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  • 1
    In: European Journal of Organic Chemistry, Wiley, Vol. 2015, No. 11 ( 2015-04), p. 2503-2516
    Abstract: The organocatalytic activities of highly substituted proline esters obtained through asymmetric [3+2] cycloadditions of azomethine ylides derived from glycine iminoesters have been analyzed by 19 F NMR and through kinetic isotope effects. Kinetic rate constants have been determined for unnatural proline esters incorporating different substituents. It has been found that exo ‐ L and endo ‐ L unnatural proline methyl esters yield opposite enantiomers in aldol reactions between cyclic ketones and aromatic aldehydes. The combined results reported in this study show subtle and remote effects that determine the organocatalytic behavior of these synthetic but readily available amino acid derivatives. These data can be used as design criteria for the development of new pyrrolidine‐based organocatalysts.
    Type of Medium: Online Resource
    ISSN: 1434-193X , 1099-0690
    URL: Issue
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    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1475010-7
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  • 2
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 85, No. 10 ( 2005-08-15), p. 1660-1671
    Abstract: Stage of lactation, use of bulk milk or milk from individual flocks, and cheese‐making in farmhouse or industrial factory are important factors affecting the production and quality of Idiazabal cheese. The volatile composition of cheese samples made from raw ewe's milk in farmhouses or industrial plants at two different times of the year and aged for 90 and 180 days was analysed by dynamic headspace coupled to GC‐MS. Short‐chain fatty acids, primary and secondary alcohols, methyl ketones and ethyl esters were the most abundant compounds in the aroma of Idiazabal cheese samples. Differences in the volatile composition were found between farmhouse and industrial cheeses made at different times of the year and ripened for 90 or 180 days. Likewise, the sensory profiles of the farmhouse and industrial cheeses were significantly different, regardless of the time of the year and ripening time. The results for the principal component analysis (PCA) performed on the sensory attributes of the cheese samples showed two PCs defined as ‘farmhouse flavour factor’ and ‘industrial flavour factor’. Farmhouse cheeses showed high scores for buttery, milky and toasty odours, and buttery and nutty flavours, whereas industrial cheeses showed high scores for sharp, rennet and brine odours, and rennet and rancid flavours. The percentages of methyl ketones such as 3‐hydroxy‐2‐butanone, 2‐butanone, 2‐pentanone and 2‐heptanone, and acids such as n ‐propanoic, 2‐methylpropanoic and 3‐methylbutanoic acids were higher in farmhouse cheeses than in industrial cheeses. On the other hand, the percentages of esters such as ethyl butanoate and ethyl hexanoate, and alcohols such as 3‐methyl‐1‐butanol, and acids like n ‐hexanoic acid were higher in industrial cheeses than in farmhouse cheeses. Relationships between sensory attributes and volatile compounds were studied on the basis of the differences found in sensory profile and volatile composition between farmhouse and industrial cheeses. Copyright © 2005 Society of Chemical Industry
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 2001807-1
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  • 3
    In: Journal of Dairy Research, Cambridge University Press (CUP), Vol. 76, No. 3 ( 2009-08), p. 301-307
    Abstract: Rennet coagulation parameters, curd texture and gross compositional variables were studied in ewes' raw milk samples from nine commercial flocks using different concentrate:forage ratios and grazing times. From early lactation to March flocks were fed concentrate pellets and hay whereas from April to the end of lactation flocks were allowed to graze from 6 to 19 h/day receiving concentrate supplementation in the morning and evening. Milk from late-lactation flocks, when allowed to graze, showed higher content of fat, dry matter, protein, casein, soluble protein, total calcium, curd firmness and curd resistance to compression than the milk from early lactation flocks. Higher total calcium content and lower fat content were found when the early lactation flocks were fed high concentrate:forage ratio than when the flocks were fed low ratio. Curd firmness were lower for milk from flocks fed high concentrate:forage ratio, and the curd resistance to compression was greater, than for milk from flocks fed low ratio. At late lactation, when flocks grazed for a long time per day the total calcium content was higher than when the flocks grazed for a short time per day. Principal component analysis showed that protein and fat content were highly correlated with coagulum and curd firmness, whereas total calcium content was highly correlated with curd resistance to compression, and milk pH with rennet coagulation time.
