In:
The Plant Journal, Wiley, Vol. 111, No. 2 ( 2022-07), p. 546-566
Abstract:
In cereals, the root system is mainly composed of post‐embryonic shoot‐borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to the ASYMMETRIC LEAVES2‐LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, is a key regulator of crown root initiation in rice ( Oryza sativa ). Here, we show that CRL1 can bind, both in vitro and in vivo , not only the LBD‐box , a DNA sequence recognized by several ASL/LBD transcription factors, but also another not previously identified DNA motif that was named CRL1‐box . Using rice protoplast transient transactivation assays and a set of previously identified CRL1‐regulated genes, we confirm that CRL1 transactivates these genes if they possess at least a CRL1‐box or an LBD‐box in their promoters. In planta , ChIP‐qPCR experiments targeting two of these genes that include both a CRL1‐ and an LBD‐box in their promoter show that CRL1 binds preferentially to the LBD‐box in these promoter contexts . CRISPR/Cas9‐targeted mutation of these two CRL1‐regulated genes, which encode a plant Rho GTPase ( OsROP ) and a basic helix–loop–helix transcription factor ( OsbHLH044 ), show that both promote crown root development. Finally, we show that OsbHLH044 represses a regulatory module, uncovering how CRL1 regulates specific processes during crown root formation.
Type of Medium:
Online Resource
ISSN:
0960-7412
,
1365-313X
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2020961-7
SSG:
12
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