal.pgen.1010805.r001
DOI:
10.1371/journal.pgen.1010805.r002
DOI:
10.1371/journal.pgen.1010805.r003
DOI:
10.1371/journal.pgen.1010805.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2186725-2
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