In:
Zygote, Cambridge University Press (CUP), Vol. 22, No. 2 ( 2014-05), p. 165-173
Abstract:
The aim of the present study was to examine the effects of fetal bovine serum (FBS) concentration, equilibration time, and oocyte pre-treatment with cytochalasin B (CCB) on subsequent development of vitrified-warmed ovine immature (GV COCs ) and matured (MII) oocytes with (MII COCs ) or without cumulus cells (MII DOs ). In Experiment 1, the effects of FBS concentrations (10 and 20%) during the vitrification-warming procedure were examined. Survival rates after warming were not different between GV COCs , MII COCs and MII DOs oocytes. After in vitro fertilization, rate of cleaved embryos in MII COCs group at the presence of 20%FBS was higher than MII DOs and GV COCs groups. In Experiment 2, the effects of equilibration times (5, 7, and 10 min) were examined. There was no difference in survival rate of vitrified-warmed oocytes equilibrated at different times. Although, the rate of cleavage in MII COCs and MII DOs oocytes equilibrated for 10 and 7 min, respectively, was higher than 5 min equilibrated MII DOs and 7 and 10 min equilibrated GV COCs oocytes. In Experiment 3, the effects of oocyte pre-treatment with CCB were examined. Despite the insignificant difference in survival rate of vitrified-warmed ovine immature and matured oocytes, the rates of cleavage in CCB pretreated groups were significantly lower than untreated groups. Moreover, the blastocysts were only derived from those cumulus enclosed vitrified-warmed germinal vesicle (GV) and MII oocytes that had been exposed to 10% FBS in the absence of CCB. In conclusion, the presence of cumulus cells, 10% FBS, and the omission of CCB were beneficial for post-warming development of vitrified ovine oocytes.
Type of Medium:
Online Resource
ISSN:
0967-1994
,
1469-8730
DOI:
10.1017/S096719941200038X
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2014
detail.hit.zdb_id:
1483381-5
SSG:
12
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