In:
Journal of Basic Microbiology, Wiley, Vol. 61, No. 3 ( 2021-03), p. 219-229
Abstract:
Multi‐replicon plasmids harboring the Inc pA1763‐KPC replicon together with other replicons are being increasingly reported among Enterobacteriaceae species. However, plasmids with single Inc pA1763‐KPC replicons are poorly studied as a different incompatibility (Inc) group, despite their rise in appearance in some strains. Inc pA1763‐KPC plasmids, pA1763‐KPC, and p427113‐2, from two clinical Klebsiella pneumoniae isolates were fully sequenced by high‐throughput genome sequencing. Linear structural comparisons of Inc pA1763‐KPC backbone region were made between these two plasmids and six arbitrarily selected representative Inc pA1763‐KPC plasmids sequenced previously. A further detailed genomic comparison was carried out between plasmids pA1763‐KPC, p427113‐2, and pFB2.2, which show high homology across the backbone sequence to one another. Among all sequenced Inc pA1763‐KPC plasmids considered in this study, plasmids pA1763‐KPC and p427113‐2 showed the most complete Inc pA1763‐KPC backbones. These were composed of the Inc pA1763‐KPC replicon ( repA IncpA1763‐KPC and its iterons), the 5.6‐kb Inc pA1763‐KPC ‐type maintenance region, the 27.7‐kb IncFII K ‐type maintenance region, and the 36.6‐kb IncFII K ‐type conjugal transfer regions. Compared with pA1763‐KPC or p427113‐2, the backbone regions of the other analyzed Inc pA1763‐KPC plasmids had gradually undergone different deletions or truncations, but shared small and core Inc pA1763‐KPC backbones including the Inc pA1763‐KPC replicon, Inc pA1763‐KPC ‐type maintenance region, and residual IncFII K ‐type maintenance region. Accessory modules integrated into Inc pA1763‐KPC backbones included the multidrug‐resistant module bla KPC‐2 region in pA1763‐KPC, the metal‐resistance modules ars region together with ncr region in pFB2.2 and sil in pKPN‐9a0d, the IS Kpn14 ‐to‐IS 26 region in p427113‐2, and other non‐resistance region in the respective plasmids. This detailed comparative genomics analysis of Inc pA1763‐KPC plasmids provides a deep insight into their diversification and evolution.
Type of Medium:
Online Resource
ISSN:
0233-111X
,
1521-4028
DOI:
10.1002/jobm.202000668
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
1480967-9
detail.hit.zdb_id:
632513-0
detail.hit.zdb_id:
203025-1
SSG:
12
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