In:
The Journal of Dermatology, Wiley, Vol. 40, No. 9 ( 2013-09), p. 726-730
Abstract:
S porotix schenckii is a pathogenic fungus that causes human and animal sporotrichosis, and based on morphology of the sessile conidia and molecular analysis, it was recently recognized as a species complex comprising at least the following six sibling species: S . albicans , S . brasiliensis , S . globosa , S . luriei, S . mexicana and S . schenckii . However, apart from S . schenckii sensu strict, only S . brasiliensis , S . globosa and S . luriei are associated with human and animal infection. S . globosa has been most commonly isolated in Asia, Europe and the USA; therefore, molecular epidemiological study for S . globosa is important in relation to human sporotrichosis in Japan. To the best of our knowledge, this is the first study to determine the mating type 1‐2 ( MAT1‐2 ) gene of S porothrix schenckii with the aim of understanding the taxonomy of the genus S porothrix . The MAT1‐2 gene (1618 bp) encodes a protein sequence of 198 amino acids. Reverse transcription polymerase chain reaction analysis also detected MAT1‐2 gene m RNA expression in all of the S . schenckii strains examined, indicating that this gene is expressed in S. schenckii cells. Phylogenetic analysis of the MAT1‐2 gene fragments of O phiostoma himal‐ulmi , O . novo‐ulmi , O . ulmi and S . schenckii indicated that these isolates could be classified into four clusters. MAT1‐1 gene‐specific polymerase chain reaction was positive in 15 isolates, but negative in four human isolates and one feline isolate.
Type of Medium:
Online Resource
ISSN:
0385-2407
,
1346-8138
DOI:
10.1111/jde.2013.40.issue-9
DOI:
10.1111/1346-8138.12226
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2222121-9
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