In:
BMC Complementary and Alternative Medicine, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2014-12)
Abstract:
Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models. Methods The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D 2 (PGD 2 ) and leukotriene C 4 (LTC 4 ) on BMMC in vitro and PCA and OVA-induced AR models in vivo . Results EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC 50 value of 35.6 μg/ml. In addition, the generation of PGD 2 and LTC 4 was inhibited in BMMC in a concentration-dependent manner with IC 50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced. Conclusions Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.
Type of Medium:
Online Resource
ISSN:
1472-6882
DOI:
10.1186/1472-6882-14-54
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2014
detail.hit.zdb_id:
2050429-9
detail.hit.zdb_id:
3037610-5
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