    Type of Medium: Online Resource
    ISSN: 0022-0299 , 1469-7629
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
    detail.hit.zdb_id: 2000010-8
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  • 4
    In: Meat Science, Elsevier BV, Vol. 72, No. 3 ( 2006-03), p. 486-495
    Type of Medium: Online Resource
    ISSN: 0309-1740
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2006
    detail.hit.zdb_id: 1491973-4
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  • 5
    In: Journal of Dairy Research, Cambridge University Press (CUP), Vol. 79, No. 4 ( 2012-11), p. 485-494
    Abstract: The objective of the present work was to study the differences in the fatty acid (FA) composition of raw sheep milk fat under commercial milk production conditions throughout lactation, in two consecutive years. Particular attention was placed on the C18:2 cis -9, trans -11 isomer, C18:1 trans -11 acid, and unsaturated FA as the feeding regimen of 10 commercial flocks of latxa dairy sheep changed from indoor feeding to part-time grazing conditions (from early spring) as traditionally practiced in the Basque Country (Northern Spain). Farms located at an altitude of between 600 and 700 m, in two different geographical areas with different rainfall were selected. Milk samples were collected monthly from late January (indoor feeding) until mid-, or end of, June (outdoor feeding), during two consecutive years. In spite of some interannual variability (most likely due to large differences in rainfall), the evolution of individual FA throughout lactation was comparable between years, indicating that it was reproducible under commercial milk production conditions. The average concentrations of C18:2 cis -9, trans -11 isomer and C18:1 trans -11 acid in milk from the commercial flocks increased about 200% during the transition period (end of March or early April until May), from indoor feeding (late January or early February until the end of March) to the outdoor period (early May to mid-June), remaining constant during the outdoor period (27·53 ± 9·32 μmol/g fat and 71·58 ± 20·53 μmol/g fat, respectively). Non-atherogenic FA comprised approximately 50% of all saturated FA at any time during lactation, whereas the milk atherogenicity index decreased significantly during the outdoor period. The Trolox-equivalent antioxidant capacity of the water-soluble milk fraction did not appear to be influenced by feeding management. The FA composition of cheeses made during the second year with milk from the indoor or outdoor periods reflected those of the corresponding milks. A principal components analysis clearly showed that differences in the milk FA composition were primarily due to outdoor grazing, with very little contribution from the geographical zone or the year.
    Type of Medium: Online Resource
    ISSN: 0022-0299 , 1469-7629
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2012
    detail.hit.zdb_id: 2000010-8
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  • 6
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 1997
    In:  Journal of Dairy Research Vol. 64, No. 3 ( 1997-08), p. 445-452
    In: Journal of Dairy Research, Cambridge University Press (CUP), Vol. 64, No. 3 ( 1997-08), p. 445-452
    Abstract: Two methods were compared for the determination of free fatty acids (FFA) from acetic to long-chain acids in samples with a large excess of triacylglycerols (TG) (1[ratio ]200, w/w), such as cheese and other dairy products. In method 1, after fat extraction, FFA were separated from TG by aminopropyl-bonded phase chromatography, injecting the fraction containing FFA directly into the gas chromatograph. In method 2, extracted fat was treated with tetramethylammonium hydroxide, the methyl ester derivatives being formed in the injector. Cheese samples and standard mixtures of FFA and TG in different proportions were analysed by both methods. The cheese sample contained 2·4 times more FFA when analysed by method 2 as compared with the result obtained with method 1. The composition of the standard mixtures analysed by method 1 closely reflected that of the original mixture and gave 90–100% recovery of FFA, regardless of their chain length and the ratio of FFA[ratio ] TG (1[ratio ]1 or 1[ratio ] 200, w/w). The composition of samples with a FFA[ratio ]TG ratio of 1[ratio ] 200 (w/v) was severely distorted (as compared with the original composition of the sample) when analysed by method 2. Varying recoveries of FFA were also obtained, the largest differences being found for the shorter-chain components. We conclude that the FFA fraction should be separated from the TG fraction before derivatization and chromatographic analysis, particularly for samples in which the FFA represent a minor fraction of the TG.
