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  • 1
    Online Resource
    Online Resource
    Genetic profiling of Vietnamese population from large-scale genomic analysis of non-invasive prenatal testing data
    Springer Science and Business Media LLC ; 2020
    In:  Scientific Reports Vol. 10, No. 1 ( 2020-11-05)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-11-05)
    Abstract: The under-representation of several ethnic groups in existing genetic databases and studies have undermined our understanding of the genetic variations and associated traits or diseases in many populations. Cost and technology limitations remain the challenges in performing large-scale genome sequencing projects in many developing countries, including Vietnam. As one of the most rapidly adopted genetic tests, non-invasive prenatal testing (NIPT) data offers an alternative untapped resource for genetic studies. Here we performed a large-scale genomic analysis of 2683 pregnant Vietnamese women using their NIPT data and identified a comprehensive set of 8,054,515 single-nucleotide polymorphisms, among which 8.2% were new to the Vietnamese population. Our study also revealed 24,487 disease-associated genetic variants and their allele frequency distribution, especially 5 pathogenic variants for prevalent genetic disorders in Vietnam. We also observed major discrepancies in the allele frequency distribution of disease-associated genetic variants between the Vietnamese and other populations, thus highlighting a need for genome-wide association studies dedicated to the Vietnamese population. The resulted database of Vietnamese genetic variants, their allele frequency distribution, and their associated diseases presents a valuable resource for future genetic studies.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-020-76245-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2615211-3
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  • 2
    Online Resource
    Online Resource
    Detection of maternal carriers of common α-thalassemia deletions from cell-free DNA
    Springer Science and Business Media LLC ; 2022
    In:  Scientific Reports Vol. 12, No. 1 ( 2022-08-09)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2022-08-09)
    Abstract: α-Thalassemia is a common inherited blood disorder manifested mainly by the deletions of α-globin genes. In geographical areas with high carrier frequencies, screening of α-thalassemia carrier state is therefore of vital importance. This study presents a novel method for identifying female carriers of common α-thalassemia deletions using samples routinely taken for non-invasive prenatal tests for screening of fetal chromosomal aneuploidies. A total of 68,885 Vietnamese pregnant women were recruited and α-thalassemia statuses were determined by gap-PCR, revealing 5344 women (7.76%) carried deletions including αα/−− SEA (4.066%), αα/−α 3.7 (2.934%), αα/−α 4.2 (0.656%), and rare genotypes (0.102%). A two-stage model was built to predict these α-thalassemia deletions from targeted sequencing of the HBA gene cluster on maternal cfDNA. Our method achieved F1-scores of 97.14–99.55% for detecting the three common genotypes and 94.74% for detecting rare genotypes (−α 3.7 /−α 4.2 , αα/−− THAI , −α 3.7 /−− SEA , −α 4.2 /−− SEA ). Additionally, the positive predictive values were 100.00% for αα/αα, 99.29% for αα/−− SEA , 94.87% for αα/−α 3.7 , and 96.51% for αα/−α 4.2 ; and the negative predictive values were 97.63%, 99.99%, 99.99%, and 100.00%, respectively. As NIPT is increasingly adopted for pregnant women, utilizing cfDNA from NIPT to detect maternal carriers of common α-thalassemia deletions will be cost-effective and expand the benefits of NIPT.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-022-17718-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2615211-3
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  • 3
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    Online Resource
