In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 14, No. 12 ( 2020-12-28), p. e0008998-
Abstract:
Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C . sinensis -infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini -infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008998
DOI:
10.1371/journal.pntd.0008998.g001
DOI:
10.1371/journal.pntd.0008998.g002
DOI:
10.1371/journal.pntd.0008998.g003
DOI:
10.1371/journal.pntd.0008998.g004
DOI:
10.1371/journal.pntd.0008998.g005
DOI:
10.1371/journal.pntd.0008998.s001
DOI:
10.1371/journal.pntd.0008998.s002
DOI:
10.1371/journal.pntd.0008998.s003
DOI:
10.1371/journal.pntd.0008998.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2429704-5
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