In:
Biochemical Journal, Portland Press Ltd., Vol. 193, No. 3 ( 1981-03-01), p. 919-923
Abstract:
Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H] propionate (sp. radioactivity 50 Ci/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.
Type of Medium:
Online Resource
ISSN:
0264-6021
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
1981
detail.hit.zdb_id:
1473095-9
SSG:
12
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