    Antibiotic resistance and heteroresistance in Helicobacter pylori isolates from symptomatic Vietnamese children: A prospective multicenter study
    Wiley ; 2023
    In:  Helicobacter Vol. 28, No. 5 ( 2023-10)
    Details
    In: Helicobacter, Wiley, Vol. 28, No. 5 ( 2023-10)
    Abstract: Antibiotic resistance of Helicobacter pylori (H. pylori) is increasing worldwide, with geographical variations, impacting the treatment outcomes. This study assessed the antibiotic resistance patterns of H. pylori in Vietnamese children. Materials and Methods Symptomatic children undergoing gastroduodenoscopy at two tertiary Children's Hospitals in Ho Chi Minh City were recruited. Antral and corpus biopsies were obtained and cultured separately. Susceptibility to amoxicillin (AMO), clarithromycin (CLA), metronidazole (MET), levofloxacin (LEV), and tetracycline (TET) was determined using E‐test. Polymerase chain reaction was performed on another antral biopsy to detect the urease gene, cytotoxin‐associated gene A ( cagA ), vacuolating cytotoxin A ( vacA ) genotypes, and 23S rRNA mutations conferring CLA resistance. Results Among 123 enrolled children, a high primary resistance rate was found for CLA (68.5%, 61/89), followed by LEV (55.1%), MET (31.5%), AMO (25.8%), and TET (1.1%). Secondary resistance rates were 82.1% (7/28), 71.4%, 53.6%, and 3.6% for CLA, LEV, MET, and TET, respectively. Multidrug resistance was frequent (67.7%), with common patterns including CLA + LEV (20.3%) and CLA + MTZ + LEV (15.2%). Heteroresistance was detected in eight children (6.5%). The A2143G mutation was detected in 97.5% (119/122) of children. 86.1% of children had positive cagA strains and 27.9% had multiple vacA genotypes. No factor was significantly associated with antibiotic resistance. Conclusions The alarming rate of antibiotic resistance for H. pylori , especially for CLA, with emerging multi‐ and hetero‐resistant strains, pose a major treatment challenge that precludes CLA use as empirical therapy. Biopsies from both antrum and corpus can improve H. pylori culture, allowing tailored treatment based on antimicrobial susceptibility.
    Type of Medium: Online Resource
    ISSN: 1083-4389 , 1523-5378
    URL: Issue
    URL: Article
    DOI: 10.1111/hel.v28.5
    DOI: 10.1111/hel.13009
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020336-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Evaluation of general understanding on genetics subject of high school students
    Journal of Science, Hanoi National University of Education ; 2021
    In:  Journal of Science Educational Science Vol. 66, No. 4G ( 2021-11), p. 17-24
    Details
    In: Journal of Science Educational Science, Journal of Science, Hanoi National University of Education, Vol. 66, No. 4G ( 2021-11), p. 17-24
    Abstract: Genetics is one of the important topics in the high school biology curriculum. Genetics has been taught in the subject of biology at grade 9 and grade 12. To investigate the general understanding of genetics of high school students, a survey was carried out and got responses from participants of 901 high school students across the country. The data for the average score was 3.3; median 3.0; scores ranging from 0.33 to 8.33. The data analyses showed that there was no significant difference in the results between urban and rural regions. The general knowledge of genetics of high school students was low. In general, scores of students in gifted schools were higher than in non-gifted schools. In the group of gifted schools, the biological group gave higher results than others. In general, the students tend to choose the subject Biology and have high average scores, also got survey scores higher than those of other groups. Those results contribute to clarifying the reality of learning Biology in general and Genetics in particular of high school students, contributing to the basis for educational administrators to provide more appropriate educational policies.
