In:
Israel Journal of Chemistry, Wiley, Vol. 53, No. 6-7 ( 2013-06), p. 417-430
Kurzfassung:
Nanopore technology holds high potential for next‐generation DNA sequencing. This method operates by drawing an individual single‐stranded DNA molecule through a nanoscale pore, while monitoring the current deflections that occur as the DNA passes through. Individual current levels for the four DNA nucleotides have been established by immobilization of an end biotinylated strand in the pore, in which the nucleotide of interest is suspended at the most sensitive region of the ion channel. Due to the inherent reactivity of DNA bases, many modified nucleotides in the genome exist as a result of oxidative and UV insults, among others. Herein, the current levels for common DNA damage lesions 8‐oxo‐7,8‐dihydroguanine, spiroiminodihydantoin, guanidinohydantoin, uridine, abasic sites, thymine dimers, thymine glycol, and 5‐iodocytosine were assessed through immobilization experiments. In some cases, the current difference between the damaged and canonical nucleotides was not well resolved; therefore, we took advantage of the chemical reactivity of the new functional groups present to make amine adducts that shifted the current levels outside the range of the native nucleotides. Among the adducts studied, only the 2‐aminomethyl‐18‐crown‐6 adduct was able to give a large current shift in the immobilization experiment, as well as being observed in a translocation experiment. The results show potential in providing current‐level modulators for identification of some types of DNA damage. In principle, any DNA base modification that can be converted chemically or enzymatically into an abasic site could be identified in this way.
Materialart:
Online-Ressource
ISSN:
0021-2148
,
1869-5868
DOI:
10.1002/ijch.v53.6/7
DOI:
10.1002/ijch.201300022
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2013
ZDB Id:
2066481-3
Permalink