In:
Immunology, Wiley, Vol. 146, No. 1 ( 2015-09), p. 59-69
Kurzfassung:
To clarify the effect of secretory IgA (s I gA) deficiency on gut homeostasis, we examined intraepithelial lymphocytes ( IEL s) in the small intestine ( SI ) of polymeric immunoglobulin receptor‐deficient ( pIgR −/− ) mice. The p I gR −/− mice exhibited the accumulation of CD 8 αβ + T ‐cell receptor ( TCR )‐ αβ + IEL s ( CD 8 αβ + αβ ‐ IEL s) after weaning, but no increase of CD 8 αβ + γδ ‐ IEL s was detected in pIgR −/− TCR ‐ β −/− mice compared with pIgR +/+ TCR ‐ β −/− mice. When 5‐bromo‐2′‐deoxyuridine (BrdU) was given for 14 days, the proportion of BrdU‐labelled cells in SI ‐ IEL s was not different between pIgR +/+ mice and pIgR −/− mice. However, the proportion of BrdU‐labelled CD 8 αβ + ‐ IEL s became higher in pIgR −/− mice than pIgR +/+ mice 10 days after discontinuing BrdU‐labelling. Intravenously transferred splenic T cells migrated into the intraepithelial compartments of pIgR +/+ TCR ‐ β −/− mice and pIgR −/− TCR ‐ β −/− mice to a similar extent. In contrast, in the case of injection of immature bone marrow cells, CD 8 αβ + αβ ‐ IEL s increased much more in the SI of pIgR −/− TCR ‐ β −/− mice than pIgR +/+ TCR ‐ β −/− mice 8 weeks after the transfer. αβ ‐ IEL s from pIgR −/− mice could produce more interferon‐ γ and interleukin‐17 than those of pIgR +/+ mice, and intestinal permeability tended to increase in the SI of pIgR −/− mice with aging. Taken together, these results indicate that activated CD 8 αβ + αβ ‐ IEL s preferentially accumulate in pIgR −/− mice through the enhanced differentiation of immature haematopoietic precursor cells, which may subsequently result in the disruption of epithelial integrity.
Materialart:
Online-Ressource
ISSN:
0019-2805
,
1365-2567
DOI:
10.1111/imm.2015.146.issue-1
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2015
ZDB Id:
2006481-0
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