    Type of Medium: Online Resource
    ISSN: 2354-1075
    Uniform Title: ĐÁNH GIÁ NHẬN THỨC CHUNG VỀ PHẦN DI TRUYỀN HỌC CỦA HỌC SINH TRUNG HỌC PHỔ THÔNG
    URL: Journal
    URL: Article
    DOI: 10.18173/2354-1075
    DOI: 10.18173/2354-1075.2021-0166
    Language: Unknown
    Publisher: Journal of Science, Hanoi National University of Education
    Publication Date: 2021
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  • 5
    Online Resource
    Online Resource
    Purification of CD34+ cells in myasthenia gravis patient’s peripheral blood stem cells using the CliniMACS cell separation system
    108 Institute of Clinical Medical and Pharmaceutical Sciences ; 2021
    In:  Journal of 108 - Clinical Medicine and Phamarcy Vol. 16, No. TA ( 2021-12-13)
    Details
    In: Journal of 108 - Clinical Medicine and Phamarcy, 108 Institute of Clinical Medical and Pharmaceutical Sciences, Vol. 16, No. TA ( 2021-12-13)
    Abstract: Autologous hematopoietic stem cell transplantation therapy has been widely used in recent decades for the treatment of hematological diseases, cancer, and autoimmune diseases. In autoimmune diseases, self-activated T lymphocytes and B lymphocytes secreting antibodies against host antigens are considered to be central in the pathogenesis of the disease. Autologous stem cell products that eliminates these pathological cells could be a lifesaver for patients with autoimmune diseases who are resistant to conventional therapy, in order to restore a healthy immune system, achieve long-term remission and limited recurrence. The aims of this study was to complete and evaluate the results of the CD34 positive stem cells purification procedure from a myasthenia gravis patient’s mobilized peripheral blood stem cells, for the purpose of autologous hematopoietic stem cell transplantation for Myasthenia gravis as well as other autoimmune diseases in Vietnam. We have completed the purification procedure of CD34-positive stem cells from mobilized peripheral blood stem cells of patient with myasthenia gravis using CliniMACS system with results of removing 99.99% of T lymphocytes; 99.81% of B lymphocytes; 99.99% of NK cells, while CD34+ recovery performance was 64.05% with CD34+ cell survival rate of over 99%, no bacterial contamination, ensuring quality assurance of stem cell product for transplantation.
    Type of Medium: Online Resource
    ISSN: 1859-2872
    URL: Issue
    URL: Article
    DOI: 10.52389/ydls.v16iTA
    DOI: 10.52389/ydls.v16iTA.1123
    Language: Unknown
    Publisher: 108 Institute of Clinical Medical and Pharmaceutical Sciences
    Publication Date: 2021
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  • 6
    Online Resource
    Online Resource
    Emergence of amoxicillin resistance and identification of novel mutations of the pbp1A gene in Helicobacter pylori in Vietnam
    Springer Science and Business Media LLC ; 2022
    In:  BMC Microbiology Vol. 22, No. 1 ( 2022-02-03)
    Details
    In: BMC Microbiology, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-02-03)
    Abstract: Amoxicillin-resistant Helicobacter pylori ( H. pylori ) strains seem to have increased over time in Vietnam. This threatens the effectiveness of H. pylori eradication therapies with this antibiotic. This study aimed to investigate the prevalence of primary resistance of H. pylori to amoxicillin and to assess its association with pbp1A point mutations in Vietnamese patients. Materials and methods Naive patients who presented with dyspepsia undergoing upper gastrointestinal endoscopy were recruited. Rapid urease tests and PCR assays were used to diagnose H. pylori infection. Amoxicillin susceptibility was examined by E-tests. Molecular detection of the mutant pbp1A gene conferring amoxicillin resistance was carried out by real-time PCR followed by direct sequencing of the PCR products. Phylogenetic analyses were performed using the Tamura-Nei genetic distance model and the neighbor-joining tree building method. Results There were 308 patients (46.1% men and 53.9% women, p  = 0.190) with H. pylori infection. The mean age of the patients was 40.5 ± 11.4 years, ranging from 18 to 74 years old. The E-test was used to determine the susceptibility to amoxicillin (minimum inhibitory concentration (MIC) ≤ 0.125 μg/ml) in 101 isolates, among which the rate of primarily resistant strains to amoxicillin was 25.7%. Then, 270 sequences of pbp1A gene fragments were analysed. There were 77 amino acid substitution positions investigated, spanning amino acids 310–596, with the proportion varying from 0.4 to 100%. Seven amino acid changes were significantly different between amoxicillin-sensitive (Amox S ) and amoxicillin-resistant (Amox R ) samples, including Phe 366 to Leu ( p   〈   0.001), Ser 414 to Arg ( p   〈   0.001), Glu/Asn 464–465 ( p  = 0.009), Val 469 to Met ( p  = 0.021), Phe 473 to Val ( p   〈   0.001), Asp 479 to Glu ( p  = 0.044), and Ser/Ala/Gly 595–596 ( p  = 0.001). Phylogenetic analyses suggested that other molecular mechanisms might contribute to amoxicillin resistance in H. pylori in addition to the alterations in PBP1A. Conclusions We reported the emergence of amoxicillin-resistant Helicobacter pylori strains in Vietnam and new mutations statistically associated with this antimicrobial resistance. Additional studies are necessary to identify the mechanisms contributing to this resistance in Vietnam.
