In:
European Journal of Immunology, Wiley, Vol. 36, No. 12 ( 2006-12), p. 3216-3226
Abstract:
Recently, we reported that Src homology 2 domain‐containing protein tyrosine phosphatase substrate 1 (SHPS‐1) plays an important role in the migration of Langerhans cells (LC). Here, we show that SHPS‐1 is involved in the maturation of LC. Immunofluorescence analysis on epidermal sheets for I‐A or CD86 revealed that LC maturation induced by 2,4‐dinitro‐1‐fluorobenzene (DNFB) or by TNF ‐ α was inhibited by pretreatment with an anti‐SHPS‐1 monoclonal antibody (mAb) or with CD47‐Fc fusion protein, a ligand for SHPS‐1. Further, FACS analysis demonstrated that I‐A + LC that had emigrated from skin explants expressed CD80 or CD86, whereas CD47‐Fc protein reduced CD80 high+ or CD86 high+ cells. CD47‐Fc protein also reduced the up‐regulation of surface CD80 or CD86 by LC remaining in the skin explants. In SHPS‐1 mutant mice, we observed that the up‐regulation of surface CD86 and CCR7 by LC induced by DNFB as well as that of surface CD80 and CD86 by LC in skin explants was attenuated. Finally, contact hypersensitivity (CHS) response was suppressed in SHPS‐1 mutant mice and in wild‐type mice treated with an anti‐SHPS‐1 mAb. These observations indicate that SHPS‐1 plays an important role in the maturation of LC ex vivo and in vivo , and that SHPS‐1‐CD47 interaction may negatively regulate CHS.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.200635864
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
1491907-2
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