    Type of Medium: Online Resource
    ISSN: 0022-0299 , 1469-7629
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 1997
    detail.hit.zdb_id: 2000010-8
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  • 7
    In: Journal of Dairy Research, Cambridge University Press (CUP), Vol. 71, No. 3 ( 2004-08), p. 372-379
    Abstract: This work studied the addition of an adequate lipase to enhance lipolysis reactions and the development of piquant flavour and sharp odour in Idiazabal cheese, as an alternative to the use of lamb rennet paste. Cheeses were manufactured from bulk raw ewes' milk in 50 l vats with commercial bovine rennet and 80 lipase units of pregastric or 180 lipase units of fungal lipase and ripened for 180 days. A higher lipolytic activity was induced by lipase addition promoting strong changes in odour and flavour attributes. Both fungal and pregastric lipases increased the content of total free fatty acids (FFA), but the fungal lipase released mainly medium- and long-chain FFA. In contrast, the pregastric lipase preferably released short-chain FFA. Diglyceride (DG) content was considerably higher in cheeses made with added pregastric lipase compared with those made with fungal lipase or with no lipase. Monoglycerides (MG) were detected only in cheeses made with either lipase added, reaching comparable concentrations after ripening for 180 days. The cheeses made with pregastric lipase had the highest scores for odour and flavour intensity, and sharp and rennet odours, desirable attributes for the Idiazabal cheese made with lamb rennet paste. None of the texture attributes were significantly influenced by the concentrations of MG and DG in the cheeses made with either lipase. Thus, the pregastric lipase was more appropriate than the fungal lipase to develop a more traditionally-flavoured Idiazabal cheese.
    Type of Medium: Online Resource
    ISSN: 0022-0299 , 1469-7629
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2004
    detail.hit.zdb_id: 2000010-8
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  • 8
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 1998
    In:  Journal of Agricultural and Food Chemistry Vol. 46, No. 8 ( 1998-08-17), p. 3252-3256
    In: Journal of Agricultural and Food Chemistry, American Chemical Society (ACS), Vol. 46, No. 8 ( 1998-08-17), p. 3252-3256
    Type of Medium: Online Resource
    ISSN: 0021-8561 , 1520-5118
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 1998
    detail.hit.zdb_id: 1483109-0
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  • 9
    Online Resource
    Online Resource
    Wiley ; 2005
    In:  Journal of the Science of Food and Agriculture Vol. 85, No. 7 ( 2005-05), p. 1073-1083
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 85, No. 7 ( 2005-05), p. 1073-1083
    Abstract: Conjugated linoleic acid ( c 9, t 11 CLA) is a dietary fatty acid produced mainly by ruminant animals and exhibits promising health‐promoting biological effects. For lipid fatty acid composition analyses, including CLA, lipids must be pre‐treated so that the free and esterified fatty acids (triacylglycerols, phospholipids, etc) are available for determination. The most common treatments involve fatty acid methyl ester derivatives from relatively simple chemical reactions, but this becomes complicated when esterification of CLA is involved because of potential changes in its positional and geometrical isomers by reaction with certain reagents. In this review we explain concisely the advantages and disadvantages of the most popular methods (acid‐ and base‐catalysed methods) generally employed for total fatty acid derivatization and their determination on a gas chromatograph. Based on our experiences we put forward the (trimethylsilyl)diazomethane method as an alternative and successful approach for ruminant tissue lipid determinations. Copyright © 2005 Society of Chemical Industry
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 2001807-1
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  • 10
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 1994
    In:  Journal of Dairy Research Vol. 61, No. 2 ( 1994-05), p. 281-288
    In: Journal of Dairy Research, Cambridge University Press (CUP), Vol. 61, No. 2 ( 1994-05), p. 281-288
    Abstract: The effect of brining time and smoking on the free fatty acid content of Idiazabal cheese during ripening was examined. The main free fatty acids considered underwent at least some increase during the first stage of ripening before day 90 and tended to level off around a constant value towards the end of the ripening period. There were significant differences in free fatty acid levels during ripening among cheeses with different brining times and between smoked and unsmoked cheeses. Brining time and smoking exerted marked effects on lipolytic activity during cheese ripening, depending upon the free fatty acid involved and ripening time. In general, brining and smoking led to increases in free fatty acid levels at the end of the ripening period; the different behaviour of butyric acid may be due to a specific lipolytic activity.
    Type of Medium: Online Resource
    ISSN: 0022-0299 , 1469-7629
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 1994
    detail.hit.zdb_id: 2000010-8
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