    Type of Medium: Online Resource
    ISSN: 1471-2180
    URL: Article
    DOI: 10.1186/s12866-022-02463-8
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2041505-9
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish 〈i〉Amphiprion ocellaris〈/i〉 (Cuvier, 1830)
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Tạp chí Khoa học và Công nghệ biển Vol. 20, No. 4A ( 2021-04-11), p. 163-172
    Details
    In: Tạp chí Khoa học và Công nghệ biển, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 20, No. 4A ( 2021-04-11), p. 163-172
    Abstract: This study was designed to evaluate the effects of astaxanthin in broodfish diets on reproductive performance, egg quality and larvae quality parameters of clownfish (Amphirion ocellaris). Five treatments were tested with 5 levels of astaxanthin (Carophyll Pink 10% CWS) of 0, 50, 100, 150 and 200 mg/kg added to the feed. Each treatment was repeated in triplicate and the supplemental feeding trials were arranged for 13 months. The results showed that there were significant differences in hatching rate of egg, malformed rate and survival rate of larvae in 3 days post-hatch (p 〈 0.05) among the feeding trials of astaxanthin supplements. The highest hatching rate of egg and survival rate and the lowest malformed rate of larvae were observed in the treatment that was supplemented with astaxanthin 150 mg/kg feed, respectively 92.14 %; 93.57 % and 0.55 %. However, the astaxanthin supplementary diets did not affect the re-maturation and spawning period, spawning frequency, fecundity, egg diameter and larval size of nemo fish among the treatments. The results also suggested that astaxanthin requirement for clownfish broodstock to improve reproductive performance was 150 mg/kg feed.
    Type of Medium: Online Resource
    ISSN: 1859-3097
    URL: Article
    DOI: 10.15625/1859-3097/15644
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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  • 8
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    Online Resource
    Phosphorus treatment in wastewater by microorganisms isolated from cassava starch production waste : Nghiên cứu khả năng xử lý photpho trong nước thải của vi sinh vật phân lập từ nguồn thải của quá trình sản xuất tinh bột sắn
    Sachsische Landesbibliothek, Staats- und Universitatsbibliothek Dresden ; 2018
    In:  Journal of Vietnamese Environment Vol. 9, No. 3 ( 2018-07-20), p. 141-144
    Details
    In: Journal of Vietnamese Environment, Sachsische Landesbibliothek, Staats- und Universitatsbibliothek Dresden, Vol. 9, No. 3 ( 2018-07-20), p. 141-144
    Abstract: In waste water, phosphorous (P) can exist in inorganic or organic forms. Depending on the concentration, P can cause eutrophication and severe environmental pollution. Microorganisms have the ability to use and accumulate P, so microorganisms are studied to treat P in waste water in general and wastewater from cassava starch processing plants in particular. Research results show that in the 20 samples of waste water and sludge of the plant has selected three strains of bacteria that can accumulate P in the form of granules in the cell. Among them, SHV22 has the highest P accumulation capacity, reaching 3.05x10-11 mg/cell, P removal efficiency in wastewater from cassava starch processing factory is 82.1%. The strain was identified as Bacillus amyloliquefaciens. Trong nước thải P có thể tồn tại dưới dạng vô cơ hoặc hữu cơ. Tùy thuộc vào nồng độ, P có thể gây phú dưỡng và ô nhiễm môi trường nghiêm trọng. Vi sinh vật có khả năng sử dụng và tích lũy P, do đó vi sinh vật là đối tượng được nghiên cứu để xử lý P trong nước thải nói chung và nước thải của nhà máy chế biến tinh bột sắn nói riêng. Kết quả nghiên cứu cho thấy, trong 20 mẫu nước và bùn thải của nhà máy đã chọn lựa được 3 chủng vi khuẩn có khả năng tích lũy P dưới dạng hạt trong tế bào. Trong số đó, chủng SHV22 có khả năng tích lũy P cao nhất, đạt tới 3,05x10-11 mg/tế bào, hiệu quả loại bỏ P trong nước thải của nhà máy chế biến tinh bột sắn đạt 82,1%. Chủng đã được định danh là Bacillus amyloliquefaciens.
