In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_6 ( 2013-06-01), p. 2019-2024
Abstract:
A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium -like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370 T (98.4 % 16S rRNA gene sequence similarity), A. canis P6775 T (97.4 %), A. phocae DSM 10002 T (97.4 %), A. pluranimalium M430/94/2 T (95.7 %) and A. hippocoleae CCUG 44697 T (95.5 %). The presence of the major menaquinone MK-9(H 4 ) supported the affiliation of the isolates with the genus Arcanobacterium . The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C 16 : 0 , C 18 : 0 , C 18 : 1 ω9 c and summed feature 5 (comprising C 18 : 2 ω6,9 c and/or anteiso-C 18 : 0 ). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium . Based on the common origin and various physiological properties comparable to those of A. phocae , it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698 T ( = LMG 27073 T = CCM 8430 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.045591-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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