In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-10-5)
Abstract:
Aegilops crassa Boiss . is polyploid grass species that grows in the eastern part of the Fertile Crescent, Afghanistan, and Middle Asia. It consists of tetraploid (4x) and hexaploid (6x) cytotypes (2 n = 4x = 28, D 1 D 1 X cr X cr and 2n = 6x = 42, D 1 D 1 X cr X cr D 2 D 2 , respectively) that are similar morphologically. Although many Aegilops species were used in wheat breeding, the genetic potential of Ae . crassa has not yet been exploited due to its uncertain origin and significant genome modifications. Tetraploid Ae . crassa is thought to be the oldest polyploid Aegilops species, the subgenomes of which still retain some features of its ancient diploid progenitors. The D 1 and D 2 subgenomes of Ae . crassa were contributed by Aegilops tauschii (2 n = 2x = 14, DD), while the X cr subgenome donor is still unknown. Owing to its ancient origin, Ae . crassa can serve as model for studying genome evolution. Despite this, Ae . crassa is poorly studied genetically and no genome sequences were available for this species. We performed low-coverage genome sequencing of 4x and 6x cytotypes of Ae . crassa , and four Ae . tauschii accessions belonging to different subspecies; diploid wheatgrass Thinopyrum bessarabicum (J b genome), which is phylogenetically close to D (sub)genome species, was taken as an outgroup. Subsequent data analysis using the pipeline RepeatExplorer2 allowed us to characterize the repeatomes of these species and identify several satellite sequences. Some of these sequences are novel, while others are found to be homologous to already known satellite sequences of Triticeae species. The copy number of satellite repeats in genomes of different species and their subgenome (D 1 or X cr ) affinity in Ae . crassa were assessed by means of comparative bioinformatic analysis combined with quantitative PCR (qPCR). Fluorescence in situ hybridization (FISH) was performed to map newly identified satellite repeats on chromosomes of common wheat, Triticum aestivum , 4x and 6x Ae . crassa , Ae . tauschii , and Th . bessarabicum . The new FISH markers can be used in phylogenetic analyses of the Triticeae for chromosome identification and the assessment of their subgenome affinities and for evaluation of genome/chromosome constitution of wide hybrids or polyploid species.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.980764
DOI:
10.3389/fpls.2022.980764.s001
DOI:
10.3389/fpls.2022.980764.s002
DOI:
10.3389/fpls.2022.980764.s003
DOI:
10.3389/fpls.2022.980764.s004
DOI:
10.3389/fpls.2022.980764.s005
DOI:
10.3389/fpls.2022.980764.s006
DOI:
10.3389/fpls.2022.980764.s007
DOI:
10.3389/fpls.2022.980764.s008
DOI:
10.3389/fpls.2022.980764.s009
DOI:
10.3389/fpls.2022.980764.s010
DOI:
10.3389/fpls.2022.980764.s011
DOI:
10.3389/fpls.2022.980764.s012
DOI:
10.3389/fpls.2022.980764.s013
DOI:
10.3389/fpls.2022.980764.s014
DOI:
10.3389/fpls.2022.980764.s015
DOI:
10.3389/fpls.2022.980764.s016
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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