    Type of Medium: Online Resource
    ISSN: 2193-6471
    URL: Issue
    URL: Article
    DOI: 10.13141/jve.vol9.no3
    DOI: 10.13141/jve.vol9.no3.pp141-144
    Language: Unknown
    Publisher: Sachsische Landesbibliothek, Staats- und Universitatsbibliothek Dresden
    Publication Date: 2018
    detail.hit.zdb_id: 2674408-9
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  • 9
    Online Resource
    Online Resource
    Effects of dietary Mannan oligosaccharide (MOS) on grown, survival rate, intestinal morphology and blood cell count of the golden trevally fish (〈i〉Gnathanodon specious〈/i〉)
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2019
    In:  Tạp chí Khoa học và Công nghệ biển Vol. 19, No. 4A ( 2019-11-08), p. 241-250
    Details
    In: Tạp chí Khoa học và Công nghệ biển, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4A ( 2019-11-08), p. 241-250
    Abstract: The golden trevally fishes (Gnathanodon specious) (2.19 ± 0.23 g) were cultured in glass tanks with density of 20 fishes/tank and they were fed supplemental diets of different MOS concentrations (0; 0.2; 0.4 and 0.6%) for 90 days. Collected data included growth rate, survival rate and some hematological characteristics of this fish. The results demonstrated that MOS supplementation did not affect growth performance, erythrocyte density and blood cell size, however the survival rate was significantly increased. On the other hand, the total number of white blood cells (BC) on the 60th day in the fish fed with MOS supplements (5.78–6.96 × 104TB/mm3) was higher than that in the control group (only 5.43 × 104TB/mm3) with the largest total leukocytes (6.96 ± 0.50 × 104TB /mm3) at 0.2% MOS (p 〈 0.05).
    Type of Medium: Online Resource
    ISSN: 1859-3097
    URL: Article
    DOI: 10.15625/1859-3097/19/4A/14600
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2019
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  • 10
    Online Resource
    Online Resource
    EFFECT OF DIETARY VITAMIN E ON REPRODUCTIVE PERFORMANCE, EGG QUALITY AND LARVAE OF CLOWNFISH Amphiprion ocellaris (CUVIER, 1830)
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2019
    In:  Vietnam Journal of Marine Science and Technology Vol. 18, No. 4A ( 2019-03-14), p. 165-173
    Details
    In: Vietnam Journal of Marine Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 4A ( 2019-03-14), p. 165-173
    Abstract: This study was carried out to determine the effects of vitamin E (a-tocopherol) in five levels (0, 125, 250, 375 and 500 mg vitamin E/kg feed) in broodfish diets on reproductive, egg and larval quality parameters of clownfish (Amphirion ocellaris). Each treatment was repeated in triplicate and the supplemental feeding trial was arranged for 13 months. The result showed that there were no significant differences in re-maturation and spawning periods, spawning frequency, fecundity, egg diameter and larval size of Nemo fish observed between the treatments. However, diets supplemented with vitamin E positively influenced the rate of egg loss, hatching rate of egg and survival rate of the 3 days post hatch. The overall result of this experiment indicated that the optimum vitamin E requirement of clownfish for reproductive performance was 375 mg vitamin E/kg feed.
    Type of Medium: Online Resource
    ISSN: 1859-3097
    URL: Article
    DOI: 10.15625/1859-3097/18/4A/13644
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2019
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