In:
Pathobiology, S. Karger AG, Vol. 69, No. 2 ( 2001), p. 86-95
Abstract:
Interleukin 15 (IL-15 mRNA expression was detected in human colorectal cancer cells (Colo320, WiDr, TCO and DLD1) by the reverse transcriptase-polymerase chain reaction (RT-PCR). Only Colo320 and WiDr cells secreted IL-15 culture medium. With IL-15 treatment, all cell lines grew at a rate of 120–180% of that of nontreated cells. A binding assay with 〈 sup 〉 125 〈 /sup 〉 I-labeled IL-15 showed binding activity to IL-15 in Colo320 (K 〈 sub 〉 d 〈 /sub 〉 : 0.098 n 〈 i 〉 M 〈 /i 〉 ) cells. IL-15 also reversed the growth inhibition caused by serum starvation in Colo320 cells. IL-15-induced cell growth in regular and serum-free media was abrogated by anti-IL-15 antibody treatment in Colo320 cells. Moreover, IL-15 treatment reduced doxorubicin-induced cytostasis and cytolysis in Colo320 cells by 50%. The invasion capacity of IL-15-treated Colo320 cells was 5.3 times that of untreated cells. Immunoblotting showed that IL-15-treated Colo320 cells exhibited downregulation of p21Waf1 and Bax, and upregulation of Bcl-2, phospho-AKT, MMP9/MMP2, and VEGF. Finally, immunostaining of human colon cancer revealed that 33 (70%) of 47 Dukes’ C cases showed IL-15 expression in cancer cells, whereas only 16% of Dukes’ B cases did (p 〈 0.0001). IL-15 may play important roles in cell proliferation, invasion, and metastasis of human colorectal cancer.
Type of Medium:
Online Resource
ISSN:
1015-2008
,
1423-0291
Language:
English
Publisher:
S. Karger AG
Publication Date:
2001
detail.hit.zdb_id:
1483541-1
SSG:
12
Permalink